Invitrogen

CD195 (CCR5) Monoclonal Antibody (HM-CCR5 (7A4)), PE, eBioscience™

2 Published Figures

11 References

View all (49) CCR5 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (2)

CD195 (CCR5) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 3

CD195 (CCR5) Antibody (12-1951-82) in Flow

Staining of FcR-blocked (Anti-Mouse CD16/CD32 Purified, Product # 14-0161-82) BALB/c thioglycolate-induced peritoneal exudate cells with Anti-Mouse CD11b FITC (Product # 11-0112-41) and 0.5 µg of Armenian Hamster IgG Isotype Control PE (Product # 12-4888-81) (left) or 0.5 µg of Anti-Mouse CD195 (CCR5) (right). Total viable cells were used for analysis.

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Product Details

12-1951-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 11 publications 11 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HM-CCR5 (7A4)

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_657684

Product Specific Information

Description: CCR5 is the major co-receptor for macrophage-tropic isolates of HIV-1. CCR5, also known as CD195, is a seven transmembrane chemokine receptor capable of binding RANTES, MIP-1 alpha and MIP-1 beta. Knockout mice show a decreased recruitment of macrophages and increased susceptibility to infection. Protein and mRNA has been found in macrophages from thioglycolate-treated and activated T cells.

The HM-CCR5(7A4) monoclonal antibody binds the N terminal extracellular domain of mouse CCR5 with no crossreactivity to human CCR5.

Applications Reported: This HM-CCR5 (7A4) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HM-CCR5 (7A4) antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal-exudate cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Human immunodeficiency virus (HIV) and related virus require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains fusion and entry of human white blood cells. CCR5 is required for the infection by HIV-1, HIV-2, and SIV. The beta-chemokines RANTES, MIP-alpha and MIP-beta are the ligands for CCR5 and prevent infection by M-tropic HIV-1. CCR5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells. The amino-terminal domain and the extracellular loops of CCR5 serve as HIV biding sites. CCR5 messenger RNA is expressed in lymphoid organs and monocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Visceral adipose tissue imparts peripheral macrophage influx into the hypothalamus.

Journal of neuroinflammation

Chen KE,Lainez NM,Nair MG,Coss D

12-1951-82 was used in Flow Cytometry to demonstrate a new connection between the adipose tissue and the hypothalamus in obesity that contributes to neuroinflammation and hypothalamic pathologies.

Mon Jun 21 00:00:00 EDT 2021

T helper 2 differentiation is necessary for development of lymphedema.

Translational research : the journal of laboratory and clinical medicine

Ly CL,Nores GDG,Kataru RP,Mehrara BJ

12-1951 was used in Flow cytometry/Cell sorting to analyse the effect of lymphatic injury in transgenic mice with mutations that cause defects in Th1 and Th2 cell generation (T-bet knockout or T-betKO and STAT6 knockout or STAT6KO mice, respectively).

Mon Apr 01 00:00:00 EDT 2019

Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells.

International journal of molecular sciences

Camps J,Grosemans H,Gijsbers R,Maes C,Sampaolesi M

12-1951 was used in Flow cytometry/Cell sorting to determine which chemokines are expressed in dystrophic muscles undergoing tissue remodelling.

Tue Mar 05 00:00:00 EST 2019

Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells.

International journal of molecular sciences

Camps J,Grosemans H,Gijsbers R,Maes C,Sampaolesi M

12-1951 was used in Flow cytometry/Cell sorting to determine which chemokines are expressed in dystrophic muscles undergoing tissue remodelling.

Tue Mar 05 00:00:00 EST 2019

The Transcription Factor NFATc1 Supports the Rejection of Heterotopic Heart Allografts.

Frontiers in immunology

Baur J,Otto C,Steger U,Klein-Hessling S,Muhammad K,Pusch T,Murti K,Wismer R,Germer CT,Klein I,Müller N,Serfling E,Avots A

12-1951 was used in Flow cytometry/Cell sorting to show that the activity of nuclear factors of activated T cells-c1 supports the acute rejection of heterotopic heart allografts.

Wed Nov 20 00:00:00 EST 2019

CD4⁺ T cells are activated in regional lymph nodes and migrate to skin to initiate lymphedema.

Nature communications

García Nores GD,Ly CL,Cuzzone DA,Kataru RP,Hespe GE,Torrisi JS,Huang JJ,Gardenier JC,Savetsky IL,Nitti MD,Yu JZ,Rehal S,Mehrara BJ

12-1951 was used in Flow cytometry/Cell sorting to demonstrate that blocking T cell release from lymph nodes using a sphingosine-1-phosphate receptor modulator prevents lymphedema.

Thu May 17 00:00:00 EDT 2018

Stability and function of regulatory T cells expressing the transcription factor T-bet.

Nature

Levine AG,Mendoza A,Hemmers S,Moltedo B,Niec RE,Schizas M,Hoyos BE,Putintseva EV,Chaudhry A,Dikiy S,Fujisawa S,Chudakov DM,Treuting PM,Rudensky AY

12-1951 was used in Flow cytometry/Cell sorting to demonstrate that T-bet+ Treg cells have an essential immunosuppressive function.

Thu Jun 15 00:00:00 EDT 2017

Tumor progression locus 2 (Tpl2) kinase promotes chemokine receptor expression and macrophage migration during acute inflammation.

The Journal of biological chemistry

Rowley SM,Kuriakose T,Dockery LM,Tran-Nguyen T,Gingerich AD,Wei L,Watford WT

12-1951 was used in Flow cytometry/Cell sorting to demonstrate an Tpl2-dependent mechanism for macrophage expression of certain chemokine receptors.

Fri May 30 00:00:00 EDT 2014

Mannose-capped lipoarabinomannan from Mycobacterium tuberculosis preferentially inhibits sphingosine-1-phosphate-induced migration of Th1 cells.

Journal of immunology (Baltimore, Md. : 1950)

Richmond JM,Lee J,Green DS,Kornfeld H,Cruikshank WW

12-1951 was used in Flow cytometry/Cell sorting to examine the ability of ManLAM to desensitize T cells to other chemoattractants during mycobacterial infection.

Sat Dec 15 00:00:00 EST 2012

The magnitude of the T cell response to a clinically significant dose of influenza virus is regulated by TRAIL.

Journal of immunology (Baltimore, Md. : 1950)

Brincks EL,Gurung P,Langlois RA,Hemann EA,Legge KL,Griffith TS

12-1951 was used in Flow cytometry/Cell sorting to study how TRAIL affects the T cell response and ultimately influences the outcome of viral infection.

Tue Nov 01 00:00:00 EDT 2011

Methyl gallate exhibits potent antitumor activities by inhibiting tumor infiltration of CD4+CD25+ regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Lee H,Lee H,Kwon Y,Lee JH,Kim J,Shin MK,Kim SH,Bae H

12-1951 was used in Flow cytometry/Cell sorting to study effect of Methyl gallate on Treg cells, leading to reduced tumour growth, due to reversing immune suppression.

Wed Dec 01 00:00:00 EST 2010

Infection with a helminth parasite attenuates autoimmunity through TGF-beta-mediated suppression of Th17 and Th1 responses.

Journal of immunology (Baltimore, Md. : 1950)

Walsh KP,Brady MT,Finlay CM,Boon L,Mills KH

12-1951 was used in Flow cytometry/Cell sorting to provide evidence that parasites can exert bystander immunosuppression of pathogenic T cells that mediate autoimmune diseases.

Sat Aug 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: C-C chemokine receptor type 5; C-C CKR-5; CC-CKR-5; CCR-5; CD195; CD195 antigen; chemokine (C-C) receptor 5; MIP-1 alpha receptor

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Gene Aliases: AM4-7; Ccr5; CD195; Cmkbr5

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UniProt ID: (Mouse) P51682

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Entrez Gene ID: (Mouse) 12774

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Function(s)

glycoprotein binding actin binding signal transducer activity G-protein coupled receptor activity chemokine receptor activity C-C chemokine receptor activity protein kinase binding C-C chemokine binding chemokine (C-C motif) ligand 5 binding

Process(es)

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CD195 (CCR5) Monoclonal Antibody (HM-CCR5 (7A4)), PE, eBioscience™

CD195 (CCR5) Antibody (12-1951-82) in Flow

Product Details

12-1951-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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