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Invitrogen
PAX5 Monoclonal Antibody (1H9), PE, eBioscience™
FIGURE: 1 / 2
PAX5 Antibody (12-9918-80) in Flow
Surface staining of C57BL/6 splenocytes with Anti-Mouse CD23 PE-Cy7 (Product # 25-0232-82) and Anti-Mouse CD21/CD35 FITC (Product # 11-0211-82) followed by intracellular staining using the Foxp3 Staining Buffer Set (Product # 00-5523-00) and 0.125 µg of Anti-Human/Mouse Pax5 PE. Histogram depicts cell populations based on surface stains comprising the marginal zone cells (CD21 (high)CD23-) (blue histogram) or T1 cells (CD21-CD23-) (green histogram).
Product Details
12-9918-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
1H9
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1944473
Product Specific Information
Description: The monoclonal antibody 1H9 recognizes both mouse and human Pax5. Pax5, also known as BSAP (B cell specific activator protein), is a member of the paired box (pax) family of transcription factors. Pax5 is the only member of the pax family of transcription factors that is expressed in hematopoietic cells. During embryogenesis, Pax5 is transiently expressed in the brain of mice and in the mesencephalon and spinal cord of humans. Its expression is upregulated early in B cell development at the time of B cell commitment and is maintained throughout most subsequent stages. Suppression of Pax5 is essential for expression of Blimp-1 and the terminal differentiation of plasma cells. In the spleen, expression of Pax5 is higher in marginal zone B cells (B220+ CD21high CD23low) than in other B cells, especially the transition 1 stage (B220+ CD21- CD23-). In addition to its role in B cell development, Pax5 also affects VH-DJH heavy chain recombination as well as influencing the expression of many other B and non-B cell related proteins. Pax5 expression is correlated with many neoplasms. In diffuse large B cell lymphomas (DLBCL) and non-Hodgkin lymphomas Pax5 is often mutated while in B-cell ALL, expression levels are high. Additionally, translocation with elastin, IGH, ETV6, FOXP1, and EVI3 have been identified.
eBioscience recommends that the Foxp3 Buffers (Product # 00-5521) be used for optimal results when using this antibody for intracellular staining and flow cytometric analysis.
Applications Reported: This 1H9 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 1H9 antibody has been tested by intracellular staining and flow cytometric analysis using the Foxp3/Transcription Factor Staining Buffer Set () and protocol. Please refer to Best Protocols: Protocol B: One-step protocol for intracellular (nuclear) proteins. This antibody can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
PAX5 is a member of the paired box (PAX) family of transcription factors. The PAX proteins are important regulators in early development and alterations in the expression of their genes are thought to contribute to neoplastic transformation. PAX5 is the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages, of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis. Mutations in the gene can result in leukemia, acute lymphoblastic 3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-cell lineage specific activator; B-cell-specific transcription factor; BSAP; paired box gene 5; paired box homeotic gene 5; Paired box protein Pax-5; paired domain gene 5; Pax5 variant 1; Pax5 variant 2; transcription factor PAX 5
Gene Aliases: ALL3; BSAP; EBB-1; KLP; Pax-5; PAX5
UniProt ID: (Human) Q02548, (Mouse) Q02650
Entrez Gene ID: (Human) 5079, (Mouse) 18507
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
multicellular organism development
spermatogenesis
nervous system development
aging
organ morphogenesis
lateral ventricle development
cerebral cortex development
cell differentiation
adult behavior
skeletal muscle cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
embryonic cranial skeleton morphogenesis
negative regulation of histone H3-K9 methylation
humoral immune response
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