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Invitrogen
Granulysin Monoclonal Antibody (eBioDH2 (DH2)), PE, eBioscience™
FIGURE: 1 / 5
Granulysin Antibody (12-8828-42) in Flow
Normal human peripheral blood cells were stained with CD56 Monoclonal Antibody, APC (Product # 17-0567-42) and Mouse IgG1 kappa Isotype Control, PE (Product # 12-4714-82) (left) or Granulysin Monoclonal Antibody, PE (right). Cells in the lymphocyte gate were used for analysis.
Product Details
12-8828-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Rhesus monkey
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBioDH2 (DH2)
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10854120
Product Specific Information
Description: The eBioDH2 monoclonal antibody reacts with human granulysin, a protein involved in the cytolytic pathway. Granulysin is a member of the saposin-like protein (SAPLIP) family. Two proteins 9 and 15kDa are thought to be post translation modified versions of the 15kDa protein with a change in confirmation. Granulysin is found in NK and activated T cells and the expression is confined to the cytolytic granules which also contains perforin and granzyme. Granulysin with perforn are suspected to function synergistically to kill bacteria and induce apoptosis in nucleated cells.
Applications Reported: This eBioDH2 (DH2) antibody has been reported for use in intracellular staining followed by flow cytometric analysis and immunocytochemistry.
Applications Tested: This eBioDH2 (DH2) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
GNLY is a member of the saposin-like protein (SAPLIP) family and is located in the cytotoxic granules of T cells, which are released upon antigen stimulation. GNLY is present in cytotoxic granules of cytotoxic T lymphocytes and natural killer cells, and it has antimicrobial activity against M. tuberculosis and other organisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Granulysin; lymphocyte-activation gene 2; Lymphokine; Lymphokine LAG-2; Lymphokine NKG5519; Protein NKG5; T-cell activation protein 519; T-lymphocyte activation gene 519
Gene Aliases: D2S69E; GNLY; LAG-2; LAG2; NKG5; TLA519
UniProt ID: (Human) P22749
Entrez Gene ID: (Human) 10578
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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