Invitrogen

CD276 (B7-H3) Monoclonal Antibody (M3.2D7), PE, eBioscience™

1 Published Figure

12 References

View all (94) B7-H3 (CD276) antibodies

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Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

CD276 (B7-H3) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 2

CD276 (B7-H3) Antibody (12-5973-82) in Flow

Staining of non-transfected (left) and mouse CD276-transfected (right) 293T cells with 0.25 µg of Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (open histogram) or 0.25 µg of Anti-Mouse CD276 (B7-H3) PE (filled histogram). Total cells were used for analysis.

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Product Details

12-5973-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 11 publications 11 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

M3.2D7

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466080

Product Specific Information

Description: The M3.2D7 monoclonal antibody was generated against and reacts with mouse B7-H3, a member of the B7 family of the Ig superfamily proteins. B7-H3 is reported to be expressed by antigen presenting cells and developing bone during embryogenesis. The ligand for B7-H3 has not yet been identified and it is thought that B7-H3 down-regulates T cell functions by engaging an unknown counter receptor on T cells. M3.2D7 stains mouse B7-H3 transfected cells and not mouse spleen cells. M3.2D7 also stains human B7-H3 transfected cells.

Applications Reported:The M3.2D7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: Has been tested by flow cytometric analysis of mouse B7-H3 transfected cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD276 is a member of the B7 family of co-stimulatory molecules also known as B7-H3. CD276 is a type I transmembrane protein that induces the proliferation of CD4+ and CD8+ T cells, enhances the generation of cytotoxic T cells and selectively stimulates the production of interferon gamma. Expression of CD276 can be induced on dendritic cells and monocytes by inflammatory cytokines, and is also widely expressed in peripheral tissues including the heart, kidney, testis and colon. In humans, CD276 exists as two isoforms which result from gene duplication and differential splicing. CD276 is reported to have therapeutic potential for the regulation of cell-mediated immune responses to cancer, particularly in conjunction with anti-angiogenic therapy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Dysfunction of Natural Killer Cells by FBP1-Induced Inhibition of Glycolysis during Lung Cancer Progression.

Cell metabolism

Cong J,Wang X,Zheng X,Wang D,Fu B,Sun R,Tian Z,Wei H

12-5973 was used in Flow cytometry/Cell sorting to identify novel mechanisms in natural killer cell dysfunction.

Tue Aug 07 00:00:00 EDT 2018

A novel subset of B7-H3⁺CD14⁺HLA-DR^-/low myeloid-derived suppressor cells are associated with progression of human NSCLC.

Oncoimmunology

Zhang G,Huang H,Zhu Y,Yu G,Gao X,Xu Y,Liu C,Hou J,Zhang X

12-5973 was used in Flow cytometry/Cell sorting to assess the contribution of myeloid-derived suppressor cells to tumour progression and metastasis.

Sun Feb 01 00:00:00 EST 2015

A novel subset of B7-H3⁺CD14⁺HLA-DR^-/low myeloid-derived suppressor cells are associated with progression of human NSCLC.

Oncoimmunology

Zhang G,Huang H,Zhu Y,Yu G,Gao X,Xu Y,Liu C,Hou J,Zhang X

12-5973 was used in Flow cytometry/Cell sorting to assess the contribution of myeloid-derived suppressor cells to tumour progression and metastasis.

Sun Feb 01 00:00:00 EST 2015

Regulatory T cells stimulate B7-H1 expression in myeloid-derived suppressor cells in ret melanomas.

The Journal of investigative dermatology

Fujimura T,Ring S,Umansky V,Mahnke K,Enk AH

12-5973 was used in Flow cytometry/Cell sorting to suggest the existence of a cross-talk between regulatory T-cells and myeloid-derived suppressor cells.

Sun Apr 01 00:00:00 EDT 2012

A novel monoclonal antibody against mouse B7-H3 developed in rats.

Hybridoma (2005)

Yan R,Yang S,Sun J,Chen X,Zhang G,Feng P,Zhang X

13-5973 was used in Western Blotting to characterise the biological functions of a novel anti-mouse B7-H3 monoclonal antibody.

Wed Aug 01 00:00:00 EDT 2012

A novel monoclonal antibody against mouse B7-H3 developed in rats.

Hybridoma (2005)

Yan R,Yang S,Sun J,Chen X,Zhang G,Feng P,Zhang X

13-5973 was used in Western Blotting to characterise the biological functions of a novel anti-mouse B7-H3 monoclonal antibody.

Wed Aug 01 00:00:00 EDT 2012

Antigen-specific immunity and cross-priming by epithelial ovarian carcinoma-induced CD11b(+)Gr-1(+) cells.

Journal of immunology (Baltimore, Md. : 1950)

Tomihara K,Guo M,Shin T,Sun X,Ludwig SM,Brumlik MJ,Zhang B,Curiel TJ,Shin T

12-5973 was used in Flow cytometry/Cell sorting to investigate CD11b(+)Gr-1(+) cells found in epithelial ovarian cancer-bearing mice, which are induced by ovarian cancer cells and undergo antigen-specific immunity and cross-priming.

Tue Jun 01 00:00:00 EDT 2010

A critical role of IFNgamma in priming MSC-mediated suppression of T cell proliferation through up-regulation of B7-H1.

Cell research

Sheng H,Wang Y,Jin Y,Zhang Q,Zhang Y,Wang L,Shen B,Yin S,Liu W,Cui L,Li N

12-5973 was used in Flow cytometry/Cell sorting to investigate the role that IFNγ plays in mesenchymal stem cell mediated suppression of T cell proliferation.

Fri Aug 01 00:00:00 EDT 2008

B7-H1 on hepatocytes facilitates priming of specific CD8 T cells but limits the specific recall of primed responses.

Gastroenterology

Wahl C,Bochtler P,Chen L,Schirmbeck R,Reimann J

12-5973 was used in Flow cytometry/Cell sorting to study the relationship between B7-H1 on hepatocytes and specific CD8 T cells.

Mon Sep 01 00:00:00 EDT 2008

Terminal complement complex C5b-9-treated human monocyte-derived dendritic cells undergo maturation and induce Th1 polarization.

European journal of immunology

Chen Y,Yang C,Jin N,Xie Z,Tang Y,Fei L,Jia Z,Wu Y

Mon Jan 01 00:00:00 EST 2007

Phenotypic differences between Th1 and Th17 cells and negative regulation of Th1 cell differentiation by IL-17.

Journal of leukocyte biology

Nakae S,Iwakura Y,Suto H,Galli SJ

Tue May 01 00:00:00 EDT 2007

Sinomenine promotes differentiation but impedes maturation and co-stimulatory molecule expression of human monocyte-derived dendritic cells.

International immunopharmacology

Chen Y,Yang C,Jin N,Xie Z,Fei L,Jia Z,Wu Y

Wed Aug 01 00:00:00 EDT 2007

Type I IFN-producing CD4 Valpha14i NKT cells facilitate priming of IL-10-producing CD8 T cells by hepatocytes.

Journal of immunology (Baltimore, Md. : 1950)

Wahl C,Bochtler P,Schirmbeck R,Reimann J

12-5973-82 was used in Flow Cytometry to determine whether hepatic NKT cells can modulate the phenotype of CD8 T cells during their priming.

Thu Feb 15 00:00:00 EST 2007

Bioinformatics

Protein Aliases: B7 homolog 3; B7-H3; CD276; CD276 antigen; Costimulatory molecule; sB7 H3; sB7H3; sCD276; soluble B7 H3; soluble B7H3; soluble CD276

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Gene Aliases: 6030411F23Rik; AU016588; B7h3; B7RP-2; Cd276

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UniProt ID: (Mouse) Q8VE98

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Entrez Gene ID: (Mouse) 102657

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Function(s)

receptor binding immunoglobulin receptor superfamily

Process(es)

positive regulation of bone mineralization positive regulation of T cell proliferation T cell activation negative regulation of T cell proliferation negative regulation of interferon-gamma biosynthetic process positive regulation of interferon-gamma biosynthetic process negative regulation of interleukin-2 biosynthetic process positive regulation of osteoblast differentiation negative regulation of inflammatory response regulation of immune response positive regulation of interleukin-2 secretion

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CD276 (B7-H3) Monoclonal Antibody (M3.2D7), PE, eBioscience™

CD276 (B7-H3) Antibody (12-5973-82) in Flow

Product Details

12-5973-82

Western Blot (WB)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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