Invitrogen

NEFH Monoclonal Antibody (RMdO-20)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 Published Figures

14 References

View all (65) NF-H antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

NEFH Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 15

NEFH Antibody (13-1300) in IHC (P)

Immunohistochemistry analysis of the neurofilament heavy chain showing staining in the filaments of paraffin-embedded mouse brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 m... View More

NEFH Antibody in Immunohistochemistry (Frozen) (IHC (F))

NEFH Antibody in Immunocytochemistry (ICC/IF)

NEFH Antibody in Immunohistochemistry (IHC)

Product Image Gallery

NEFH Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of NEFH was observed in SH-SY5Y and IMR-32 in comparison to LNCaP and MCF7 using Anti-NEFH Monoclonal Antibody (RMdO-20) (Product # 13-1300) in Western Blot. {RE}

Product Details

13-1300

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

1:20

Immunocytochemistry (ICC/IF)

1:20

View 5 publications 5 publications

ELISA (ELISA)

0.1-0.5 µg/mL

Immunoprecipitation (IP)

2-5 µg

Product Specifications

Species Reactivity

Bovine, Hamster, Human, Mollusc, Mouse, Rabbit, Rat

Published species

Human, Mouse, Rabbit, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

RMdO-20

Immunogen

Adult Rat neurofilaments

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2532999

Product Specific Information

This antibody reacts with 200 kDa subunit protein of human neurofilament. It specifically recognizes a nonphosphorylated epitope in the tail domain of NF-H. Dephosphorylation of NF-H will increase immunoreactivity with this antibody.

This antibody is suitable for immunohistochemical staining of Bouin's and alcohol-fixed paraffin-embedded or frozen tissue sections. To stain, incubate 30-60 minutes at room temperature or overnight at 4°C.

Target Information

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-H has an important role in mature axons and is involved in amyotrophic lateral sclerosis (ALS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Platelet-rich plasma promotes peripheral nerve regeneration after sciatic nerve injury.

Neural regeneration research

Wang SL,Liu XL,Kang ZC,Wang YS

13-1300 was used in Immunohistochemistry to suggest that platelet-rich plasma promotes peripheral nerve regeneration after sciatic nerve injury by affecting angiogenesis and intracellular ubiquitin levels.

Wed Feb 01 00:00:00 EST 2023

Platelet-rich plasma promotes peripheral nerve regeneration after sciatic nerve injury.

Neural regeneration research

Wang SL,Liu XL,Kang ZC,Wang YS

Wed Feb 01 00:00:00 EST 2023

Unique Neural Circuit Connectivity of Mouse Proximal, Middle, and Distal Colon Defines Regional Colonic Motor Patterns.

Cellular and molecular gastroenterology and hepatology

Nestor-Kalinoski A,Smith-Edwards KM,Meerschaert K,Margiotta JF,Rajwa B,Davis BM,Howard MJ

13-1300 was used in Immunohistochemistry to describe quantitatively, by region, the structural architecture of the mouse enteric nervous system and show regional underpinnings of different motor patterns.

Mon Apr 04 00:00:00 EDT 2022

Application of immune enhanced organoids in modeling personalized Merkel cell carcinoma research.

Scientific reports

Forsythe SD,Erali RA,Laney P,Sivakumar H,Li W,Skardal A,Soker S,Votanopoulos KI

13-1300 was used in Immunohistochemistry to study the application of patient tumor organoids (PTOs) in modelling personalized research in Merkel cell carcinoma (MCC).

Tue Aug 16 00:00:00 EDT 2022

Gene therapy via canalostomy approach preserves auditory and vestibular functions in a mouse model of Jervell and Lange-Nielsen syndrome type 2.

Nature communications

Wu X,Zhang L,Li Y,Zhang W,Wang J,Cai C,Lin X

13-1300 was used in Immunocytochemistry to conduct gene therapy by injecting viral vectors using the canalostomy approach in Kcne1-/- mice to treat both the hearing and vestibular symptoms.

Fri Jan 29 00:00:00 EST 2021

3D Culture Method for Alzheimer's Disease Modeling Reveals Interleukin-4 Rescues Aβ42-Induced Loss of Human Neural Stem Cell Plasticity.

Developmental cell

Papadimitriou C,Celikkaya H,Cosacak MI,Mashkaryan V,Bray L,Bhattarai P,Brandt K,Hollak H,Chen X,He S,Antos CL,Lin W,Thomas AK,Dahl A,Kurth T,Friedrichs J,Zhang Y,Freudenberg U,Werner C,Kizil C

13-1300 was used in Immunocytochemistry-immunoflourescence to conclude that our culture system enables a reductionist investigation of regulation of human NSC plasticity for the identification of potential therapeutic targets for intervention in AD.

Mon Jul 02 00:00:00 EDT 2018

Sustained release of neurotrophin-3 via calcium phosphate-coated sutures promotes axonal regeneration after spinal cord injury.

Journal of neuroscience research

Hanna A,Thompson DL,Hellenbrand DJ,Lee JS,Madura CJ,Wesley MG,Dillon NJ,Sharma T,Enright CJ,Murphy WL

13-1300 was used in immunohistochemistry - paraffin section to learn promotion of axonal regeneration after spinal cord injury due to sustained release of neurotrophin-3 via calcium phosphate-coated sutures

Fri Jul 01 00:00:00 EDT 2016

Cerebral and spinal cord tanycytic ependymomas in a young adult with a mutation in the NF2 gene.

Neuropathology : official journal of the Japanese Society of Neuropathology

Kuga Y,Ohnishi H,Kodama Y,Takakura S,Hayashi M,Yagi R,Fukutome K,Matsushima K,Okamoto K,Taomoto K,Takahashi H

13-1300 was used in immunohistochemistry - paraffin section to determine the immunohistochemical, ultrastructural and genetic features of a patient with tanycytic ependymoma and that has a heterozygous truncating mutation in the NF2 gene

Fri Aug 01 00:00:00 EDT 2014

Evolution of neuronal and endothelial transcriptomes in primates.

Genome biology and evolution

Giger T,Khaitovich P,Somel M,Lorenc A,Lizano E,Harris LW,Ryan MM,Lan M,Wayland MT,Bahn S,Pääbo S

13-1300 was used in Immunohistochemistry to suggest that neuronal and endothelial transcriptomes evolve at different rates within brain tissue.

Mon Jul 12 00:00:00 EDT 2010

Glutamate receptor expression and chronic glutamate toxicity in rat motor cortex.

Neurobiology of disease

Young KC,McGehee DS,Brorson JR

13-1300 was used in immunohistochemistry to study the glutamate receptors on corticospinal neurons in acutely isolated rat motor cortex slices

Sun Apr 01 00:00:00 EDT 2007

Conversion of myoblasts to physiologically active neuronal phenotype.

Genes & development

Watanabe Y,Kameoka S,Gopalakrishnan V,Aldape KD,Pan ZZ,Lang FF,Majumder S

Thu Apr 15 00:00:00 EDT 2004

Conversion of myoblasts to physiologically active neuronal phenotype.

Genes & development

Watanabe Y,Kameoka S,Gopalakrishnan V,Aldape KD,Pan ZZ,Lang FF,Majumder S

Thu Apr 15 00:00:00 EDT 2004

Double immunolabeling of central nervous system atypical teratoid/rhabdoid tumors.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Bouffard JP,Sandberg GD,Golden JA,Rorke LB

13-1300 was used in immunohistochemistry to characterize central nervous system atypical teratoid/rhabdoid tumors

Tue Jun 01 00:00:00 EDT 2004

Role of the sensory neuron cytoskeleton in second messenger signaling for inflammatory pain.

Neuron

Dina OA,McCarter GC,de Coupade C,Levine JD

Thu Aug 14 00:00:00 EDT 2003

Mouse embryos cloned from brain tumors.

Cancer research

Li L,Connelly MC,Wetmore C,Curran T,Morgan JI

Sun Jun 01 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: 200 kDa neurofilament protein; NEF3; neurofilament; neurofilament 200kDa; Neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide; neurofilament, heavy polypeptide 200kDa; neurofilament-H; NF 200 kD; NF-H; NHC

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Gene Aliases: CMT2CC; I79_012512; KIAA0845; mKIAA0845; NEFH; NF-H; NF200; NFH

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UniProt ID: (Human) P12036, (Mouse) P19246

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Entrez Gene ID: (Human) 4744, (Rabbit) 100357177, (Chinese hamster) 100753127, (Rat) 24587, (Mouse) 380684

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Function(s)

structural molecule activity structural constituent of cytoskeleton microtubule binding toxic substance binding kinesin binding protein kinase binding protein binding, bridging dynein binding protein heterodimerization activity nutrient reservoir activity

Process(es)

microtubule cytoskeleton organization ovarian follicle atresia axonogenesis spinal cord development hippocampus development cerebral cortex development cell projection assembly axon regeneration neurofilament bundle assembly cellular response to oxidative stress response to cocaine peripheral nervous system neuron axonogenesis axon development cellular response to estradiol stimulus regulation of organelle transport along microtubule response to sodium arsenite response to acrylamide brain development embryo development neuron projection development intermediate filament cytoskeleton organization intermediate filament bundle assembly neurofilament cytoskeleton organization

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NEFH Monoclonal Antibody (RMdO-20)

NEFH Antibody (13-1300) in IHC (P)

Product Details

13-1300

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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