Invitrogen

CD273 (B7-DC) Monoclonal Antibody (TY25), PE, eBioscience™

CD273 (B7-DC) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 3

CD273 (B7-DC) Antibody (12-5986-82) in Flow

Staining of non-transfected (left) and mouse B7-DC-transfected (right) cells with 0.25 µg of Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (open histogram) or 0.06 µg of Anti-Mouse CD273 (B7-DC) PE (filled histogram). Total viable cells were used for analysis.

Product Image Gallery

Product Details

12-5986-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 31 publications 31 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TY25

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466097

Product Specific Information

Description: The TY25 monoclonal antibody reacts with mouse B7-DC/PD-L2. B7-DC, a member of the B7 family, has a predicted molecular weight of ~25 kDa and belongs to the Ig superfamily. The mouse B7-DC has a short cytoplasmic tail (4aa). B7-DC is primarily expressed by sub-populations of dendritic cells, monocytes and macrophages. Although B7-DC has structural and sequence similarities to the B7 family, it does not bind CD28/CTLA-4, but binds PD-1. The interactions between PD-1 and B7-DC/PD-L2 have been reported to be involved in costimulation or suppression of T cell proliferation depending on state of cellular activation. TY25 is a useful tool to study the exact function of B7-DC/PD-L2 in APC/T cell interaction and to characterize the expression pattern of this molecule in mouse.

Applications Reported: The TY25 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The TY25 antibody has been tested by flow cytometric analysis of mouse splenocytes and B7-DC transfected cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Programmed death-ligand 2 (PD-L2), or B7-DC, is a member of the B7 ligand family within the immunoglobulin superfamily that, along with programmed death-ligand 1 (PD-L1), acts as a ligand for programmed cell death protein 1 (PD-1). Though expressed primarily in dendritic cells, PD-L2 expression can be induced on a wide variety of immune and non-immune cells depending on the microenvironment. PD-L2 expression is particularly upregulated in the presence of Th2 cytokine, IL-4, as well as Th1 cytokines, TNF-alpha and IFN-gamma to a lesser degree. While generally expressed at lower levels compared to PD-L1, PD-L2 demonstrates a 2 to 6 times higher relative affinity to PD-1 than PD-L1. PD-1 and its ligands are referred to as inhibitory immune checkpoint molecules in that they provide useful negative feedback during physiological homeostasis. Ligation of PD-L2 or PD-L1 inhibits activation, proliferation, and cytokine secretion (e.g. IFN-gamma, IL-10) in T cells, ultimately dampening immune response. Conversely, studies have shown that PD-L2 can also stimulate T cell proliferation and cytokine production, even in PD-1-deficient T cells, suggesting additional receptors. Recent studies have concluded that PD-L2 also binds to a second receptor, repulsive guidance molecule b (RGMb), which was originally identified as a receptor for bone morphogenetic proteins (BMPs). RGMb is expressed in the central nervous system, as well as in macrophages, however, its role in immunity is only beginning to emerge. Interaction between PD-L2 and RGMb regulates the development of respiratory tolerance in the lung through BMP and/or neogenin signaling pathways. The naturally occurring human PD-L2 monomer consists of a 201-amino-acid extracellular domain, a 21-amino-acid transmembrane domain, and a 32-amino-acid cytoplasmic domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Dendritic cell Piezo1 directs the differentiation of T_H1 and T_reg cells in cancer.

eLife

Wang Y,Yang H,Jia A,Wang Y,Yang Q,Dong Y,Hou Y,Cao Y,Dong L,Bi Y,Liu G

12-5986-82 was used in Flow cytometry/Cell sorting to demonstrate the role of the dendritic cells-based mechanical regulation of immunopathology in directing T cell lineage commitment in tumor microenvironments.

Mon Aug 22 00:00:00 EDT 2022

Intestinal CD11b⁺ B Cells Ameliorate Colitis by Secreting Immunoglobulin A.

Frontiers in immunology

Fu Y,Wang Z,Yu B,Lin Y,Huang E,Liu R,Zhao C,Lu M,Xu W,Liu H,Liu Y,Wang L,Chu Y

12-5986-82 was used in Flow Cytometry to demonstrate that CD11b+ B cells are essential intestinal suppressive immune cells and the primary source of intestinal IgA, which plays an indispensable role in maintaining intestinal homeostasis.

Fri Jan 07 00:00:00 EST 2022

Liver Is a Generative Site for the B Cell Response to Ehrlichia muris.

Immunity

Trivedi N,Weisel F,Smita S,Joachim S,Kader M,Radhakrishnan A,Clouser C,Rosenfeld AM,Chikina M,Vigneault F,Hershberg U,Ismail N,Shlomchik MJ

12-5986-82 was used in Flow Cytometry to investigate the nature of liver B cell and spleen response to Ehrlichia muris.

Tue Dec 17 00:00:00 EST 2019

Regulatory T cells mediate specific suppression by depleting peptide-MHC class II from dendritic cells.

Nature immunology

Akkaya B,Oya Y,Akkaya M,Al Souz J,Holstein AH,Kamenyeva O,Kabat J,Matsumura R,Dorward DW,Glass DD,Shevach EM

12-5986-82 was used in Flow Cytometry to study whether pathways similar to the multiple suppressive mechansims triggered in vitro by T cell antigen receptor activated regulatory T cells exist in vivo.

Fri Feb 01 00:00:00 EST 2019

Glycolytic Stimulation Is Not a Requirement for M2 Macrophage Differentiation.

Cell metabolism

Wang F,Zhang S,Vuckovic I,Jeon R,Lerman A,Folmes CD,Dzeja PP,Herrmann J

12-5986-82 was used in flow cytometry to confirm he inhibitory effect of 2-DG on glycolysis, but also demonstrate that 2-DG impairs oxidative phosphorylation and significantly reduces 13C-labeled Krebs cycle metabolites and intracellular ATP levels to indicate that glycolytic stimulation is not required for M2 macrophage differentiation as long as oxidative phosphorylation remains active.

Tue Sep 04 00:00:00 EDT 2018

Recruited monocytes modulate malaria-induced lung injury through CD36-mediated clearance of sequestered infected erythrocytes.

Journal of leukocyte biology

Lagassé HA,Anidi IU,Craig JM,Limjunyawong N,Poupore AK,Mitzner W,Scott AL

Sun May 01 00:00:00 EDT 2016

Production of IL-10 by CD4(+) regulatory T cells during the resolution of infection promotes the maturation of memory CD8(+) T cells.

Nature immunology

Laidlaw BJ,Cui W,Amezquita RA,Gray SM,Guan T,Lu Y,Kobayashi Y,Flavell RA,Kleinstein SH,Craft J,Kaech SM

12-5986 was used in Flow cytometry/Cell sorting to that Treg cell-derived IL-10 is needed to insulate, and promote the quality of, developing CD8(+) T cells.

Sat Aug 01 00:00:00 EDT 2015

Haploinsufficiency for Stard7 is associated with enhanced allergic responses in lung and skin.

Journal of immunology (Baltimore, Md. : 1950)

Yang L,Lewkowich I,Apsley K,Fritz JM,Wills-Karp M,Weaver TE

12-5986 was used in Flow cytometry/Cell sorting to determine whether loss of Stard7 expression promotes allergic responses, showing that haploinsufficiency for Stard7 is associated with enhanced allergic responses.

Mon Jun 15 00:00:00 EDT 2015

Programmed death-1 pathway in host tissues ameliorates Th17/Th1-mediated experimental chronic graft-versus-host disease.

Journal of immunology (Baltimore, Md. : 1950)

Fujiwara H,Maeda Y,Kobayashi K,Nishimori H,Matsuoka K,Fujii N,Kondo E,Tanaka T,Chen L,Azuma M,Yagita H,Tanimoto M

12-5986 was used in Flow cytometry/Cell sorting to clarify that programed death-ligand 1 deficiency in host tissues, not hematopoietic cells, is associated with exacerbated chronic graft-versus-host disease.

Mon Sep 01 00:00:00 EDT 2014

Programmed death-1 pathway in host tissues ameliorates Th17/Th1-mediated experimental chronic graft-versus-host disease.

Journal of immunology (Baltimore, Md. : 1950)

Fujiwara H,Maeda Y,Kobayashi K,Nishimori H,Matsuoka K,Fujii N,Kondo E,Tanaka T,Chen L,Azuma M,Yagita H,Tanimoto M

Mon Sep 01 00:00:00 EDT 2014

Pneumolysin expression by streptococcus pneumoniae protects colonized mice from influenza virus-induced disease.

Virology

Wolf AI,Strauman MC,Mozdzanowska K,Williams KL,Osborne LC,Shen H,Liu Q,Garlick D,Artis D,Hensley SE,Caton AJ,Weiser JN,Erikson J

12-5986-82 was used in Flow Cytometry to investigate severity of influenza virus response with respiratory microbe presence.

Fri Aug 01 00:00:00 EDT 2014

PD-L2 modulates asthma severity by directly decreasing dendritic cell IL-12 production.

Mucosal immunology

Lewkowich IP,Lajoie S,Stoffers SL,Suzuki Y,Richgels PK,Dienger K,Sproles AA,Yagita H,Hamid Q,Wills-Karp M

12-5986 was used in Flow cytometry/Cell sorting to investigate the mechanism by which PD-L2 modulates asthma severity in human and mouse lung samples.

Mon Jul 01 00:00:00 EDT 2013

Th2 cell-intrinsic hypo-responsiveness determines susceptibility to helminth infection.

PLoS pathogens

van der Werf N,Redpath SA,Azuma M,Yagita H,Taylor MD

12-5986 was used in Flow cytometry/Cell sorting to demonstrate that during chronic Litomosoides sigmodontis infection, CD4(+) Th2 cells are conditioned towards an intrinsically hypo-responsive phenotype.

Fri Mar 01 00:00:00 EST 2013

Human and mouse macrophages collaborate with neutrophils to kill larval Strongyloides stercoralis.

Infection and immunity

Bonne-Année S,Kerepesi LA,Hess JA,O'Connell AE,Lok JB,Nolan TJ,Abraham D

12-5986 was used in Flow cytometry/Cell sorting to demonstrate a novel function by which alternatively activated macrophages control large extracellular parasites.

Sun Sep 01 00:00:00 EDT 2013

Properties of immature myeloid progenitors with nitric-oxide-dependent immunosuppressive activity isolated from bone marrow of tumor-free mice.

PloS one

Forghani P,Harris W,Giver CR,Mirshafiey A,Galipeau J,Waller EK

12-5986 was used in Flow cytometry/Cell sorting to determine the role of immature myeloid cells in regulating T-cell activation and proliferation in bone marrow.

Thu Jan 23 00:00:00 EST 2014

Programmed cell death 1 forms negative costimulatory microclusters that directly inhibit T cell receptor signaling by recruiting phosphatase SHP2.

The Journal of experimental medicine

Yokosuka T,Takamatsu M,Kobayashi-Imanishi W,Hashimoto-Tane A,Azuma M,Saito T

12-5986 was used in Flow cytometry/Cell sorting to investigate the molecular mechanisms of PD-1-mediated suppression of T cell activation, showing that it inhibits T cell receptor signalling via SHP2.

Mon Jun 04 00:00:00 EDT 2012

IL-10 controls dendritic cell-induced T-cell reactivation in the skin to limit contact hypersensitivity.

The Journal of allergy and clinical immunology

Girard-Madoux MJ,Kel JM,Reizis B,Clausen BE

12-5986 was used in Flow cytometry/Cell sorting to demonstrate that IL-10 signaling in dendritic cells is critical in preventing an exaggerated effector T-cell response in the skin.

Sun Jan 01 00:00:00 EST 2012

Tumor-initiated inflammation overrides protective adaptive immunity in an induced melanoma model in mice.

Cancer research

Soudja SM,Wehbe M,Mas A,Chasson L,de Tenbossche CP,Huijbers I,Van den Eynde B,Schmitt-Verhulst AM

12-5986 was used in Flow cytometry/Cell sorting to study the effect of the immune system on two differentially aggressive melanomas developing in mice on conditional deletion of the INK4A/ARF tumour suppressor gene.

Sat May 01 00:00:00 EDT 2010

Partial restoration of T-cell function in aged mice by in vitro blockade of the PD-1/ PD-L1 pathway.

Aging cell

Lages CS,Lewkowich I,Sproles A,Wills-Karp M,Chougnet C

12-5986 was used in Flow cytometry/Cell sorting to suggest that blockade of the PD-1/PD-L1 is not likely to be efficient at restoring exhausted T-cell responses in aged hosts.

Fri Oct 01 00:00:00 EDT 2010

PD-L1 and PD-L2 modulate airway inflammation and iNKT-cell-dependent airway hyperreactivity in opposing directions.

Mucosal immunology

Akbari O,Stock P,Singh AK,Lombardi V,Lee WL,Freeman GJ,Sharpe AH,Umetsu DT,Dekruyff RH

12-5986 was used in Flow cytometry/Cell sorting to investigate the roles of PD-L1 and PD-L2 in regulating invariant natural killer T cell mediated airway hyperreactivity in a mouse model of asthma.

Fri Jan 01 00:00:00 EST 2010

Costimulator B7-DC attenuates strong Th2 responses induced by Nippostrongylus brasiliensis.

Journal of immunology (Baltimore, Md. : 1950)

Ishiwata K,Watanabe N,Guo M,Tomihara K,Brumlik MJ,Yagita H,Pardoll D,Chen L,Shin T

12-5986 was used in Flow cytometry/Cell sorting to demonstrate that the inhibition of Th2 responses by B7-DC occurs independently of programmed death 1 but conceivably acts through an as yet unknown alternative receptor that enhances Th1 responses.

Mon Feb 15 00:00:00 EST 2010

Foxa2 programs Th2 cell-mediated innate immunity in the developing lung.

Journal of immunology (Baltimore, Md. : 1950)

Chen G,Wan H,Luo F,Zhang L,Xu Y,Lewkowich I,Wills-Karp M,Whitsett JA

12-5986 was used in Flow cytometry/Cell sorting to demonstrate that selective expression of Foxa2 plays a key role in regulating Th2 cell-mediated pulmonary inflammation.

Tue Jun 01 00:00:00 EDT 2010

Antigen-specific immunity and cross-priming by epithelial ovarian carcinoma-induced CD11b(+)Gr-1(+) cells.

Journal of immunology (Baltimore, Md. : 1950)

Tomihara K,Guo M,Shin T,Sun X,Ludwig SM,Brumlik MJ,Zhang B,Curiel TJ,Shin T

12-5986 was used in Flow cytometry/Cell sorting to investigate CD11b(+)Gr-1(+) cells found in epithelial ovarian cancer-bearing mice, which are induced by ovarian cancer cells and undergo antigen-specific immunity and cross-priming.

Tue Jun 01 00:00:00 EDT 2010

The herbal medicine compound falcarindiol from Notopterygii Rhizoma suppresses dendritic cell maturation.

The Journal of pharmacology and experimental therapeutics

Mitsui S,Torii K,Fukui H,Tsujimura K,Maeda A,Nose M,Nagatsu A,Mizukami H,Morita A

12-5986 was used in Flow cytometry/Cell sorting to study the effects of falcarindiol on dendritic cell function.

Tue Jun 01 00:00:00 EDT 2010

Allostimulatory activity of bone marrow-derived plasmacytoid dendritic cells is independent of indoleamine dioxygenase but regulated by inducible costimulator ligand expression.

Human immunology

Colvin BL,Sumpter TL,Tokita D,Salati J,Mellor AL,Thomson AW

12-5986 was used in Flow cytometry/Cell sorting to implicate inducible costimulator ligand-induced IL-10 in the regulation of bone marrow-derived plasmacytoid dendritic cell function.

Fri May 01 00:00:00 EDT 2009

A critical role of IFNgamma in priming MSC-mediated suppression of T cell proliferation through up-regulation of B7-H1.

Cell research

Sheng H,Wang Y,Jin Y,Zhang Q,Zhang Y,Wang L,Shen B,Yin S,Liu W,Cui L,Li N

12-5986 was used in Flow cytometry/Cell sorting to investigate the role that IFNγ plays in mesenchymal stem cell mediated suppression of T cell proliferation.

Fri Aug 01 00:00:00 EDT 2008

Identification of discrete tumor-induced myeloid-derived suppressor cell subpopulations with distinct T cell-suppressive activity.

Blood

Movahedi K,Guilliams M,Van den Bossche J,Van den Bergh R,Gysemans C,Beschin A,De Baetselier P,Van Ginderachter JA

12-5986 was used in Flow cytometry/Cell sorting to identify 2 distinct MDSC subfractions with clear morphologic, molecular, and functional differences.

Tue Apr 15 00:00:00 EDT 2008

Dynamics of lung macrophage activation in response to helminth infection.

Journal of leukocyte biology

Siracusa MC,Reece JJ,Urban JF,Scott AL

12-5986 was used in Flow cytometry/Cell sorting to study the dynamics of lung macrophage activation in response to helminth infection.

Mon Dec 01 00:00:00 EST 2008

In vivo costimulatory role of B7-DC in tuning T helper cell 1 and cytotoxic T lymphocyte responses.

The Journal of experimental medicine

Shin T,Yoshimura K,Shin T,Crafton EB,Tsuchiya H,Housseau F,Koseki H,Schulick RD,Chen L,Pardoll DM

12-5986 was used in Functional assays to investigate the in vivo roles of costimulatory/coinhibitory molecules B7-DC and B7-H1.

Mon May 16 00:00:00 EDT 2005

Migratory Langerhans cells in mouse lymph nodes in steady state and inflammation.

The Journal of investigative dermatology

Stoitzner P,Tripp CH,Douillard P,Saeland S,Romani N

12-5986 was used in Flow cytometry/Cell sorting to determine that the overall phenotype of Langerhans cells is not an indicator for their immunogenic or tolerogenic potential.

Fri Jul 01 00:00:00 EDT 2005

Essential role of lung plasmacytoid dendritic cells in preventing asthmatic reactions to harmless inhaled antigen.

The Journal of experimental medicine

de Heer HJ,Hammad H,Soullié T,Hijdra D,Vos N,Willart MA,Hoogsteden HC,Lambrecht BN

12-5986 was used in Flow cytometry/Cell sorting to investigate the role of lung plasmacytoid dendritic cells in atopic asthma.

Mon Jul 05 00:00:00 EDT 2004

Cooperative B7-1/2 (CD80/CD86) and B7-DC costimulation of CD4+ T cells independent of the PD-1 receptor.

The Journal of experimental medicine

Shin T,Kennedy G,Gorski K,Tsuchiya H,Koseki H,Azuma M,Yagita H,Chen L,Powell J,Pardoll D,Housseau F

12-5986 was used in Flow cytometry/Cell sorting to show costimulation with B7-DC alone or in conjunction with B7-1 is PD-1 independent, indicating that B7-DC costimulates T cells via a second receptor.

Mon Jul 07 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: B7-DC; Butyrophilin B7-DC; butyrophilin-like protein; CD273; MGC124039; MGC124040; PD-1 ligand 2; PD1 ligand 2; PDCD1 ligand 2; Programmed cell death 1 ligand 2; programmed death ligand 2

View more View less

Gene Aliases: B7-DC; B7dc; Btdc; Cd273; F730015O22Rik; PD-L2; Pdcd1lg2; Pdl2

View more View less

UniProt ID: (Mouse) Q9WUL5

View more View less

Entrez Gene ID: (Mouse) 58205

View more View less

Function(s)

protein binding immunoglobulin receptor superfamily

Process(es)

negative regulation of interferon-gamma production negative regulation of interleukin-10 production positive regulation of T cell proliferation negative regulation of T cell proliferation negative regulation of activated T cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD273 (B7-DC) Monoclonal Antibody (TY25), PE, eBioscience™

CD273 (B7-DC) Antibody (12-5986-82) in Flow

Product Details

12-5986-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!