Invitrogen

TRA-1-60 (Podocalyxin) Monoclonal Antibody (TRA-1-60), PE, eBioscience™

3 Published Figures

12 References

View all (11) TRA-1-60 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (3)

TRA-1-60 (Podocalyxin) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 4

TRA-1-60 (Podocalyxin) Antibody (12-8863-82) in Flow

Staining of 2102Ep human embryonal carcinoma cell line with 0.5 µg of Mouse IgM Isotype Control PE (Product # 12-4752) (open histogram) or 0.5 µg of Anti-Human TRA-1-60 PE (filled histogram). Total viable cells were used for analysis.

Product Image Gallery

Product Details

12-8863-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 11 publications 11 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

TRA-1-60

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_891602

Product Specific Information

Description: The TRA-1-60 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embyronal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-60 antibody is lost upon cell differentiation. Contrary to early reports that the TRA-1-60 epitope can be destroyed by neuraminidase digestion, new strudies have shown that TRA-1-60 recognizes a neurominidase resistent antigen. The TRA-1-60 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.

Applications Reported: This TRA-1-60 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TRA-1-60 antibody has been tested by flow cytometric analysis of the human embryonal carcinoma (EC) line 2102Ep. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Selective vulnerability of human-induced pluripotent stem cells to dihydroorotate dehydrogenase inhibition during mesenchymal stem/stromal cell purification.

Frontiers in cell and developmental biology

Al-Akashi Z,Zujur D,Kamiya D,Kato T,Kondo T,Ikeya M

12-8863-82 was used in Flow cytometry/Cell sorting to demonstrates the Brequinar (BRQ)-induced inhibition of dihydroorotate dehydrogenase (DHODH), a key enzyme in de novo pyrimidine biosynthesis, selectively induces apoptosis, cell cycle arrest, and differentiation; furthermore, it promotes transcriptional changes and prevents the growth of 3-dimensional hiPSC aggregates.

Fri Feb 24 00:00:00 EST 2023

Characterization of intracellular buffering power in human induced pluripotent stem cells and the loss of pluripotency is delayed by acidic stimulation and increase of NHE1 activity.

Journal of cellular physiology

Lee SP,Chao SC,Chou MF,Huang SF,Dai NT,Wu GJ,Tsai CS,Loh SH,Tsai YT

12-8863-82 was used in Flow cytometry/Cell sorting to establish a quantitative model of passive β during differentiation and demonstrate that maintenance of NHE1 at a higher level is of critical importance for pluripotency retention in hiPSCs.

Mon Feb 01 00:00:00 EST 2021

Ultra-High-Frequency Reprogramming of Individual Long-Term Hematopoietic Stem Cells Yields Low Somatic Variant Induced Pluripotent Stem Cells.

Cell reports

Wang K,Guzman AK,Yan Z,Zhang S,Hu MY,Hamaneh MB,Yu YK,Tolu S,Zhang J,Kanavy HE,Ye K,Bartholdy B,Bouhassira EE

12-8863-82 was used in Flow Cytometry to suggest LT-HSCs may become the preferred cell source for the production of clinical-grade iPSCs.

Tue Mar 05 00:00:00 EST 2019

Analysis of Transcriptional Variability in a Large Human iPSC Library Reveals Genetic and Non-genetic Determinants of Heterogeneity.

Cell stem cell

Carcamo-Orive I,Hoffman GE,Cundiff P,Beckmann ND,D'Souza SL,Knowles JW,Patel A,Papatsenko D,Abbasi F,Reaven GM,Whalen S,Lee P,Shahbazi M,Henrion MYR,Zhu K,Wang S,Roussos P,Schadt EE,Pandey G,Chang R,Quertermous T,Lemischka I

12-8863 was used in Immunocytochemistry to examine the sources of gene expression variability in 317 human induced pluripotent stem cell lines from 101 individuals.

Thu Apr 06 00:00:00 EDT 2017

Comparison of Four Protocols to Generate Chondrocyte-Like Cells from Human Induced Pluripotent Stem Cells (hiPSCs).

Stem cell reviews and reports

Suchorska WM,Augustyniak E,Richter M,Trzeciak T

Published figure using TRA-1-60 (Podocalyxin) monoclonal antibody (Product # 12-8863-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

TRIM28 and Interacting KRAB-ZNFs Control Self-Renewal of Human Pluripotent Stem Cells through Epigenetic Repression of Pro-differentiation Genes.

Stem cell reports

Oleksiewicz U,Gładych M,Raman AT,Heyn H,Mereu E,Chlebanowska P,Andrzejewska A,Sozańska B,Samant N,Fąk K,Auguścik P,Kosiński M,Wróblewska JP,Tomczak K,Kulcenty K,Płoski R,Biecek P,Esteller M,Shah PK,Rai K,Wiznerowicz M

Published figure using TRA-1-60 (Podocalyxin) monoclonal antibody (Product # 12-8863-82) in Flow Cytometry

Tue Dec 12 00:00:00 EST 2017

A new class of temporarily phenotypic enhancers identified by CRISPR/Cas9-mediated genetic screening.

Genome research

Diao Y,Li B,Meng Z,Jung I,Lee AY,Dixon J,Maliskova L,Guan KL,Shen Y,Ren B

12-8863 was used in Flow cytometry/Cell sorting to demonstrate the utility of high-throughput screening for noncoding DNA functional characterisation and reveal a novel layer of gene regulation in humans.

Tue Mar 01 00:00:00 EST 2016

Toward Personalized Medicine: Using Cardiomyocytes Differentiated From Urine-Derived Pluripotent Stem Cells to Recapitulate Electrophysiological Characteristics of Type 2 Long QT Syndrome.

Journal of the American Heart Association

Jouni M,Si-Tayeb K,Es-Salah-Lamoureux Z,Latypova X,Champon B,Caillaud A,Rungoat A,Charpentier F,Loussouarn G,Baró I,Zibara K,Lemarchand P,Gaborit N

12-8863 was used in Flow cytometry/Cell sorting to evaluate the use of cardiomyocytes differentiated from urine-derived stem cells as a new cellular model for studying patients' specific arrhythmia mechanisms.

Tue Sep 01 00:00:00 EDT 2015

Autophagy regulates homeostasis of pluripotency-associated proteins in hESCs.

Stem cells (Dayton, Ohio)

Cho YH,Han KM,Kim D,Lee J,Lee SH,Choi KW,Kim J,Han YM

12-8863 was used in Flow cytometry/Cell sorting to demonstrate that autophagy acts together with the ubiquitin-proteasome system to modulate the levels of pluripotency-associated proteins in human embryonic stem cells.

Sat Feb 01 00:00:00 EST 2014

PRMT5 is required for human embryonic stem cell proliferation but not pluripotency.

Stem cell reviews and reports

Gkountela S,Li Z,Chin CJ,Lee SA,Clark AT

Published figure using TRA-1-60 (Podocalyxin) monoclonal antibody (Product # 12-8863-82) in Flow Cytometry

Tue Apr 01 00:00:00 EDT 2014

Few single nucleotide variations in exomes of human cord blood induced pluripotent stem cells.

PloS one

Su RJ,Yang Y,Neises A,Payne KJ,Wang J,Viswanathan K,Wakeland EK,Fang X,Zhang XB

12-8863 was used in Flow cytometry/Cell sorting to study the effect of the cellular reprogramming process on mutation load in induced pluripotent stem cells.

Tue Oct 22 00:00:00 EDT 2013

Procedures for derivation and characterisation of human embryonic stem cells from Odense, Denmark.

Methods in molecular biology (Clifton, N.J.)

Harkness L,Kassem M

12-8863-82 was used in flow cytometry to describe the derivation methods used by our laboratory for isolation of the ICM by immunosurgery and outgrowth of the whole blastocyst.

Fri Aug 10 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: TRA160; tra60

View more View less

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

TRA-1-60 (Podocalyxin) Monoclonal Antibody (TRA-1-60), PE, eBioscience™

TRA-1-60 (Podocalyxin) Antibody (12-8863-82) in Flow

Product Details

12-8863-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!