Invitrogen

CD4 Monoclonal Antibody (N1UG0), Biotin, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

6 Published Figures

7 References

View all (606) CD4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 9

CD4 Antibody (13-2444-82) in IHC (P)

Immunohistochemistry of formalin-fixed paraffin embedded human tonsil using 10 µg/mL of Anti-Human CD4 Biotin, followed by Streptavidin HRP, and DAB visualization. Nuclei are counterstained with hematoxylin.

CD4 Antibody in Immunohistochemistry (IHC)

CD4 Antibody in Immunocytochemistry (ICC/IF)

CD4 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Product Image Gallery

CD4 Antibody — Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD3 FITC, CD8 PE and CD4 Biotin followed by staining with APC conjugated streptavidin. As expected based on known relative expression patterns, CD4 clone N1UG0 stains a subset of lymphocytes (pink) and monocytes (orange), but not granulocytes (blue). {RE}

Product Details

13-2444-82

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

0.25 µg/test

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

N1UG0

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572780

Product Specific Information

Description: The N1UG0 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The N1UG0 antibody is recommended for use in staining human formalin-fixed paraffin embedded tissue sections.

Applications Reported: This N1UG0 antibody has been reported for use in flow cytometric analysis, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This N1UG0 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low or high pH antigen retrieval and can be used at less than or equal to 10 µg/mL. The N1UG0 antibody has been tested by flow cytometric analysis of human PBMC. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Intrahepatic activated leukocyte cell adhesion molecule induces CD6^highCD4⁺ T cell infiltration in autoimmune hepatitis.

Frontiers in immunology

Qian Q,Cui N,Huang B,Zhao Y,Liu Q,Hu M,Li B,Wang Q,Miao Q,You Z,Ma X,Tang R

Published figure using CD4 monoclonal antibody (Product # 13-2444-82) in Immunohistochemistry

Wed Sep 28 00:00:00 EDT 2022

Asymmetric T cell division of GAD65 specific naive T cells contribute to an early divergence in the differentiation fate into memory T cell subsets.

Immunology

Alampi G,Vignali D,Centorame I,Canu A,Cosorich I,Filoni J,Di Dedda C,Monti P

Published figure using CD4 monoclonal antibody (Product # 13-2444-82) in Immunocytochemistry

Tue Nov 01 00:00:00 EDT 2022

Molecular Mechanism of Sphingosine-1-Phosphate Receptor 1 Regulating CD4⁺ Tissue Memory in situ T Cells in Primary Sjogren's Syndrome.

International journal of general medicine

Yang XX,Yang C,Wang L,Zhou YB,Yuan X,Xiang N,Wang YP,Li XM

Published figure using CD4 monoclonal antibody (Product # 13-2444-82) in Immunocytochemistry

Wed Apr 27 00:00:00 EDT 2022

Molecular Mechanism of Sphingosine-1-Phosphate Receptor 1 Regulating CD4⁺ Tissue Memory in situ T Cells in Primary Sjogren's Syndrome.

International journal of general medicine

Yang XX,Yang C,Wang L,Zhou YB,Yuan X,Xiang N,Wang YP,Li XM

Published figure using CD4 monoclonal antibody (Product # 13-2444-82) in Immunocytochemistry

Wed Apr 27 00:00:00 EDT 2022

CD161 contributes to prenatal immune suppression of IFNγ-producing PLZF+ T cells.

The Journal of clinical investigation

Halkias J,Rackaityte E,Hillman SL,Aran D,Mendoza VF,Marshall LR,MacKenzie TC,Burt TD

Published figure using CD4 monoclonal antibody (Product # 13-2444-82) in Immunohistochemistry

Thu May 30 00:00:00 EDT 2019

Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes.

eLife

Lin JR,Izar B,Wang S,Yapp C,Mei S,Shah PM,Santagata S,Sorger PK

Published figure using CD4 monoclonal antibody (Product # 13-2444-82) in Immunofluorescence

Wed Jul 11 00:00:00 EDT 2018

Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes.

eLife

Lin JR,Izar B,Wang S,Yapp C,Mei S,Shah PM,Santagata S,Sorger PK

Published figure using CD4 monoclonal antibody (Product # 13-2444-82) in Immunofluorescence

Wed Jul 11 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; cd4a; fCD4; Leu-3; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

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Gene Aliases: CD4; CD4mut

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UniProt ID: (Human) P01730

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Entrez Gene ID: (Human) 920

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Function(s)

virus receptor activity glycoprotein binding receptor activity transmembrane signaling receptor activity extracellular matrix structural constituent protein binding zinc ion binding coreceptor activity immunoglobulin binding enzyme binding protein kinase binding MHC class II protein binding protein homodimerization activity protein tyrosine kinase binding

Process(es)

cytokine production adaptive immune response induction by virus of host cell-cell fusion immune response signal transduction cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of calcium ion transport into cytosol fusion of virus membrane with host plasma membrane T cell differentiation entry into host cell T cell costimulation response to estradiol maintenance of protein location in cell response to vitamin D helper T cell enhancement of adaptive immune response positive regulation of T cell proliferation T cell selection positive regulation of interleukin-2 biosynthetic process positive regulation of protein kinase activity regulation of defense response to virus by virus positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling T cell receptor signaling pathway regulation of T cell activation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD4 Monoclonal Antibody (N1UG0), Biotin, eBioscience™

CD4 Antibody (13-2444-82) in IHC (P)

Product Details

13-2444-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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