Invitrogen

HLA-G Monoclonal Antibody (87G), PE, eBioscience™

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Antibody Testing Data (1)
Published Figures (3)

FIGURE: 1 / 4

HLA-G Antibody (12-9957-42) in Flow

Staining of GM-CSF and IFN gamma-stimulated U937 cells with Mouse IgG2a kappa Isotype Control PE (Product # 12-4724-81) (blue histogram) or Anti-Human HLA-G PE (purple histogram). Unstimulated U937 cells stained with Anti-Human HLA-G PE are shown in the green histogram. Total viable cells were used for analysis.

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Product Details

12-9957-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

87G

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11149313

Product Specific Information

Description: The monoclonal antibody 87G recognizes human HLA-G, a member of the Human Leukocyte Antigen family but as part of the nonclassical MHC type involved in inhibiting immune reponses. HLA-G has seven reported isoforms. The antibody 87G recognizes both HLA-G1 and the solube HLA-G5. Expression of HLA-G is found primarily in fetal trophoblast cells as they invade the maternal decidua thereby protecting the fetus from the maternal immune system. Like the highly mitotic trophoblast, abundant HLA-G protein expression has been identified in some tumors, including melanoma, breast carcinoma and renal carcinoma as well as CLL, AML and B-CLL. Some expression has also been found in pancreatic islets, erythroid and endothelial progenitors and the adult thymic medulla.HLA-G+ CD4 or CD8 cells have been identified in normal human peripheral blood and are thought to act as regulatory cells in that they are hypoproliferative with a unique cytokine profile differing from Tregs. The receptors for HLA-G are CD85j/ILT2, CD85d/ILT4, and CD158. Recent studies have shown a role for HLA-G in tolerance and maintenance of transplanted organs.

Applications Reported: This 87G antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 87G antibody has been pre-titrated and tested by flow cytometric analysis of stimulated U937 cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

CAR-T cells targeting HLA-G as potent therapeutic strategy for EGFR-mutated and overexpressed oral cancer.

iScience

Lin YC,Hua CH,Lu HM,Huang SW,Chen Y,Tsai MH,Lin FY,Canoll P,Chiu SC,Huang WH,Cho DY,Jan CI

12-9957-42 was used in Flow cytometry/Cell sorting to show that the upregulation of NLRP3 inflammasome leads to abundant HLA-G in the cytoplasm and cell membrane of FaDu cells.

Fri Mar 17 00:00:00 EDT 2023

Placental endovascular extravillous trophoblasts (enEVTs) educate maternal T-cell differentiation along the maternal-placental circulation.

Cell proliferation

Ma Y,Yang Q,Fan M,Zhang L,Gu Y,Jia W,Li Z,Wang F,Li YX,Wang J,Li R,Shao X,Wang YL

12-9957 was used in Flow cytometry/Cell sorting to study the TGF-β1 expression and distribution of regulatory T cells (Tregs) along the placental-maternal circulation route.

Fri May 01 00:00:00 EDT 2020

Placental endovascular extravillous trophoblasts (enEVTs) educate maternal T-cell differentiation along the maternal-placental circulation.

Cell proliferation

Ma Y,Yang Q,Fan M,Zhang L,Gu Y,Jia W,Li Z,Wang F,Li YX,Wang J,Li R,Shao X,Wang YL

12-9957 was used in Flow cytometry/Cell sorting to study the TGF-β1 expression and distribution of regulatory T cells (Tregs) along the placental-maternal circulation route.

Fri May 01 00:00:00 EDT 2020

hESC-derived immune suppressive dendritic cells induce immune tolerance of parental hESC-derived allografts.

EBioMedicine

Todorova D,Zhang Y,Chen Q,Liu J,He J,Fu X,Xu Y

12-9957 was used in Flow cytometry/Cell sorting to investigate whether the adoptive transfer of CTLA4-Ig/PD-L1 knock-in dendritic cell-like cells(CP DCLs) can induce long-term immune tolerance of parental human embryonic stem cells(hESC)-derived smooth muscle and cardiomyocyte allografts.

Tue Dec 01 00:00:00 EST 2020

Telomere length is positively associated with the expression of IL‑6 and MIP‑1α in bone marrow mesenchymal stem cells of multiple myeloma.

Molecular medicine reports

Li S,Jiang Y,Li A,Liu X,Xing X,Guo Y,Xu Y,Hao Y,Zheng C

12-9957-42 was used in Flow cytometry/Cell sorting to indicate that myeloma‚associated elongation of telomere length of bone marrow mesenchymal stem cells may be a key element contributing to the increased IL‚6 and MIP‚1α expression, by which MSCs in the tumor microenvironment may facilitate MM and/or MM bone disease development.

Fri Sep 01 00:00:00 EDT 2017

Feasibility of placenta-derived mesenchymal stem cells as a tool for studying pregnancy-related disorders.

Scientific reports

Fuchi N,Miura K,Doi H,Li TS,Masuzaki H

12-9957 was used in Flow cytometry/Cell sorting to evaluate the feasibility of using placenta-derived mesenchymal stem cells as a tool to study pregnancy related disorders.

Wed Apr 12 00:00:00 EDT 2017

ADAM12-directed ectodomain shedding of E-cadherin potentiates trophoblast fusion.

Cell death and differentiation

Aghababaei M,Hogg K,Perdu S,Robinson WP,Beristain AG

12-9957 was used in Flow cytometry/Cell sorting to identify E-cadherin as a novel A Disintegrin and Metalloproteinase 12 (ADAM12) substrate.

Tue Dec 01 00:00:00 EST 2015

L3MBTL1 deficiency directs the differentiation of human embryonic stem cells toward trophectoderm.

Stem cells and development

Hoya-Arias R,Tomishima M,Perna F,Voza F,Nimer SD

Published figure using HLA-G monoclonal antibody (Product # 12-9957-42) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2011

Homotypic aggregation of human cell lines by HLA class II-, class Ia- and HLA-G-specific monoclonal antibodies.

European journal of immunology

Odum N,Ledbetter JA,Martin P,Geraghty D,Tsu T,Hansen JA,Gladstone P

Sun Sep 01 00:00:00 EDT 1991

Bioinformatics

Protein Aliases: b2 microglobulin; DADB-15K14.8; HLA class I histocompatibility antigen, alpha chain G; HLA class I molecule; HLA G antigen; HLA-G histocompatibility antigen, class I, G; MHC class I antigen G

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Gene Aliases: HLA-6.0; HLA-G; HLAG; MHC-G

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UniProt ID: (Human) P17693

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Entrez Gene ID: (Human) 3135

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Function(s)

antigen binding receptor binding peptide antigen binding protein homodimerization activity major histocompatibility complex protein

Process(es)

antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent positive regulation of tolerance induction positive regulation of T cell tolerance induction immune response-inhibiting cell surface receptor signaling pathway cellular defense response antigen processing and presentation positive regulation of interleukin-12 production negative regulation of T cell proliferation positive regulation of regulatory T cell differentiation regulation of immune response negative regulation of immune response interferon-gamma-mediated signaling pathway type I interferon signaling pathway negative regulation of dendritic cell differentiation

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HLA-G Monoclonal Antibody (87G), PE, eBioscience™

HLA-G Antibody (12-9957-42) in Flow

Product Details

12-9957-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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