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Perforin Monoclonal Antibody (dG9 (delta G9)), PE, eBioscience™

7 Published Figures

18 References

View all (50) Perforin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (7)

Perforin Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 8

Perforin Antibody (12-9994-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD8 APC-eFluor® 780 (Product # 47-0088-42) followed by fixation and permeabilization with the Foxp3 Staining Buffers (Product # 00-5523-00) and intracellular staining with Mouse IgG2b K Isotype Control PE (Product # 12-4732-81) (left) or Anti-Human Perforin PE (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

12-9994-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 18 publications 18 publications

Product Specifications

Species Reactivity

Human, Pig

Published species

Human, Pig

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

dG9 (delta G9)

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10854416

Product Specific Information

Description: The dG9 antibody clone reacts with human perforin (pore-forming protein, pfp). Perforin is one of the cytolytic mediators present in the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections.

Applications Reported: This dG9 (delta G9) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This dG9 (delta G9) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Perforin is one of the major cytolytic proteins of cytolytic granules. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Moreover, perforin is involved in immune defense against tumors and virus infections as mediated by cytotoxic lymphocytes. Perforin is a 555 amino acid protein with a 21 amino acid signal peptide, and has a molecular weight of 70 to 75 kD. Perforin is a pore forming protein with a mechanism of transmembrane channel formation similar to C9, and homology between perforin and C9 have been demonstrated. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested. Perforin is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in the perforin gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants of perforin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

In vitro vascular differentiation system efficiently produces natural killer cells for cancer immunotherapies.

Oncoimmunology

Galat Y,Du Y,Perepitchka M,Li XN,Balyasnikova IV,Tse WT,Dambaeva S,Schneiderman S,Iannaccone PM,Becher O,Graham DK,Galat V

Published figure using Perforin monoclonal antibody (Product # 12-9994-42) in Flow Cytometry

Tue Sep 19 00:00:00 EDT 2023

CD8+ T cell-Dependent Remodeling of the Tumor Microenvironment Overcomes Chemoresistance.

Cancer immunology research

Lao L,Zeng W,Huang P,Chen H,Jia Z,Wang P,Huang D,Chen J,Nie Y,Yang L,Wu W,Liu J

Published figure using Perforin monoclonal antibody (Product # 12-9994-42) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

Rapamycin improves Graves' orbitopathy by suppressing CD4+ cytotoxic T lymphocytes.

JCI insight

Zhang M,Chong KK,Chen ZY,Guo H,Liu YF,Kang YY,Li YJ,Shi TT,Lai KK,He MQ,Ye K,Kahaly GJ,Shi BY,Wang Y

Published figure using Perforin monoclonal antibody (Product # 12-9994-42) in Flow Cytometry

Wed Feb 08 00:00:00 EST 2023

LINC01123 promotes immune escape by sponging miR-214-3p to regulate B7-H3 in head and neck squamous-cell carcinoma.

Cell death & disease

Li H,Yang Z,Yang X,Zhang F,Wang J,Wu Z,Wanyan C,Meng Q,Gao W,Yang X,Wei J

Published figure using Perforin monoclonal antibody (Product # 12-9994-42) in Flow Cytometry

Thu Feb 03 00:00:00 EST 2022

PD-1-Expressing SARS-CoV-2-Specific CD8⁺ T Cells Are Not Exhausted, but Functional in Patients with COVID-19.

Immunity

Rha MS,Jeong HW,Ko JH,Choi SJ,Seo IH,Lee JS,Sa M,Kim AR,Joo EJ,Ahn JY,Kim JH,Song KH,Kim ES,Oh DH,Ahn MY,Choi HK,Jeon JH,Choi JP,Kim HB,Kim YK,Park SH,Choi WS,Choi JY,Peck KR,Shin EC

Published figure using Perforin monoclonal antibody (Product # 12-9994-42) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Human Thymic CD10⁺ PD-1⁺ Intraepithelial Lymphocyte Precursors Acquire Interleukin-15 Responsiveness at the CD1a^- CD95⁺ CD28^- CCR7^- Developmental Stage.

International journal of molecular sciences

Billiet L,Goetgeluk G,Bonte S,De Munter S,De Cock L,Pille M,Ingels J,Jansen H,Weening K,Van Nieuwerburgh F,Kerre T,Taghon T,Leclercq G,Vandekerckhove B

12-9994 was used in Flow cytometry/Cell sorting to study the phenotypical and transcriptional differentiation of the thymic IEL precursor lineage upon in vitro exposure to cytokines prominent in the peripheral tissues such as interleukin-15 and the inflammatory cytokines interleukin-12 and interleukin-18.

Fri Nov 20 00:00:00 EST 2020

Human Thymic CD10⁺ PD-1⁺ Intraepithelial Lymphocyte Precursors Acquire Interleukin-15 Responsiveness at the CD1a^- CD95⁺ CD28^- CCR7^- Developmental Stage.

International journal of molecular sciences

Billiet L,Goetgeluk G,Bonte S,De Munter S,De Cock L,Pille M,Ingels J,Jansen H,Weening K,Van Nieuwerburgh F,Kerre T,Taghon T,Leclercq G,Vandekerckhove B

Fri Nov 20 00:00:00 EST 2020

NK and T Cell Differentiation at the Maternal-Fetal Interface in Sows During Late Gestation.

Frontiers in immunology

Stas MR,Koch M,Stadler M,Sawyer S,Sassu EL,Mair KH,Saalmüller A,Gerner W,Ladinig A

12-9994-42 was used in Flow Cytometry to investigate the immune cell phenotype on late gestation in pigs.

Tue Jun 22 00:00:00 EDT 2021

Interleukin-24 Regulates T Cell Activity in Patients With Colorectal Adenocarcinoma.

Frontiers in oncology

Zhang Y,Liu Y,Xu Y

12-9994-42 was used in Flow Cytometry to indicate that IL-24 might regulate T cell function in a dose-dependent manner.

Wed Sep 30 00:00:00 EDT 2020

A Bispecific Antibody to Link a TRAIL-Based Antitumor Approach to Immunotherapy.

Frontiers in immunology

Satta A,Grazia G,Caroli F,Frigerio B,Di Nicola M,Raspagliesi F,Mezzanzanica D,Zaffaroni N,Gianni AM,Anichini A,Figini M

Published figure using Perforin monoclonal antibody (Product # 12-9994-42) in Flow Cytometry

Mon Nov 02 00:00:00 EST 2020

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

12-9994 was used in Flow cytometry/Cell sorting to demonstrate that blocking PITPNM3 recognition of CCL18 could be an attractive immunotherapy aproach for treating breast cancer.

Sat Apr 01 00:00:00 EDT 2017

Zoledronic acid boosts γδ T-cell activity in children receiving αβ⁺ T and CD19⁺ cell-depleted grafts from an HLA-haplo-identical donor.

Oncoimmunology

Bertaina A,Zorzoli A,Petretto A,Barbarito G,Inglese E,Merli P,Lavarello C,Brescia LP,De Angelis B,Tripodi G,Moretta L,Locatelli F,Airoldi I

12-9994 was used in Flow cytometry/Cell sorting to indicate that ZOL infusion in pediatric recipients of αβ T- and B-cell-depleted HLA-haploidentical HSCT promotes γδ T-cell differentiation.

Fri Mar 11 00:00:00 EST 2022

Enhancing adoptive cancer immunotherapy with Vγ2Vδ2 T cells through pulse zoledronate stimulation.

Journal for immunotherapy of cancer

Nada MH,Wang H,Workalemahu G,Tanaka Y,Morita CT

12-9994 was used in Flow cytometry/Cell sorting to indicate pulse zoledronate stimulation maximises the purity, quantity and quality of expanded Vγ2Vδ2 cells for adoptive immunotherapy.

Wed Jun 06 00:00:00 EDT 2018

Added effects of dexamethasone and mesenchymal stem cells on early Natural Killer cell activation.

Transplant immunology

Michelo CM,Fasse E,van Cranenbroek B,Linda K,van der Meer A,Abdelrazik H,Joosten I

12-9994-42 was used in Flow cytometry/Cell sorting to examine the effects of Mesenchymal stem cells and selected immunosuppressive drugs (tacrolimus, cyclosporin A, mycophenolic acid, dexamethasone) treatment on early NK-cell activation.

Fri Jul 01 00:00:00 EDT 2016

Peritoneal carcinomatosis of colorectal cancer is characterized by structural and functional reorganization of the tumor microenvironment inducing senescence and proliferation arrest in cancer cells.

Oncoimmunology

Seebauer CT,Brunner S,Glockzin G,Piso P,Ruemmele P,Schlitt HJ,Geissler EK,Fichtner-Feigl S,Kesselring R

Published figure using Perforin monoclonal antibody (Product # 12-9994-42) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Reprogramming tumor-infiltrating dendritic cells for CD103+ CD8+ mucosal T-cell differentiation and breast cancer rejection.

Cancer immunology research

Wu TC,Xu K,Banchereau R,Marches F,Yu CI,Martinek J,Anguiano E,Pedroza-Gonzalez A,Snipes GJ,O'Shaughnessy J,Nishimura S,Liu YJ,Pascual V,Banchereau J,Oh S,Palucka K

12-9994 was used in Flow cytometry/Cell sorting to demonstrate that intratumoural delivery of the β-glucan curdlan blocks the generation of inflammatory Th2 cells, and prevents breast cancer progression in vivo.

Thu May 01 00:00:00 EDT 2014

Signal transducer and activator of transcription 3 (STAT3) mutations underlying autosomal dominant hyper-IgE syndrome impair human CD8(+) T-cell memory formation and function.

The Journal of allergy and clinical immunology

Ives ML,Ma CS,Palendira U,Chan A,Bustamante J,Boisson-Dupuis S,Arkwright PD,Engelhard D,Averbuch D,Magdorf K,Roesler J,Peake J,Wong M,Adelstein S,Choo S,Smart JM,French MA,Fulcher DA,Cook MC,Picard C,Durandy A,Tsumura M,Kobayashi M,Uzel G,Casanova JL,Tangye SG,Deenick EK

12-9994 was used in Flow cytometry/Cell sorting to investigate the role of IL-21 and associated pathways in CD8(+) T-cell immunity in humans, showing that STAT3 mutations impair CD8(+) T-cell memory formation and function.

Thu Aug 01 00:00:00 EDT 2013

DOCK8 deficiency impairs CD8 T cell survival and function in humans and mice.

The Journal of experimental medicine

Randall KL,Chan SS,Ma CS,Fung I,Mei Y,Yabas M,Tan A,Arkwright PD,Al Suwairi W,Lugo Reyes SO,Yamazaki-Nakashimada MA,Garcia-Cruz Mde L,Smart JM,Picard C,Okada S,Jouanguy E,Casanova JL,Lambe T,Cornall RJ,Russell S,Oliaro J,Tangye SG,Bertram EM,Goodnow CC

12-9994 was used in Flow cytometry/Cell sorting to study the role of DOCK8 in the survival and function of human and mouse CD8 T cells.

Mon Oct 24 00:00:00 EDT 2011

Virulence of Toxoplasma gondii is associated with distinct dendritic cell responses and reduced numbers of activated CD8+ T cells.

Journal of immunology (Baltimore, Md. : 1950)

Tait ED,Jordan KA,Dupont CD,Harris TH,Gregg B,Wilson EH,Pepper M,Dzierszinski F,Roos DS,Hunter CA

12-9994 was used in Flow cytometry/Cell sorting to show the dendritic cell response to T.gondii alters levels of CD8+ T cells which ultimately determine the outcome of infection.

Sun Aug 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: Cytolysin; lymphocyte pore forming protein; Lymphocyte pore-forming protein; OMAK; OTTHUMP00000019759; P1; perforin 1 (pore forming protein); Perforin-1; Perforin1; PFP; PGFL; PIGF; PIGF-2; PLGF; pore-forming protein; PRF1 (pore forming protein 1); RP11-710A11.3

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Gene Aliases: FLH2; HPLH2; P1; PFN1; PFP; PRF1

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UniProt ID: (Human) P14222

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Entrez Gene ID: (Human) 5551, (Pig) 396595

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Function(s)

calcium ion binding protein binding wide pore channel activity

Process(es)

immunological synapse formation defense response to tumor cell immune response to tumor cell apoptotic process cellular defense response cytolysis protein homooligomerization defense response to virus transmembrane transport

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Perforin Monoclonal Antibody (dG9 (delta G9)), PE, eBioscience™

Perforin Antibody (12-9994-42) in Flow

Product Details

12-9994-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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