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Description: Granzyme M is one of five granzyme serine proteases that have been identified in humans. These proteins are expressed in the granules of NK cells and cytotoxic T cells, and are critical for the induction of target cell apoptosis through the cleavage of intracellular substrates. Although not as well characterized as some of the other family members, Granzyme M has been found to exhibit some distinct functions. These include the non-cytotoxic inhibition of cytomegalovirus replication, induction of apoptosis in tumor cells, and contribution to the TLR4-induced inflammatory process.
Applications Reported: This 4B2G4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 4B2G4 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located at Flow Protocols . This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Cleaves peptide substrates after methionine, leucine, and norleucine. Physiological substrates include EZR, alpha- tubulins and the apoptosis inhibitor BIRC5/Survivin. Promotes caspase activation and subsequent apoptosis of target cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Granzyme M; granzyme M (lymphocyte met-ase 1); HU-Met-1; lymphocyte met-ase 1; Met-1 serine protease; Met-ase; Natural killer cell granular protease
Gene Aliases: GZMM; LMET1; MET1
UniProt ID: (Human) P51124
Entrez Gene ID: (Human) 3004
Molecular Function: serine protease
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