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Invitrogen
CD68 Monoclonal Antibody (eBioY1/82A (Y1/82A)), PE, eBioscience™
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 20
CD68 Antibody (12-0689-42) in Flow
Knockout of CD68 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR904279_LV) and LentiArray Cas9 Lentivirus (Product # A32064). For Flow cytometry analysis, THP-1 CD68 Knock out cells were stained intracellularly using the intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with 0.5 µg Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience™ (Product # 12-4732-81, yellow histogram) or 0.5 µg CD68 Monoclonal Antibody (eBioY1/82A (Y1/82A)... View More
Product Details
12-0689-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse,
Rhesus monkey
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBioY1/82A (Y1/82A)
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10805746
Product Specific Information
Description: The eBioY1/82 antibody reacts with CD68 which belongs to the sialomucin family and is closely related to the family of lysosomal-associated membrane proteins (lamps) and scavenger receptor. CD68 is predominantly an intracellular protein, found mainly in the late endosomal compartment but can be detected in smaller amounts on the surface of cells of mainly myeloid derived cells; monocytes and macrophages, dendritic cells (and in some langerhan cells), neutrophils, basophils mast cells, myeloid progenitor cells, and a subset of CD34+ hemopoietic bone marrow progenitor cells. The function has not been fully elucidated but based on it's homolgy and structure suggests a role in antigen processing or presentation and protection of the lysosomal membrane from hydrolytic enzymes. Reports have also seen expression in activated T cells, and about 40% of peripheral blood B-lymphocytes and 50% of all B-ALL.
Staining with eBioY1/82A shows surface staining on B cells and a minor percentage of monocytes while intracellular staining results in about 50% of B cells and most monocytes.
Applications Reported: This eBioY1/82A (Y1/82A) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBioY1/82A (Y1/82A) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD68; CD68 antigen; Gp110; macrophage antigen CD68; Macrosialin; scavenger receptor class D, member 1
Gene Aliases: CD68; GP110; LAMP4; SCARD1
UniProt ID: (Human) P34810
Entrez Gene ID: (Human) 968
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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