Invitrogen

CD28 Monoclonal Antibody (37.51), Biotin, eBioscience™

FIGURE: 1 / 21

CD28 Antibody (13-0281-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.25 µg of Golden Syrian Hamster IgG Isotype Control Biotin (Product # 12-4914-81) (left) or 0.25 µg of Anti-Mouse CD28 Biotin (right) and then detected with Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

CD28 Antibody in Immunohistochemistry (IHC)

CD28 Antibody in Flow Cytometry, T-Cell Activation (Flow, TCA)

CD28 Antibody in T-Cell Activation (TCA)

Product Image Gallery

Product Details

13-0281-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 11 publications 11 publications

T-Cell Activation (TCA)

View 8 publications 8 publications

Affinity Purification (AP)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Syrian hamster / IgG

Recommended Isotype Control

Syrian Hamster IgG Isotype Control, Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

37.51

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466411

Product Specific Information

Description: The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45 kDa homodimer expressed by thymocytes, mature T cells and NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.

Applications Reported: The 37.51 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 37.51 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Note: It has been observed that increased incubations times (30-45 minutes) with the CD28 antibody can enhance the staining obtained.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Senescent T Cell Induces Brown Adipose Tissue "Whitening" Via Secreting IFN-γ.

Frontiers in cell and developmental biology

Pan XX,Yao KL,Yang YF,Ge Q,Zhang R,Gao PJ,Ruan CC,Wu F

Published figure using CD28 monoclonal antibody (Product # 13-0281-82) in Flow Cytometry

Tue Mar 23 00:00:00 EDT 2021

VX-765 reduces neuroinflammation after spinal cord injury in mice.

Neural regeneration research

Chen J,Chen YQ,Shi YJ,Ding SQ,Shen L,Wang R,Wang QY,Zha C,Ding H,Hu JG,Lü HZ

Published figure using CD28 monoclonal antibody (Product # 13-0281-82) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 2021

Activated autophagy restored the impaired frequency and function of regulatory T cells in chronic prostatitis.

The Prostate

Liu Y,Zhang Y,Zhang M,Meng J,Ma Q,Hao Z,Zheng M,Zhang L,Chen X,Liang C

Published figure using CD28 monoclonal antibody (Product # 13-0281-82) in T-Cell Activation

Fri Jan 01 00:00:00 EST 2021

Cytotoxic T cells swarm by homotypic chemokine signalling.

eLife

Galeano Niño JL,Pageon SV,Tay SS,Colakoglu F,Kempe D,Hywood J,Mazalo JK,Cremasco J,Govendir MA,Dagley LF,Hsu K,Rizzetto S,Zieba J,Rice G,Prior V,O'Neill GM,Williams RJ,Nisbet DR,Kramer B,Webb AI,Luciani F,Read MN,Biro M

13-0281 was used in Flow cytometry/Cell sorting to reveal independent emergent behaviour in Cytotoxic T lymphocytes populations attacking tumour masses.

Tue Oct 13 00:00:00 EDT 2020

C-Reactive Protein Promotes the Expansion of Myeloid Derived Cells With Suppressor Functions.

Frontiers in immunology

Jimenez RV,Kuznetsova V,Connelly AN,Hel Z,Szalai AJ

Published figure using CD28 monoclonal antibody (Product # 13-0281-82) in T-Cell Activation

Tue Nov 10 00:00:00 EST 2020

Tpl2 Protects Against Fulminant Hepatitis Through Mobilization of Myeloid-Derived Suppressor Cells.

Frontiers in immunology

Xu J,Pei S,Wang Y,Liu J,Qian Y,Huang M,Zhang Y,Xiao Y

Published figure using CD28 monoclonal antibody (Product # 13-0281-82) in T-Cell Activation

Wed Oct 14 00:00:00 EDT 2020

Virus-Induced Interferon-γ Causes Insulin Resistance in Skeletal Muscle and Derails Glycemic Control in Obesity.

Immunity

Šestan M,Marinović S,Kavazović I,Cekinović Đ,Wueest S,Turk Wensveen T,Brizić I,Jonjić S,Konrad D,Wensveen FM,Polić B

13-0281 was used in Flow cytometry/Cell sorting to study how viral infection effects insulin resistance.

Tue Jul 17 00:00:00 EDT 2018

Immunization with α-synuclein/Grp94 reshapes peripheral immunity and suppresses microgliosis in a chronic Parkinsonism model.

Glia

Villadiego J,Labrador-Garrido A,Franco JM,Leal-Lasarte M,De Genst EJ,Dobson CM,Pozo D,Toledo-Aral JJ,Roodveldt C

Published figure using CD28 monoclonal antibody (Product # 13-0281-82) in T-Cell Activation

Mon Jan 01 00:00:00 EST 2018

Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.

eLife

Moretti FA,Klapproth S,Ruppert R,Margraf A,Weber J,Pick R,Scheiermann C,Sperandio M,Fässler R,Moser M

Published figure using CD28 monoclonal antibody (Product # 13-0281-82) in Flow Cytometry

Thu Sep 06 00:00:00 EDT 2018

T cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells.

Nature communications

Kim HR,Mun Y,Lee KS,Park YJ,Park JS,Park JH,Jeon BN,Kim CH,Jun Y,Hyun YM,Kim M,Lee SM,Park CS,Im SH,Jun CD

Published figure using CD28 monoclonal antibody (Product # 13-0281-82) in Western Blot

Fri Sep 07 00:00:00 EDT 2018

CTCF-Mediated Enhancer-Promoter Interaction Is a Critical Regulator of Cell-to-Cell Variation of Gene Expression.

Molecular cell

Ren G,Jin W,Cui K,Rodrigez J,Hu G,Zhang Z,Larson DR,Zhao K

Published figure using CD28 monoclonal antibody (Product # 13-0281-82) in Flow Cytometry

Thu Sep 21 00:00:00 EDT 2017

The ER membrane-anchored ubiquitin ligase Hrd1 is a positive regulator of T-cell immunity.

Nature communications

Xu Y,Zhao F,Qiu Q,Chen K,Wei J,Kong Q,Gao B,Melo-Cardenas J,Zhang B,Zhang J,Song J,Zhang DD,Zhang J,Fan Y,Li H,Fang D

Published figure using CD28 monoclonal antibody (Product # 13-0281-82) in Flow Cytometry

Fri Jul 15 00:00:00 EDT 2016

The ER membrane-anchored ubiquitin ligase Hrd1 is a positive regulator of T-cell immunity.

Nature communications

Xu Y,Zhao F,Qiu Q,Chen K,Wei J,Kong Q,Gao B,Melo-Cardenas J,Zhang B,Zhang J,Song J,Zhang DD,Zhang J,Fan Y,Li H,Fang D

Published figure using CD28 monoclonal antibody (Product # 13-0281-82) in Flow Cytometry

Fri Jul 15 00:00:00 EDT 2016

Apurinic/Apyrimidinic Endonuclease 1/Redox Factor-1 (Ape1/Ref-1) Modulates Antigen Presenting Cell-mediated T Helper Cell Type 1 Responses.

The Journal of biological chemistry

Akhter N,Takeda Y,Nara H,Araki A,Ishii N,Asao N,Asao H

Published figure using CD28 monoclonal antibody (Product # 13-0281-82) in T-Cell Activation

Fri Nov 04 00:00:00 EDT 2016

Melatonin Contributes to the Seasonality of Multiple Sclerosis Relapses.

Cell

Farez MF,Mascanfroni ID,Méndez-Huergo SP,Yeste A,Murugaiyan G,Garo LP,Balbuena Aguirre ME,Patel B,Ysrraelit MC,Zhu C,Kuchroo VK,Rabinovich GA,Quintana FJ,Correale J

Published figure using CD28 monoclonal antibody (Product # 13-0281-82) in T-Cell Activation

Thu Sep 10 00:00:00 EDT 2015

Interleukin-7 and anti-programmed cell death 1 antibody have differing effects to reverse sepsis-induced immunosuppression.

Shock (Augusta, Ga.)

Shindo Y,Unsinger J,Burnham CA,Green JM,Hotchkiss RS

Published figure using CD28 monoclonal antibody (Product # 13-0281-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Overexpression of IL-15 promotes tumor destruction via NK1.1+ cells in a spontaneous breast cancer model.

BMC cancer

Gillgrass AE,Chew MV,Krneta T,Ashkar AA

Published figure using CD28 monoclonal antibody (Product # 13-0281-82) in Immunohistochemistry

Thu Apr 16 00:00:00 EDT 2015

Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus.

Scientific reports

Christo SN,Diener KR,Nordon RE,Brown MP,Griesser HJ,Vasilev K,Christo FC,Hayball JD

13-0281 was used in Affinity chromatography to demonstrate a new method to evaluate graduations in T cell activation in real-time and on an individual cell basis.

Wed Jan 14 00:00:00 EST 2015

B7-CD28 costimulatory signals control the survival and proliferation of murine and human γδ T cells via IL-2 production.

Journal of immunology (Baltimore, Md. : 1950)

Ribot JC,Debarros A,Mancio-Silva L,Pamplona A,Silva-Santos B

13-0281 was used in Flow cytometry/Cell sorting to show that CD28 signals are required for IL-2-mediated survival and proliferation of both CD27(+) and CD27(-) γδ T cell subsets.

Wed Aug 01 00:00:00 EDT 2012

Galectin-9 controls CD40 signaling through a Tim-3 independent mechanism and redirects the cytokine profile of pathogenic T cells in autoimmunity.

PloS one

Vaitaitis GM,Wagner DH

13-0281 was used in Magnetic cell separation to study the mechanisms underlying control of the CD40 receptor and its role in the etiology of autoimmunity.

Fri Dec 07 00:00:00 EST 2012

Lymphotoxin beta receptor signaling promotes tertiary lymphoid organogenesis in the aorta adventitia of aged ApoE-/- mice.

The Journal of experimental medicine

Gräbner R,Lötzer K,Döpping S,Hildner M,Radke D,Beer M,Spanbroek R,Lippert B,Reardon CA,Getz GS,Fu YX,Hehlgans T,Mebius RE,van der Wall M,Kruspe D,Englert C,Lovas A,Hu D,Randolph GJ,Weih F,Habenicht AJ

13-0281 was used in Flow cytometry/Cell sorting to investigate how the LTbetaR pathway has a major role in shaping the immunological characteristics and overall integrity of the arterial wall.

Fri Jan 16 00:00:00 EST 2009

NKG2A inhibits invariant NKT cell activation in hepatic injury.

Journal of immunology (Baltimore, Md. : 1950)

Kawamura T,Takeda K,Kaneda H,Matsumoto H,Hayakawa Y,Raulet DH,Ikarashi Y,Kronenberg M,Yagita H,Kinoshita K,Abo T,Okumura K,Smyth MJ

13-0281 was used in Flow cytometry/Cell sorting to investigate the role of NK cell receptors in iNKT cell activation, showing that NKG2A-mediated signalling negatively regulated iNKT activation and hepatic injury.

Thu Jan 01 00:00:00 EST 2009

Phosphorylation of Bcl10 negatively regulates T-cell receptor-mediated NF-kappaB activation.

Molecular and cellular biology

Zeng H,Di L,Fu G,Chen Y,Gao X,Xu L,Lin X,Wen R

Published figure using CD28 monoclonal antibody (Product # 13-0281-82) in T-Cell Activation

Sun Jul 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: CD28; MGC138290; sCD28; soluble CD28; T-cell-specific surface glycoprotein CD28; T44

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Gene Aliases: Cd28

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UniProt ID: (Mouse) P31041

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Entrez Gene ID: (Mouse) 12487

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Function(s)

protease binding SH3/SH2 adaptor activity protein homodimerization activity immunoglobulin receptor superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD28 Monoclonal Antibody (37.51), Biotin, eBioscience™

CD28 Antibody (13-0281-82) in Flow

Product Details

13-0281-82

Western Blot (WB)

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

T-Cell Activation (TCA)

Affinity Purification (AP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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