Invitrogen

c-MAF Monoclonal Antibody (sym0F1), PE, eBioscience™

5 Published Figures

9 References

View all (38) c-MAF antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (5)

FIGURE: 1 / 6

c-MAF Antibody (12-9855-42) in Flow

Staining of 3-day Th17-polarized mouse splenocytes with Anti-Mouse CD4 FITC (Product # 11-0042-82) and Mouse IgG2b K Isotype Control PE (Product # 12-4732-81) (left) or Anti-Human/Mouse c-Maf PE (right). Viable cells in the lymphocyte gate, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866-14), were used for analysis.

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Product Details

12-9855-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

sym0F1

Immunogen

Full-length E. coli-produced c-Maf

View immunogen

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572747

Product Specific Information

Description: The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor shown to be involved in the neural, ocular and hematopoietic systems. In hematopoietic cells, it was first shown to be crucial for IL-4 expression in Th2 and was the first transcription factor believed to be Th subset-specific. More recent evidence shows that specific phospho-tyrosine residues lead to upregulation of IL-4. c-Maf has also been shown to be important to differentiation and function in both Th17 and Tfh cells. It drives expression of IL-21 in both cell types, while promoting expression of IL-23R in Th17 and CXCR5 in Tfh as well.

Sym0F1 does not cross-react with MafA or MafB.

Applications Reported: This sym0F1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This sym0F1 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of Th17-polarized mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

c-maf is the cellular counterpart of oncogenic v-maf that belongs to the family of basic region leucine zipper domain transcription factors. The leucine-zipper domain is involved in the interaction with LRPICD. There are two forms of human c-maf mRNA, c-maf-long and c-maf-short. It is identified in the genome of the acute transforming avian retrovirus AS42. c-maf targets are IL-4 in Th2 cells, the crystalline genes in lens fiber cells, insulin gene in islet, p53 and L7 where it exerts its transcriptional role through binding to a Maf recognition element (MARE). It regulates Th2 differentiation and lineage-specific hematopoiesis. c-maf is a transcription factor for IL-10 gene expression in LPS-activated macrophages. Chromosomal aberration involving maf is found in some forms of multiple myeloma. It is expressed in myeloma cell lines and resting monocytes/macrophages.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Microbiota-assisted iron uptake promotes immune tolerance in the intestine.

Nature communications

Zhu L,Li G,Liang Z,Qi T,Deng K,Yu J,Peng Y,Zheng J,Song Y,Chang X

Published figure using c-MAF monoclonal antibody (Product # 12-9855-42) in Flow Cytometry

Mon May 15 00:00:00 EDT 2023

Natural γδT17 cell development and functional acquisition is governed by the mTORC2-c-Maf-controlled mitochondrial fission pathway.

iScience

Wang Y,Qin H,Cai Y,Chen X,Li H,Montoya-Durango DE,Ding C,Hu X,Chariker JH,Sarojini H,Chien S,Rouchka EC,Zhang HG,Zheng J,Qiu F,Yan J

12-9855-42 was used in Flow cytometry/Cell sorting to provide an in-depth insight into the intrinsic link between the mitochondrial OXPHOS pathway and γδT17 thymic programming and functional acquisition.

Fri May 19 00:00:00 EDT 2023

GM-CSF-activated human dendritic cells promote type 1 T follicular helper cell polarization in a CD40-dependent manner.

Journal of cell science

Korniotis S,Saichi M,Trichot C,Hoffmann C,Amblard E,Viguier A,Grondin S,Noel F,Mattoo H,Soumelis V

Published figure using c-MAF monoclonal antibody (Product # 12-9855-42) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

GM-CSF-activated human dendritic cells promote type 1 T follicular helper cell polarization in a CD40-dependent manner.

Journal of cell science

Korniotis S,Saichi M,Trichot C,Hoffmann C,Amblard E,Viguier A,Grondin S,Noel F,Mattoo H,Soumelis V

Published figure using c-MAF monoclonal antibody (Product # 12-9855-42) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

Oncogenic Vav1-Myo1f induces therapeutically targetable macrophage-rich tumor microenvironment in peripheral T cell lymphoma.

Cell reports

Cortes JR,Filip I,Albero R,Patiño-Galindo JA,Quinn SA,Lin WW,Laurent AP,Shih BB,Brown JA,Cooke AJ,Mackey A,Einson J,Zairis S,Rivas-Delgado A,Laginestra MA,Pileri S,Campo E,Bhagat G,Ferrando AA,Rabadan R,Palomero T

12-9855-42 was used in Flow Cytometry /test) to study the specific role and mechanism of peripheral T cell lymphoma not otherwise specified (PTCL-NOS).

Tue Apr 19 00:00:00 EDT 2022

Transcriptome dynamics of CD4⁺ T cells during malaria maps gradual transit from effector to memory.

Nature immunology

Soon MSF,Lee HJ,Engel JA,Straube J,Thomas BS,Pernold CPS,Clarke LS,Laohamonthonkul P,Haldar RN,Williams CG,Lansink LIM,Moreira ML,Bramhall M,Koufariotis LT,Wood S,Chen X,James KR,Lönnberg T,Lane SW,Belz GT,Engwerda CR,Khoury DS,Davenport MP,Svensson V,Teichmann SA,Haque A

12-9855 was used in Flow cytometry/Cell sorting to apply single-cell RNA sequencing and computational modelling to track memory development during Plasmodium infection and treatment.

Tue Dec 01 00:00:00 EST 2020

C5aR1 regulates T follicular helper differentiation and chronic graft-versus-host disease bronchiolitis obliterans.

JCI insight

Verghese DA,Chun N,Paz K,Fribourg M,Woodruff TM,Flynn R,Hu Y,Xiong H,Zhang W,Yi Z,Du J,Blazar BR,Heeger PS

Published figure using c-MAF monoclonal antibody (Product # 12-9855-42) in Flow Cytometry

Thu Dec 20 00:00:00 EST 2018

Epidermal Growth Factor Receptor Expression Licenses Type-2 Helper T Cells to Function in a T Cell Receptor-Independent Fashion.

Immunity

Minutti CM,Drube S,Blair N,Schwartz C,McCrae JC,McKenzie AN,Kamradt T,Mokry M,Coffer PJ,Sibilia M,Sijts AJ,Fallon PG,Maizels RM,Zaiss DM

12-9855 was used in Flow cytometry/Cell sorting to reveal a mechanism by which antigen presentation controls the innate effector function of Th2 cells at the site of inflammation.

Tue Oct 17 00:00:00 EDT 2017

Characterization of c-Maf⁺Foxp3^- Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells.

Scientific reports

Chien CH,Yu HC,Chen SY,Chiang BL

12985542 was used in flow cytometry to generate and characterize Treg-of-B cells

Wed Apr 12 00:00:00 EDT 2017

Human circulating PD-1+CXCR3-CXCR5+ memory Tfh cells are highly functional and correlate with broadly neutralizing HIV antibody responses.

Immunity

Locci M,Havenar-Daughton C,Landais E,Wu J,Kroenke MA,Arlehamn CL,Su LF,Cubas R,Davis MM,Sette A,Haddad EK,Poignard P,Crotty S

Thu Oct 17 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene; c-Maf long form; c-maf proto-oncogene; MGC71685; Proto-oncogene c-Maf; T lymphocyte c-maf long form; Transcription factor Maf; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog; V-maf musculoaponeurotic fibrosarcoma oncogene homolog

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Gene Aliases: 2810401A20Rik; A230108G15Rik; AW047063; AYGRP; c-MAF; CCA4; CTRCT21; MAF; Maf2

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UniProt ID: (Human) O75444, (Mouse) P54843

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Entrez Gene ID: (Human) 4094, (Mouse) 17132

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Function(s)

transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding protein binding sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding DNA binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding protein homodimerization activity protein heterodimerization activity basic leucine zipper transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter cytokine production regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter regulation of chondrocyte differentiation positive regulation of transcription from RNA polymerase II promoter cell development inner ear development lens fiber cell differentiation lens development in camera-type eye transcription, DNA-templated regulation of transcription, DNA-templated positive regulation of gene expression

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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c-MAF Monoclonal Antibody (sym0F1), PE, eBioscience™

c-MAF Antibody (12-9855-42) in Flow

Product Details

12-9855-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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