Invitrogen

CD51 (Integrin alpha V) Monoclonal Antibody (RMV-7), Biotin, eBioscience™

8 Published Figures

16 References

View all (31) Integrin alpha V (CD51) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (8)

CD51 (Integrin alpha V) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 9

CD51 (Integrin alpha V) Antibody (13-0512-82) in Flow

Staining of C57BL/6 bone marrow cells with 0.5 µg of Rat IgG1 kappa Isotype Control Biotin (Product # 13-4301-82) (open histogram) or 0.5 µg of Anti-Mouse CD51 (Integrin alpha V) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Cells in the large scatter population were used for analysis.

CD51 (Integrin alpha V) Antibody in Immunohistochemistry (IHC)

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Product Details

13-0512-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Flow Cytometry (Flow)

1 µg/test

View 13 publications 13 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RMV-7

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466477

Product Specific Information

Description: The RMV-7 monoclonal antibody reacts with the mouse CD51 molecule, the integrin alpha v chain. This approximately 120 kDa surface molecule non-covalently associates with the beta subunits of the integrin family including beta3 (CD61), beta1 (CD29), beta5 and beta6 to form receptors for extracellular matrix components. Heterodimers of CD51/CD61 are expressed by platelets, T cells and granulocytes and mediate adhesion to fibrinogen, fibronectin, vitronectin and thrombospondin.

Applications Reported: The RMV-7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The RMV-7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8, 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands, the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Isolation and in vitro characterization of murine young-adult long bone skeletal progenitors.

Frontiers in endocrinology

Loopmans S,Stockmans I,Carmeliet G,Stegen S

13-0512-82 was used in Flow cytometry/Cell sorting to propose a validated isolation and culture protocol to study metaphyseal/endosteal SSPC biology in vitro.

Fri Aug 19 00:00:00 EDT 2022

Heterogeneity of murine periosteum progenitors involved in fracture healing.

eLife

Matthews BG,Novak S,Sbrana FV,Funnell JL,Cao Y,Buckels EJ,Grcevic D,Kalajzic I

13-0512-82 was used in Flow Cytometry to demonstrate that the periosteum is highly enriched with skeletal progenitor cells, and there is heterogeneity in the populations of cells that contribute to mature lineages during periosteal fracture healing.

Tue Feb 09 00:00:00 EST 2021

TNF-α-induced alterations in stromal progenitors enhance leukemic stem cell growth via CXCR2 signaling.

Cell reports

Agarwal P,Li H,Choi K,Hueneman K,He J,Welner RS,Starczynowski DT,Bhatia R

13-0512-82 was used in Flow Cytometry to find that TNF-α-mediated alterations in CML BM stromal niches enhance support of LSC maintenance and growth via CXCL1-CXCR2 signaling and that CXCR2 inhibition effectively depletes CML LSCs.

Tue Jul 13 00:00:00 EDT 2021

A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation.

Nature communications

Kim JM,Yang YS,Park KH,Ge X,Xu R,Li N,Song M,Chun H,Bok S,Charles JF,Filhol-Cochet O,Boldyreff B,Dinter T,Yu PB,Kon N,Gu W,Takarada T,Greenblatt MB,Shim JH

Published figure using CD51 (Integrin alpha V) monoclonal antibody (Product # 13-0512-82) in Flow Cytometry

Fri May 08 00:00:00 EDT 2020

Nestin-GFP transgene labels skeletal progenitors in the periosteum.

Bone

Tournaire G,Stegen S,Giacomini G,Stockmans I,Moermans K,Carmeliet G,van Gastel N

13-0512-82 was used in Flow Cytometry to show that in adult mice, Nes-GFP cells are present in the periosteum and localize closely to blood vessels, but periosteal Nes-GFP cells express SSC and progenitor markers differently compared to Nes-GFP cells in the bone marrow.

Wed Apr 01 00:00:00 EDT 2020

Engineering a haematopoietic stem cell niche by revitalizing mesenchymal stromal cells.

Nature cell biology

Nakahara F,Borger DK,Wei Q,Pinho S,Maryanovich M,Zahalka AH,Suzuki M,Cruz CD,Wang Z,Xu C,Boulais PE,Ma'ayan A,Greally JM,Frenette PS

13-0512-82 was used in Flow Cytometry to provide insight into the transcriptional regulation of the niche with implications for stem cell-based therapies.

Wed May 01 00:00:00 EDT 2019

Mesenchymal Niche-Specific Expression of Cxcl12 Controls Quiescence of Treatment-Resistant Leukemia Stem Cells.

Cell stem cell

Agarwal P,Isringhausen S,Li H,Paterson AJ,He J,Gomariz Á,Nagasawa T,Nombela-Arrieta C,Bhatia R

13-0512-82 was used in Flow Cytometry to highlight a critical role of niche-specific effects of Cxcl12 expression in maintaining quiescence of TKI-resistant LSC populations.

Thu May 02 00:00:00 EDT 2019

Soluble Thy-1 reverses lung fibrosis via its integrin-binding motif.

JCI insight

Tan C,Jiang M,Wong SS,Espinoza CR,Kim C,Li X,Connors E,Hagood JS

Published figure using CD51 (Integrin alpha V) monoclonal antibody (Product # 13-0512-82) in Immunohistochemistry

Fri Nov 01 00:00:00 EDT 2019

Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche.

Cell reports

Balzano M,De Grandis M,Vu Manh TP,Chasson L,Bardin F,Farina A,Sergé A,Bidaut G,Charbord P,Hérault L,Bailly AL,Cartier-Michaud A,Boned A,Dalod M,Duprez E,Genever P,Coles M,Bajenoff M,Xerri L,Aurrand-Lions M,Schiff C,Mancini SJC

13-0512 was used in Flow cytometry/Cell sorting to study pro-B cells in their support of stroll cells and their interaction network allowing their retention.

Tue Mar 19 00:00:00 EDT 2019

Adrenergic nerve degeneration in bone marrow drives aging of the hematopoietic stem cell niche.

Nature medicine

Maryanovich M,Zahalka AH,Pierce H,Pinho S,Nakahara F,Asada N,Wei Q,Wang X,Ciero P,Xu J,Leftin A,Frenette PS

Published figure using CD51 (Integrin alpha V) monoclonal antibody (Product # 13-0512-82) in Flow Cytometry

Fri Jun 01 00:00:00 EDT 2018

Age-specific biological and molecular profiling distinguishes paediatric from adult acute myeloid leukaemias.

Nature communications

Chaudhury S,O'Connor C,Cañete A,Bittencourt-Silvestre J,Sarrou E,Prendergast Á,Choi J,Johnston P,Wells CA,Gibson B,Keeshan K

Published figure using CD51 (Integrin alpha V) monoclonal antibody (Product # 13-0512-82) in Flow Cytometry

Tue Dec 11 00:00:00 EST 2018

Systemic delivery of a breast cancer-detecting adenovirus using targeted microbubbles.

Cancer gene therapy

Warram JM,Sorace AG,Saini R,Borovjagin AV,Hoyt K,Zinn KR

13-0512 was used in Flow cytometry/Cell sorting to investigate methods to prevent inactivation of recombinant human adenovirus for the treatment of cancer gene therapy, showing the potential for systemic delivery of a breast cancer-detecting adenovirus using targeted microbubbles.

Wed Aug 01 00:00:00 EDT 2012

Molecular ultrasound imaging using a targeted contrast agent for assessing early tumor response to antiangiogenic therapy.

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine

Sorace AG,Saini R,Mahoney M,Hoyt K

13-0512 was used in in vivo experiments to explore a novel method for angiogenesis evaluation and disease staging in cancer.

Mon Oct 01 00:00:00 EDT 2012

Molecular ultrasound imaging using a targeted contrast agent for assessing early tumor response to antiangiogenic therapy.

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine

Sorace AG,Saini R,Mahoney M,Hoyt K

13-0512 was used in in vivo experiments to explore a novel method for angiogenesis evaluation and disease staging in cancer.

Mon Oct 01 00:00:00 EDT 2012

A triple-targeted ultrasound contrast agent provides improved localization to tumor vasculature.

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine

Warram JM,Sorace AG,Saini R,Umphrey HR,Zinn KR,Hoyt K

13-0512 was used in Conjugation experiments to evaluate multitargeted microbubbles used for tumoural sonography, comparing single-, dual-, and triple-targeted motifs.

Fri Jul 01 00:00:00 EDT 2011

A triple-targeted ultrasound contrast agent provides improved localization to tumor vasculature.

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine

Warram JM,Sorace AG,Saini R,Umphrey HR,Zinn KR,Hoyt K

13-0512 was used in Conjugation experiments to evaluate multitargeted microbubbles used for tumoural sonography, comparing single-, dual-, and triple-targeted motifs.

Fri Jul 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: alpha v integrin; CD51; GP3A; Integrin alpha-V; ITGB3; vitronectin receptor alpha polypeptide (VNRA); Vitronectin receptor subunit alpha; VNR

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Gene Aliases: 1110004F14Rik; 2610028E01Rik; CD51; D430040G12Rik; Itgav

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UniProt ID: (Mouse) P43406

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Entrez Gene ID: (Mouse) 16410

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Function(s)

opsonin binding fibronectin binding protease binding protein kinase C binding receptor binding voltage-gated calcium channel activity insulin-like growth factor I binding peptide binding metal ion binding protein heterodimerization activity extracellular matrix binding extracellular matrix protein binding integrin

Process(es)

angiogenesis blood vessel development cellular calcium ion homeostasis inflammatory response cell adhesion cell-matrix adhesion positive regulation of cytosolic calcium ion concentration integrin-mediated signaling pathway multicellular organism development positive regulation of cell proliferation fertilization negative regulation of macrophage derived foam cell differentiation negative regulation of lipid storage cell growth cell migration cell differentiation positive regulation of cell migration cell-substrate adhesion negative regulation of lipid transport detection of molecule of bacterial origin positive regulation of osteoblast proliferation heterotypic cell-cell adhesion substrate adhesion-dependent cell spreading regulation of ion transmembrane transport gonad morphogenesis endodermal cell differentiation apolipoprotein A-I-mediated signaling pathway T cell activation apoptotic cell clearance positive regulation of MAPK cascade endothelial cell migration regulation of bone resorption negative regulation of low-density lipoprotein particle receptor biosynthetic process positive regulation of cell adhesion viral entry into host cell focal adhesion assembly negative regulation of lipoprotein metabolic process regulation of phagocytosis negative chemotaxis release of sequestered calcium ion into cytosol trophoblast giant cell differentiation ERK1 and ERK2 cascade calcium ion transmembrane transport response to nitric oxide extrinsic apoptotic signaling pathway in absence of ligand regulation of apoptotic cell clearance negative regulation of entry of bacterium into host cell negative regulation of extrinsic apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD51 (Integrin alpha V) Monoclonal Antibody (RMV-7), Biotin, eBioscience™

CD51 (Integrin alpha V) Antibody (13-0512-82) in Flow

Product Details

13-0512-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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