Invitrogen

CD275 (B7-H2) Monoclonal Antibody (HK5.3), PE, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 Published Figures

24 References

View all (62) CD275 (B7-H2) antibodies

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Datasheet

Protocols

Questions & Answers

CD275 (B7-H2) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 4

CD275 (B7-H2) Antibody (12-5985-82) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.06 µg of Rat IgG2a K Isotype Control PE (left) or 0.06 µg of Anti-Mouse CD275 (B7-H2) PE (right). Total viable cells were used for analysis.

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Product Details

12-5985-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 22 publications 22 publications

Neutralization (Neu)

View 1 publication 1 publication

Inhibition Assays (IA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HK5.3

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466094

Product Specific Information

Description: The HK5.3 monoclonal antibody reacts with mouse B7RP-1, also known as B7h, B7-H2, GL50 and ICOS Ligand. B7RP-1, a member of the B7 family, has a predicted molecular weight of ~40 kDa and belongs to the Ig superfamily. Mouse B7RP-1 is expressed by antigen presenting cells including B cells, monocytes/macrophages and dendritic cells. B7RP-1 binds to the ICOS molecule (AILIM, CRP-1) expressed by activated T cells. The interaction of ICOS/B7RP-1 plays an important role in the T cell costimulation pathway.

Applications Reported: The HK5.3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The HK5.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Inducible co-stimulator ligand (ICOSL) is a specific ligand on antigen-presenting cells and cells of the peripheral tissue that binds to the inducible co-stimulator receptor (ICOS). ICOS belongs to the CD28/CD152 receptor family that regulates T-cell activation and function. ICOSL is expressed on monocytes, dendritic cells and B cells and can be induced by inflammatory stimuli in peripheral tissue. Binding to ICOSL delivers a co-stimulatory signal for T cell proliferation and cytokine secretion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Glioblastomas acquire myeloid-affiliated transcriptional programs via epigenetic immunoediting to elicit immune evasion.

Cell

Gangoso E,Southgate B,Bradley L,Rus S,Galvez-Cancino F,McGivern N,Güç E,Kapourani CA,Byron A,Ferguson KM,Alfazema N,Morrison G,Grant V,Blin C,Sou I,Marques-Torrejon MA,Conde L,Parrinello S,Herrero J,Beck S,Brandner S,Brennan PM,Bertone P,Pollard JW,Quezada SA,Sproul D,Frame MC,Serrels A,Pollard SM

12-5985-82 was used in Flow Cytometry to conclude that epigenetic immunoediting may drive an acquired immune evasion program in the most aggressive mesenchymal GBM subtype by reshaping the tumor immune microenvironment.

Thu Apr 29 00:00:00 EDT 2021

PD-L1 in tumor microenvironment mediates resistance to oncolytic immunotherapy.

The Journal of clinical investigation

Zamarin D,Ricca JM,Sadekova S,Oseledchyk A,Yu Y,Blumenschein WM,Wong J,Gigoux M,Merghoub T,Wolchok JD

Mon Apr 02 00:00:00 EDT 2018

Lysis-independent potentiation of immune checkpoint blockade by oncolytic virus.

Oncotarget

Oseledchyk A,Ricca JM,Gigoux M,Ko B,Redelman-Sidi G,Walther T,Liu C,Iyer G,Merghoub T,Wolchok JD,Zamarin D

Published figure using CD275 (B7-H2) monoclonal antibody (Product # 12-5985-82) in Flow Cytometry

Tue Jun 19 00:00:00 EDT 2018

T-Bet⁺ IgM Memory Cells Generate Multi-lineage Effector B Cells.

Cell reports

Kenderes KJ,Levack RC,Papillion AM,Cabrera-Martinez B,Dishaw LM,Winslow GM

12-5985 was used in Flow cytometry/Cell sorting to propose that T-bet+ IgM memory cells can differentiate into any effector B cell lineage to provide long-term multifunctional immunity.

Tue Jul 24 00:00:00 EDT 2018

ILC2s regulate adaptive Th2 cell functions via PD-L1 checkpoint control.

The Journal of experimental medicine

Schwartz C,Khan AR,Floudas A,Saunders SP,Hams E,Rodewald HR,McKenzie ANJ,Fallon PG

12-5985 was used in Flow cytometry/Cell sorting to study how group 2 innate lymphoid cells regulate adaptive Th2 cell functions.

Mon Sep 04 00:00:00 EDT 2017

Batf3-dependent CD8α⁺ Dendritic Cells Aggravates Atherosclerosis via Th1 Cell Induction and Enhanced CCL5 Expression in Plaque Macrophages.

EBioMedicine

Li Y,Liu X,Duan W,Tian H,Zhu G,He H,Yao S,Yi S,Song W,Tang H

Sat Apr 01 00:00:00 EDT 2017

Intratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity.

Nature communications

Zamarin D,Holmgaard RB,Ricca J,Plitt T,Palese P,Sharma P,Merghoub T,Wolchok JD,Allison JP

12-5985 was used in Flow cytometry/Cell sorting to highlight that intratumoral immunomodulation with an oncolytic virus expressing a rationally selected ligand can be an effective strategy to drive systemic efficacy of immune checkpoint blockade.

Mon Feb 13 00:00:00 EST 2017

High Fat Diet Inhibits Dendritic Cell and T Cell Response to Allergens but Does Not Impair Inhalational Respiratory Tolerance.

PloS one

Pizzolla A,Oh DY,Luong S,Prickett SR,Henstridge DC,Febbraio MA,O'Hehir RE,Rolland JM,Hardy CL

12-5985 was used in Flow cytometry/Cell sorting to study the relationship between a high fat diet and allergic asthma using an induced-respiratory tolerance model in mice.

Wed Jul 26 00:00:00 EDT 2017

Lignin-rich enzyme lignin (LREL), a cellulase-treated lignin-carbohydrate derived from plants, activates myeloid dendritic cells via Toll-like receptor 4 (TLR4).

The Journal of biological chemistry

Tsuji R,Koizumi H,Aoki D,Watanabe Y,Sugihara Y,Matsushita Y,Fukushima K,Fujiwara D

12-5985 was used in Flow cytometry/Cell sorting to investigate the role of lignin-rich enzyme lignin in dendritic cells.

Fri Feb 13 00:00:00 EST 2015

CagA-dependent downregulation of B7-H2 expression on gastric mucosa and inhibition of Th17 responses during Helicobacter pylori infection.

Journal of immunology (Baltimore, Md. : 1950)

Lina TT,Pinchuk IV,House J,Yamaoka Y,Graham DY,Beswick EJ,Reyes VE

12-5985 was used in Flow cytometry/Cell sorting to investigate the impact of H.pylori CagA on the modulation of the expression of the T cell costimulator B7-H2 by gastric epithelial cells.

Tue Oct 01 00:00:00 EDT 2013

CD8α⁺β⁻ and CD8α⁺β⁺ plasmacytoid dendritic cells induce Foxp3⁺ regulatory T cells and prevent the induction of airway hyper-reactivity.

Mucosal immunology

Lombardi V,Speak AO,Kerzerho J,Szely N,Akbari O

12-5985 was used in Flow cytometry/Cell sorting to reveal three functional and phenotypically distinct populations of mice plasmacytoid dendritic cells.

Sun Jul 01 00:00:00 EDT 2012

Cytip regulates dendritic-cell function in contact hypersensitivity.

European journal of immunology

Heib V,Sparber F,Tripp CH,Ortner D,Stoitzner P,Heufler C

12-5985 was used in Flow cytometry/Cell sorting to evaluate the functional consequences of cytohesin-interacting protein (Cytip) deficiency in dendritic cells using Cytip knockout mice.

Thu Mar 01 00:00:00 EST 2012

OX40 ligand and programmed cell death 1 ligand 2 expression on inflammatory dendritic cells regulates CD4 T cell cytokine production in the lung during viral disease.

Journal of immunology (Baltimore, Md. : 1950)

Wythe SE,Dodd JS,Openshaw PJ,Schwarze J

12-5985 was used in Flow cytometry/Cell sorting to study the regulation of CD4 T cell cytokine release during viral lung disease, by OX40 and PD-L2 expression on inflammatory dendritic cells.

Wed Feb 15 00:00:00 EST 2012

Resident CD11b(+)Ly6C(-) lung dendritic cells are responsible for allergic airway sensitization to house dust mite in mice.

PloS one

Mesnil C,Sabatel CM,Marichal T,Toussaint M,Cataldo D,Drion PV,Lekeux P,Bureau F,Desmet CJ

12-5985 was used in Flow cytometry/Cell sorting to determine whether specific conventional dendritic cell subsets are involved in allergic airway sensitisation.

Mon Jun 24 00:00:00 EDT 2013

Retinal astrocytes pretreated with NOD2 and TLR2 ligands activate uveitogenic T cells.

PloS one

Jiang G,Sun D,Kaplan HJ,Shao H

12-5985 was used in Flow cytometry/Cell sorting to investigate the activation of uveitogenic T cells via the pretreatment of retinal astrocytes with NOD2 and TLR2 ligands.

Tue Mar 19 00:00:00 EDT 2013

Retinal astrocytes pretreated with NOD2 and TLR2 ligands activate uveitogenic T cells.

PloS one

Jiang G,Sun D,Kaplan HJ,Shao H

12-5985 was used in Flow cytometry/Cell sorting to investigate the activation of uveitogenic T cells via the pretreatment of retinal astrocytes with NOD2 and TLR2 ligands.

Tue Mar 19 00:00:00 EDT 2013

Retinal astrocytes pretreated with NOD2 and TLR2 ligands activate uveitogenic T cells.

PloS one

Jiang G,Sun D,Kaplan HJ,Shao H

12-5985 was used in Flow cytometry/Cell sorting to investigate the activation of uveitogenic T cells via the pretreatment of retinal astrocytes with NOD2 and TLR2 ligands.

Tue Mar 19 00:00:00 EDT 2013

Antigen-specific immunity and cross-priming by epithelial ovarian carcinoma-induced CD11b(+)Gr-1(+) cells.

Journal of immunology (Baltimore, Md. : 1950)

Tomihara K,Guo M,Shin T,Sun X,Ludwig SM,Brumlik MJ,Zhang B,Curiel TJ,Shin T

12-5985 was used in Flow cytometry/Cell sorting to investigate CD11b(+)Gr-1(+) cells found in epithelial ovarian cancer-bearing mice, which are induced by ovarian cancer cells and undergo antigen-specific immunity and cross-priming.

Tue Jun 01 00:00:00 EDT 2010

The herbal medicine compound falcarindiol from Notopterygii Rhizoma suppresses dendritic cell maturation.

The Journal of pharmacology and experimental therapeutics

Mitsui S,Torii K,Fukui H,Tsujimura K,Maeda A,Nose M,Nagatsu A,Mizukami H,Morita A

12-5985 was used in Flow cytometry/Cell sorting to study the effects of falcarindiol on dendritic cell function.

Tue Jun 01 00:00:00 EDT 2010

CD4 T-cell suppression by cells from Toxoplasma gondii-infected retinas is mediated by surface protein PD-L1.

Infection and immunity

Charles E,Joshi S,Ash JD,Fox BA,Farris AD,Bzik DJ,Lang ML,Blader IJ

12-5985 was used in Flow cytometry/Cell sorting to investigate the mechanisms by which the retina protects itself from CD4 T-cell-mediated immune damage in retinal immune responses.

Sun Aug 01 00:00:00 EDT 2010

Lung vascular endothelial growth factor expression induces local myeloid dendritic cell activation.

Clinical immunology (Orlando, Fla.)

Chapoval SP,Lee CG,Tang C,Keegan AD,Cohn L,Bottomly K,Elias JA

12-5985 was used in Flow cytometry/Cell sorting to demonstrate how lung vascular endothelial growth factor (VEGF) expression affects myeloid dendritic cells.

Tue Sep 01 00:00:00 EDT 2009

Absence of IFN-beta impairs antigen presentation capacity of splenic dendritic cells via down-regulation of heat shock protein 70.

Journal of immunology (Baltimore, Md. : 1950)

Zietara N,Łyszkiewicz M,Gekara N,Puchałka J,Dos Santos VA,Hunt CR,Pandita TK,Lienenklaus S,Weiss S

12-5985 was used in Flow cytometry/Cell sorting to investigate the role of low level production of IFN, showing that its absence impairs antigen presentation capacity of splenic dendritic cells.

Wed Jul 15 00:00:00 EDT 2009

Allostimulatory activity of bone marrow-derived plasmacytoid dendritic cells is independent of indoleamine dioxygenase but regulated by inducible costimulator ligand expression.

Human immunology

Colvin BL,Sumpter TL,Tokita D,Salati J,Mellor AL,Thomson AW

12-5985 was used in Flow cytometry/Cell sorting to implicate inducible costimulator ligand-induced IL-10 in the regulation of bone marrow-derived plasmacytoid dendritic cell function.

Fri May 01 00:00:00 EDT 2009

Migratory Langerhans cells in mouse lymph nodes in steady state and inflammation.

The Journal of investigative dermatology

Stoitzner P,Tripp CH,Douillard P,Saeland S,Romani N

12-5985 was used in Flow cytometry/Cell sorting to determine that the overall phenotype of Langerhans cells is not an indicator for their immunogenic or tolerogenic potential.

Fri Jul 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: B7 homolog 2; B7-H2; B7-like protein Gl50; B7-related protein 1; B7RP-1; CD275; ICOS ligand; LICOS

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Gene Aliases: AU044799; B7-H2; B7h; B7h2; B7RP-1; B7rp1; BG071784; GI50; GL50; GL50-B; ICOS-L; Icosl; Icoslg; LICOS; Ly115l; mKIAA0653

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UniProt ID: (Mouse) Q9JHJ8

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Entrez Gene ID: (Mouse) 50723

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Function(s)

receptor binding protein binding immunoglobulin receptor superfamily

Process(es)

immune system process signal transduction positive regulation of activated T cell proliferation T cell activation B cell activation isotype switching positive regulation of interleukin-4 biosynthetic process

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CD275 (B7-H2) Monoclonal Antibody (HK5.3), PE, eBioscience™

CD275 (B7-H2) Antibody (12-5985-82) in Flow

Product Details

12-5985-82

Flow Cytometry (Flow)

Neutralization (Neu)

Inhibition Assays (IA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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