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BATF Antibody (12-9860-42) in Flow
Intracellular staining of normal human peripheral blood cells either unstimulated (orange historgam) or stimulated for 3 days with Anti-Human CD3 (Product # 16-0037-81) and Anti-Human CD28 (Product # 16-0289-81) (purple histogram) with Anti-Human BATF PE using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Cells in the the lymphocyte gate were used for analysis.
Product Details
12-9860-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MBM7C7
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11043266
Product Specific Information
Description: The MBM7C7 monoclonal antibody reacts with human and mouse BATF (B cell activating transcription factor). BATF is a 14 kDa member of the AP-1 family of transcription factors and is involved in the repression of other AP-1 family members. BATF is upregulated in activated T cells when compared to naive T and B cells and has been shown to be critical for Th17, but not Th1 or Th2 differentiation. In Th17, BATF interacts with the promoters of IL17, IL17F, IL21 and IL22. BATF has also been shown to be involved in CD8 T cell exhaustion in HIV infection.
Applications Reported: This MBM7C7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This MBM7C7 antibody has been pre-titrated and tested in intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
BATF is a nuclear basic leucine zipper protein that belongs to the AP-1/ATF superfamily of transcription factors. The leucine zipper of this protein mediates dimerization with members of the Jun family of proteins. This protein is thought to be a negative regulator of AP-1/ATF transcriptional events and blocks cellular transformation by Ras and Fos. BATF also is required for the differentiation of IL-17-producing T helper (TH17) cells, a CD4+ subset of T cells that coordinates the inflammatory response in host defense. In both T cells and B cells, BATF is required for the appropriate regulation of the class-switch recombination.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: activating transcription factor B; B-ATF; B-cell-activating transcription factor; basic leucine zipper transcription factor, ATF-like; Basic leucine zipper transcriptional factor ATF-like; SF-HT-activated gene 2 protein; SFA-2
Gene Aliases: B-ATF; BATF; BATF1; SFA-2; SFA2
UniProt ID: (Human) Q16520
Entrez Gene ID: (Human) 10538
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific DNA binding
basic leucine zipper transcription factor
cytokine production
lymphoid progenitor cell differentiation
transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
DNA damage response, signal transduction by p53 class mediator
defense response to protozoan
myeloid dendritic cell differentiation
T-helper 2 cell differentiation
isotype switching
positive regulation of transcription from RNA polymerase II promoter
hematopoietic stem cell differentiation
T-helper 17 cell differentiation
T-helper 17 cell lineage commitment
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