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Invitrogen
CD137 Ligand (4-1BB Ligand) Monoclonal Antibody (TKS-1), PE, eBioscience™
FIGURE: 1 / 1
CD137 Ligand (4-1BB Ligand) Antibody (12-5901-82) in Flow
Staining of 3-day unstimulated (left) and 3-day Anti-Mouse IgM and Anti-Mouse CD40-stimulated (right) mouse splenocytes with 0.5 µg of Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (blue histogram) or 0.5 µg of Anti-Mouse CD137 Ligand (4-1BB Ligand) PE (Product # 12-5901-82) (purple histogram). Total viable CD45R (B220)+ cells were used for analysis.
Product Details
12-5901-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
TKS-1
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_466033
Product Specific Information
Description: The TKS-1 monoclonal antibody reacts with mouse 4-1BB Ligand (4-1BBL), a type II transmembrane protein and a member of the tumor necrosis factor superfamily. 4-1BBL is expressed by activated antigen presenting cells including B cells and macrophages. Interaction of 4-1BBL with 4-1BB (CDw137) is reported to deliver a costimulatory signal important in the T-APC interaction.
Applications Reported: The TKS-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The TKS-1 antibody has been tested by flow cytometric analysis of resting and 2-3 day (IgM+CD40)-activated mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD137 ligand (CD137L) is a type II membrane protein and part of the TNF superfamily. CD137L is a co-stimulatory molecule that is expressed on antigen presenting cells (DCs, monocytes/macrophages, B cells) and is upregulated upon activation. Its receptor, CD137/4-1BB, is found on a variety of cells, including inflamed endothelial cells, where its expression enhances extravasation of CD137L expressing monocytes. Activated T cells also express CD137, and engagement with CD137L enhances T cell proliferation, IL-2 secretion, survival, and cytotoxic activity. CD137L is also expressed on a majority of B-cell lymphomas, including mantle cell lymphoma, follicular lymphoma, and diffuse large B-cell lymphoma (but it is lacking on Hodgkin lymphoma and T-cell lymphoma). CD137L is also expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: 4 1 BB Ligand; 4 1 BBL; 4-1BB ligand; 4-1BBL; 41BB Ligand; 41BBL; CD 137 Ligand; CD 137L; CD137 Ligand; CDw137L; TNFRSF9; Tumor necrosis factor ligand superfamily member 9
Gene Aliases: 4-1BB-L; 4-1BBL; AI848817; Cd137l; Cd157l; Ly63l; Tnfsf9
UniProt ID: (Mouse) P41274
Entrez Gene ID: (Mouse) 21950
immune response
cell-cell signaling
positive regulation of interferon-gamma production
positive regulation of interleukin-12 production
positive regulation of interleukin-6 production
positive regulation of activated T cell proliferation
regulation of cell proliferation
myeloid dendritic cell differentiation
positive regulation of cytotoxic T cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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