Invitrogen

EOMES Monoclonal Antibody (Dan11mag), PE, eBioscience™

FIGURE: 1 / 1

EOMES Antibody (12-4875-82) in Flow

Surface staining of C57BL/6 splenocytes with Anti-Mouse NK1-1 eFluor® 450 (Product # 48-5941-82) followed by intracellular staining with 0.25 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (left) or 0.25 µg of Anti-Mouse EOMES PE (right) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Total viable cells were used for analysis.

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Product Details

12-4875-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 18 publications 18 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Dan11mag

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1603275

Product Specific Information

Description: This Dan11mag antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R.

Applications Reported: This Dan11mag antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This Dan11mag antibody has been tested by intracellular flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

IL-18BP mediates the balance between protective and pathological immune responses to Toxoplasma gondii.

Cell reports

Clark JT,Weizman OE,Aldridge DL,Shallberg LA,Eberhard J,Lanzar Z,Wasche D,Huck JD,Zhou T,Ring AM,Hunter CA

12-4875-82 was used in Flow cytometry/Cell sorting to investigate the role of interleukin-18 (IL-18) and its binding protein (IL-18BP) in controlling Toxoplasma gondii infection.

Tue Mar 28 00:00:00 EDT 2023

Chronic CD27-CD70 costimulation promotes type 1-specific polarization of effector Tregs.

Frontiers in immunology

Bowakim-Anta N,Acolty V,Azouz A,Yagita H,Leo O,Goriely S,Oldenhove G,Moser M

12-4875-82 was used in Flow cytometry/Cell sorting to conclude that CD27 may regulate the development of Th1 immunity in peripheral tissues as well as the subsequent switch of the effector response into long-term memory.

Thu Apr 06 00:00:00 EDT 2023

Centrosome anchoring regulates progenitor properties and cortical formation.

Nature

Shao W,Yang J,He M,Yu XY,Lee CH,Yang Z,Joyner AL,Anderson KV,Zhang J,Tsou MB,Shi H,Shi SH

12-4875-82 was used in Immunohistochemistry to indicate a previously unknown role of the centrosome in regulating the mechanical features of neural progenitor cells and the size and configuration of the mammalian cerebral cortex.

Wed Apr 01 00:00:00 EDT 2020

Heterogenous Populations of Tissue-Resident CD8⁺ T Cells Are Generated in Response to Infection and Malignancy.

Immunity

Milner JJ,Toma C,He Z,Kurd NS,Nguyen QP,McDonald B,Quezada L,Widjaja CE,Witherden DA,Crowl JT,Shaw LA,Yeo GW,Chang JT,Omilusik KD,Goldrath AW

12-4875 was used in Flow cytometry/Cell sorting to identify discrete lineages of intestinal antigen-specific CD8+ T cells, including a Blimp1hiId3lo tissue-resident effector cell population most prominent in the early phase of acute viral and bacterial infections and a molecularly distinct Blimp1loId3hi tissue-resident memory population that subsequently accumulated at later infection time points.

Tue May 19 00:00:00 EDT 2020

Acetate coordinates neutrophil and ILC3 responses against C. difficile through FFAR2.

The Journal of experimental medicine

Fachi JL,Sécca C,Rodrigues PB,Mato FCP,Di Luccia B,Felipe JS,Pral LP,Rungue M,Rocha VM,Sato FT,Sampaio U,Clerici MTPS,Rodrigues HG,Câmara NOS,Consonni SR,Vieira AT,Oliveira SC,Mackay CR,Layden BT,Bortoluci KR,Colonna M,Vinolo MAR

12-4875 was used in Flow cytometry/Cell sorting to study the impact of a microbiota-derived metabolite, short-chain fatty acid acetate, on an acute mouse model of CDI.

Mon Mar 02 00:00:00 EST 2020

Cortical Neural Stem Cell Lineage Progression Is Regulated by Extrinsic Signaling Molecule Sonic Hedgehog.

Cell reports

Zhang Y,Liu G,Guo T,Liang XG,Du H,Yang L,Bhaduri A,Li X,Xu Z,Zhang Z,Li Z,He M,Tsyporin J,Kriegstein AR,Rubenstein JL,Yang Z,Chen B

12-4875 was used in Immunohistochemistry to demonstrate that GSX2+ cells are tri-potent intermediate progenitor cells (tri-IPCs), giving rise to OB-INs, cortical oligodendrocytes, and astrocytes.

Tue Mar 31 00:00:00 EDT 2020

Blockade of the AHR restricts a Treg-macrophage suppressive axis induced by L-Kynurenine.

Nature communications

Campesato LF,Budhu S,Tchaicha J,Weng CH,Gigoux M,Cohen IJ,Redmond D,Mangarin L,Pourpe S,Liu C,Zappasodi R,Zamarin D,Cavanaugh J,Castro AC,Manfredi MG,McGovern K,Merghoub T,Wolchok JD

12-4875 was used in Flow cytometry/Cell sorting to show that the AHR pathway is selectively active in IDO/TDO-overexpressing tumors and is associated with resistance to immune checkpoint inhibitors.

Tue Aug 11 00:00:00 EDT 2020

Blockade of CTLA-4 and PD-1 Enhances Adoptive T-cell Therapy Efficacy in an ICOS-Mediated Manner.

Cancer immunology research

Shi LZ,Goswami S,Fu T,Guan B,Chen J,Xiong L,Zhang J,Ng Tang D,Zhang X,Vence L,Blando J,Allison JP,Collazo R,Gao J,Sharma P

12-4875 was used in Flow cytometry/Cell sorting to provides a foundation of testing combinatorial therapy of ACT of CD8 T cells and dual blocking of CTLA-4 and PD-1 in patients with melanoma.

Fri Nov 01 00:00:00 EDT 2019

CPEB4-Dependent Neonate-Born Granule Cells Are Required for Olfactory Discrimination.

Frontiers in behavioral neuroscience

Tseng CS,Chou SJ,Huang YS

12-4875-82 was used in Immunohistochemistry to suggest that granule cells (GCs) born in the early postnatal olfactory bulb (OB) are required for olfactory discrimination.

Wed Sep 30 00:00:00 EDT 2020

A functional subset of CD8⁺ T cells during chronic exhaustion is defined by SIRPα expression.

Nature communications

Myers LM,Tal MC,Torrez Dulgeroff LB,Carmody AB,Messer RJ,Gulati G,Yiu YY,Staron MM,Angel CL,Sinha R,Markovic M,Pham EA,Fram B,Ahmed A,Newman AM,Glenn JS,Davis MM,Kaech SM,Weissman IL,Hasenkrug KJ

12-4875-82 was used in Flow Cytometry to demonstrate that even during exhaustion there is a subset of functional CD8+ T cells defined by surface expression of SIRPα.

Fri Feb 15 00:00:00 EST 2019

Haploinsufficiency of autism causative gene Tbr1 impairs olfactory discrimination and neuronal activation of the olfactory system in mice.

Molecular autism

Huang TN,Yen TL,Qiu LR,Chuang HC,Lerch JP,Hsueh YP

12-4875 was used in Immunohistochemistry-immunofluorescence to investigate the role of TBR1 in regulating olfaction and test whether D-cycloserine can also improve olfactory defects in Tbr1 mutant mice.

Thu Apr 11 00:00:00 EDT 2019

CD38-NAD⁺Axis Regulates Immunotherapeutic Anti-Tumor T Cell Response.

Cell metabolism

Chatterjee S,Daenthanasanmak A,Chakraborty P,Wyatt MW,Dhar P,Selvam SP,Fu J,Zhang J,Nguyen H,Kang I,Toth K,Al-Homrani M,Husain M,Beeson G,Ball L,Helke K,Husain S,Garrett-Mayer E,Hardiman G,Mehrotra M,Nishimura MI,Beeson CC,Bupp MG,Wu J,Ogretmen B,Paulos CM,Rathmell J,Yu XZ,Mehrotra S

12-4875 was used in Flow cytometry/Cell sorting to establish ex vivo culture conditions to generate hybrid Th1/17 cells, which persist long-term in vivo while maintaining effector function.

Tue Jan 09 00:00:00 EST 2018

Dependence of innate lymphoid cell 1 development on NKp46.

PLoS biology

Wang Y,Dong W,Zhang Y,Caligiuri MA,Yu J

12-4875 was used in Flow cytometry/Cell sorting to reveal that NKp46 selectively participates in the regulation of ILC1 development.

Sun Apr 01 00:00:00 EDT 2018

TCR-pMHC encounter differentially regulates transcriptomes of tissue-resident CD8 T cells.

European journal of immunology

Yoshizawa A,Bi K,Keskin DB,Zhang G,Reinhold B,Reinherz EL

12-4875 was used in Flow cytometry/Cell sorting to show TCR-pMHC interactions among TR and antigen presenting cells in a tissue milieu strongly impact CD8 T cell biology.

Mon Jan 01 00:00:00 EST 2018

Repeated Antigen Exposure Extends the Durability of Influenza-Specific Lung-Resident Memory CD8⁺ T Cells and Heterosubtypic Immunity.

Cell reports

Van Braeckel-Budimir N,Varga SM,Badovinac VP,Harty JT

12-4875 was used in Flow cytometry/Cell sorting to demonstrate that, relative to single virus exposure, repeated antigen exposure dramatically alters the dynamics of influenza-specific lung-resident primary memory CD8+ T cell populations.

Tue Sep 25 00:00:00 EDT 2018

Mitochondrial dysfunction underlies cognitive defects as a result of neural stem cell depletion and impaired neurogenesis.

Human molecular genetics

Khacho M,Clark A,Svoboda DS,MacLaurin JG,Lagace DC,Park DS,Slack RS

12-4875 was used in Immunocytochemistry-immunoflourescence to show that mitochondrial dysfunction impairs brain development, depletes the adult neural stem cell pool and impacts embryonic and adult neurogenesis.

Fri Sep 01 00:00:00 EDT 2017

Modulation of let-7 miRNAs controls the differentiation of effector CD8 T cells.

eLife

Wells AC,Daniels KA,Angelou CC,Fagerberg E,Burnside AS,Markstein M,Alfandari D,Welsh RM,Pobezinskaya EL,Pobezinsky LA

12-4875 was used in Flow cytometry/Cell sorting to identify a let-7-mediated mechanism that acts as a molecular brake controlling the magnitude of CD8 T-cell responses.

Mon Jul 24 00:00:00 EDT 2017

TGF-β receptor maintains CD4 T helper cell identity during chronic viral infections.

The Journal of clinical investigation

Lewis GM,Wehrens EJ,Labarta-Bajo L,Streeck H,Zuniga EI

12-4875 was used in Flow cytometry/Cell sorting to uncover an eomesodermin-driven CD4 T cell program that is continuously suppressed by TGF-β signalling.

Mon Oct 03 00:00:00 EDT 2016

IL-15 signaling promotes adoptive effector T-cell survival and memory formation in irradiation-induced lymphopenia.

Cell & bioscience

Xu A,Bhanumathy KK,Wu J,Ye Z,Freywald A,Leary SC,Li R,Xiang J

12-4875 was used in Flow cytometry/Cell sorting to investigate the mechanism for adoptively transferred effector T-cell survival and memory formation.

Mon May 09 00:00:00 EDT 2016

Phenotypic and functional characterization of the major lymphocyte populations in the fruit-eating bat Pteropus alecto.

Scientific reports

Martínez Gómez JM,Periasamy P,Dutertre CA,Irving AT,Ng JH,Crameri G,Baker ML,Ginhoux F,Wang LF,Alonso S

12487582 was used in flow cytometry to describe the phenotypic and functional characterization of T cell subsets, B and NK cells in the fruit-eating bat Pteropus alecto

Thu Nov 24 00:00:00 EST 2016

Interleukin-4 regulates eomesodermin in CD8+ T cell development and differentiation.

PloS one

Carty SA,Koretzky GA,Jordan MS

12-4875 was used in Flow cytometry/Cell sorting to investigate the signaling pathways required for IL-4 induction of Eomes and CD8(+) innate-like lymphocyte markers in murine CD8SP thymocytes and peripheral CD8(+) T cells.

Thu May 21 00:00:00 EDT 2015

The cytokines interleukin 27 and interferon-γ promote distinct Treg cell populations required to limit infection-induced pathology.

Immunity

Hall AO,Beiting DP,Tato C,John B,Oldenhove G,Lombana CG,Pritchard GH,Silver JS,Bouladoux N,Stumhofer JS,Harris TH,Grainger J,Wojno ED,Wagage S,Roos DS,Scott P,Turka LA,Cherry S,Reiner SL,Cua D,Belkaid Y,Elloso MM,Hunter CA

12-4875 was used in Flow cytometry/Cell sorting to study the promotion of distinct Treg cell populations by the cytokines IL-27 and IFN-y, and the role they play in limiting infection induced pathology.

Fri Sep 21 00:00:00 EDT 2012

Tbr2 deficiency in mitral and tufted cells disrupts excitatory-inhibitory balance of neural circuitry in the mouse olfactory bulb.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Mizuguchi R,Naritsuka H,Mori K,Mao CA,Klein WH,Yoshihara Y

12-4875-82 was used in Immunohistochemistry to study the molecular mechanisms underlying the formation of functional neural circuitry among various types of neurons inside the olfactory bulb.

Wed Jun 27 00:00:00 EDT 2012

IFN-alpha beta and self-MHC divert CD8 T cells into a distinct differentiation pathway characterized by rapid acquisition of effector functions.

Journal of immunology (Baltimore, Md. : 1950)

Marshall HD,Prince AL,Berg LJ,Welsh RM

Sun Aug 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: Eomesodermin homolog; T-box brain protein 2; T-brain-2

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Gene Aliases: C77258; Eomes; TBR-2; Tbr2

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UniProt ID: (Mouse) O54839

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Entrez Gene ID: (Mouse) 13813

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II activating transcription factor binding transcriptional repressor activity, RNA polymerase II transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding Rel homology transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter endoderm formation mesoderm formation endodermal cell fate specification blastocyst development trophectodermal cell differentiation CD8-positive, alpha-beta T cell differentiation involved in immune response immune system process transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter multicellular organism development gastrulation brain development cardioblast differentiation regulation of gene expression stem cell population maintenance olfactory bulb development cerebral cortex regionalization cerebral cortex neuron differentiation cell differentiation interferon-gamma production skeletal muscle cell differentiation positive regulation of cell differentiation regulation of neuron differentiation positive regulation of transcription from RNA polymerase II promoter regulation of neurogenesis cell differentiation involved in embryonic placenta development mesodermal to mesenchymal transition involved in gastrulation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EOMES Monoclonal Antibody (Dan11mag), PE, eBioscience™

EOMES Antibody (12-4875-82) in Flow

Product Details

12-4875-82

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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