Invitrogen

CD144 (VE-cadherin) Monoclonal Antibody (eBioBV13 (BV13)), PE, eBioscience™

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Antibody Testing Data (1)
Published Figures (21)

CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)

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CD144 (VE-cadherin) Antibody (12-1441-82) in Flow

Staining of bEND-3 cell line with 0.125 µg of Rat IgG1 K Isotype Contol PE (Product # 12-4301-82) (blue histogram) or 0.125 µg of Anti-Mouse CD144 (VE-Cadherin) PE (purple histogram). Total viable cells were used for analysis.

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Product Details

12-1441-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 14 publications 14 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioBV13 (BV13)

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1907346

Product Specific Information

Description: The eBioBV13 (BV13) monoclonal antibody reacts with mouse VE-Cadherin (CD144). VE-Cadherin is a 120 kDa member of the type II Cadherin family, characterized by the presence of 5 extracellular cadherin domains (ECD), and anchored to the actin cytoskeleton through their cytoplasmic tail. VE-Cadherin mediates homophilic adhesion between neighbouring endothelial cells and is localized within specialized structures at cell-cell contacts, called adherens junctions. VE-Cadherin is expressed constitutively throughout the entire vasculature, and is required for numerous endothelial cell functions including migration, survival, contact-dependent growth inhibition and endothelial cell assembly into tubular structures. Furthermore, it is thought that VE-Cadherin+CD45- cells from the yolk sac or aorta-gonad-mesonephros (AGM) have the potential to give rise to hematopoietic cells.

Applications Reported: This eBioBV13 (BV13) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioBV13 (BV13) antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer. VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Product References

Knockout RAGE alleviates cardiac fibrosis through repressing endothelial-to-mesenchymal transition (EndMT) mediated by autophagy.

Cell death & disease

Zhang L,He J,Wang J,Liu J,Chen Z,Deng B,Wei L,Wu H,Liang B,Li H,Huang Y,Lu L,Yang Z,Xian S,Wang L

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 12-1441-82) in Flow Cytometry

Tue May 11 00:00:00 EDT 2021

Interleukin 35 Delays Hindlimb Ischemia-Induced Angiogenesis Through Regulating ROS-Extracellular Matrix but Spares Later Regenerative Angiogenesis.

Frontiers in immunology

Fu H,Sun Y,Shao Y,Saredy J,Cueto R,Liu L,Drummer C,Johnson C,Xu K,Lu Y,Li X,Meng S,Xue ER,Tan J,Jhala NC,Yu D,Zhou Y,Bayless KJ,Yu J,Rogers TJ,Hu W,Snyder NW,Sun J,Qin X,Jiang X,Wang H,Yang X

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 12-1441-82) in Flow Cytometry

Fri Jul 09 00:00:00 EDT 2021

Role of Notch1 in the arterial specification and angiogenic potential of mouse embryonic stem cell-derived endothelial cells.

Stem cell research & therapy

Park JK,Lee TW,Do EK,Moon HJ,Kim JH

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 12-1441-82) in Flow Cytometry

Wed Jul 18 00:00:00 EDT 2018

Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis.

eLife

Bergiers I,Andrews T,Vargel Bölükbaşı Ö,Buness A,Janosz E,Lopez-Anguita N,Ganter K,Kosim K,Celen C,Itır Perçin G,Collier P,Baying B,Benes V,Hemberg M,Lancrin C

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 12-1441-82) in Flow Cytometry

Tue Mar 20 00:00:00 EDT 2018

Single-cell transcriptomics reveal the dynamic of haematopoietic stem cell production in the aorta.

Nature communications

Baron CS,Kester L,Klaus A,Boisset JC,Thambyrajah R,Yvernogeau L,Kouskoff V,Lacaud G,van Oudenaarden A,Robin C

Thu Jun 28 00:00:00 EDT 2018

CD157 Marks Tissue-Resident Endothelial Stem Cells with Homeostatic and Regenerative Properties.

Cell stem cell

Wakabayashi T,Naito H,Suehiro JI,Lin Y,Kawaji H,Iba T,Kouno T,Ishikawa-Kato S,Furuno M,Takara K,Muramatsu F,Weizhen J,Kidoya H,Ishihara K,Hayashizaki Y,Nishida K,Yoder MC,Takakura N

12-1441-82 was used in Flow cytometry/Cell sorting to show that tissue-resident VESCs display self-renewal capacity and that vascular regeneration potential exists in peripheral blood vessels.

Thu Mar 01 00:00:00 EST 2018

Medulloblastoma Genotype Dictates Blood Brain Barrier Phenotype.

Cancer cell

Phoenix TN,Patmore DM,Boop S,Boulos N,Jacus MO,Patel YT,Roussel MF,Finkelstein D,Goumnerova L,Perreault S,Wadhwa E,Cho YJ,Stewart CF,Gilbertson RJ

12-1441 was used in Flow cytometry/Cell sorting to demonstrate the biological basis of WNT-medulloblastoma treatment sensitivity: medulloblastoma genotype dictates tumour vessel phenotype, partially explaining disparate prognoses among subtypes.

Mon Apr 11 00:00:00 EDT 2016

Endothelial cation channel PIEZO1 controls blood pressure by mediating flow-induced ATP release.

The Journal of clinical investigation

Wang S,Chennupati R,Kaur H,Iring A,Wettschureck N,Offermanns S

12-1441 was used in Flow cytometry/Cell sorting to demonstrate that the cation channel, PIEZO1, is required for the regulation of nitric oxide formation, vascular tone, and blood pressure.

Thu Dec 01 00:00:00 EST 2016

Activation of the TGFβ pathway impairs endothelial to haematopoietic transition.

Scientific reports

Vargel Ö,Zhang Y,Kosim K,Ganter K,Foehr S,Mardenborough Y,Shvartsman M,Enright AJ,Krijgsveld J,Lancrin C

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 12-1441-82) in Flow Cytometry

Fri Feb 19 00:00:00 EST 2016

EphrinB2 regulates the emergence of a hemogenic endothelium from the aorta.

Scientific reports

Chen II,Caprioli A,Ohnuki H,Kwak H,Porcher C,Tosato G

12-1441 was used in Flow cytometry/Cell sorting to show that EphrinB2 regulates the emergence of a hemogenic endothelium from the aorta.

Thu Jun 02 00:00:00 EDT 2016

EphrinB2 regulates the emergence of a hemogenic endothelium from the aorta.

Scientific reports

Chen II,Caprioli A,Ohnuki H,Kwak H,Porcher C,Tosato G

12-1441 was used in Flow cytometry/Cell sorting to show that EphrinB2 regulates the emergence of a hemogenic endothelium from the aorta.

Thu Jun 02 00:00:00 EDT 2016

Suppression of ischemia in arterial occlusive disease by JNK-promoted native collateral artery development.

eLife

Ramo K,Sugamura K,Craige S,Keaney JF,Davis RJ

12-1441 was used in Flow cytometry/Cell sorting to investigate the role of the MLK-JNK signalling pathway in regulating protective mechanisms against ischemia in arterial occlusive disease.

Tue Aug 09 00:00:00 EDT 2016

Sox7 is regulated by ETV2 during cardiovascular development.

Stem cells and development

Behrens AN,Zierold C,Shi X,Ren Y,Koyano-Nakagawa N,Garry DJ,Martin CM

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 12-1441-82) in Flow Cytometry

Mon Sep 01 00:00:00 EDT 2014

The expression of Sox17 identifies and regulates haemogenic endothelium.

Nature cell biology

Clarke RL,Yzaguirre AD,Yashiro-Ohtani Y,Bondue A,Blanpain C,Pear WS,Speck NA,Keller G

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 12-1441-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2013

Developmental endothelial locus-1 (Del-1) mediates clearance of platelet microparticles by the endothelium.

Circulation

Dasgupta SK,Le A,Chavakis T,Rumbaut RE,Thiagarajan P

12-1441 was used in Flow cytometry/Cell sorting to show a physiological role for the developmental endothelial locus-1, in the clearance of phosphatidylserine-expressing microparticles by endothelium.

Tue Apr 03 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: 7B4/cadherin-5; Cadherin-5; CD144; Vascular endothelial cadherin; VE-cadherin

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Gene Aliases: 7B4; AA408225; Cd144; Cdh5; VE-Cad; Vec; VEcad; VECD

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UniProt ID: (Mouse) P55284

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Entrez Gene ID: (Mouse) 12562

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Function(s)

receptor binding calcium ion binding protein binding beta-catenin binding protein phosphatase binding ion channel binding metal ion binding cadherin

Process(es)

blood vessel maturation cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules negative regulation of cell proliferation single organismal cell-cell adhesion regulation of establishment of cell polarity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD144 (VE-cadherin) Monoclonal Antibody (eBioBV13 (BV13)), PE, eBioscience™

CD144 (VE-cadherin) Antibody (12-1441-82) in Flow

Product Details

12-1441-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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