Invitrogen

CD178 (Fas Ligand) Monoclonal Antibody (MFL3), Biotin, eBioscience™

7 Published Figures

13 References

View all (46) Fas Ligand (CD178) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (7)

CD178 (Fas Ligand) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 8

CD178 (Fas Ligand) Antibody (13-5911-82) in Flow

Staining of mouse Fas Ligand-transfected L5178Y cells with 0.125 µg of Armenian Hamster IgG Isotype Control Biotin (Product # 13-4888-81) (open histogram) or 0.125 µg of Anti-Mouse CD178 (Fas Ligand) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

CD178 (Fas Ligand) Antibody in Western Blot (WB)

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Product Details

13-5911-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 11 publications 11 publications

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MFL3

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466794

Product Specific Information

Description: The MFL3 monoclonal antibody reacts with mouse Fas (CD95) Ligand, a 40 kDa type II transmembrane glycoprotein. FasL is a member of the TNF family and is expressed by mouse activated T cells. The interaction of FasL with its receptor CD95 induces Fas-mediated killing. It has been reported that the human FasL antigen is cleaved from the surface by matrix metalloproteinases (MMPs), resulting in a 26 kDa soluble form. The degree of sensitivity for the mouse antigen to MMPs has not been reported.

Applications Reported: The MFL3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The MFL3 antibody has been tested by flow cytometric analysis of mouse FasL transfected cells and activated T cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD178 (Fas ligand, FasL) is a type-II-membrane protein, whose N-terminus is in the cytoplasm and its C-terminal region extends into the extracellular space. Its receptor, FasR, is a cell-surface-type-I-membrane protein and a member of the tumor necrosis factor (TNF) and nerve growth factor (NGF) receptor family. As a member of the TNF-cytokine family CD178 induces apoptosis when interacting with FasR. CD178 may exist as either membrane bound (45 kD) or soluble forms (26 kD). The soluble protein can be released from cells upon cleavage by metalloproteinases. Binding of CD178 to Fas leads to oligomerization of the receptor and triggers apoptotic cell death through the interaction of other proteins. CD178 is predominantly expressed in activated T-lymphocytes and natural killer (NK) cells also it is expressed in the tissues of immune-privilege sites such as testis and eye. CD178 expression is also reported in various tissues as thymus, liver, ovary, lung, heart and kidney. It is assumed that induction of apoptosis through CD178 is predominantly involved in anti-viral immune responses. CD178 is a cell surface molecule belonging to the tumor necrosis factor family, binds to its receptor Fas, thus inducing apoptosis. Various cells express FAS, where CD178 is expressed predominantly on activated T cells. FAS and CD178 are involved in the down-regulation of immune reactions as well as T cell-mediated cytotoxicity. CD178 concentration has also been shown to be associated with atherosclerosis and inflammatory disease, in patients with hypertension. The Fas/ CD178 system has been shown to play a role in a number of human diseases, for example AIDS, hepatitis or cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CD4⁺ CTLs Act as a Key Effector Population for Allograft Rejection of MSCs in a Donor MHC-II Dependent Manner in Injured Liver.

Aging and disease

Shen S,Li Y,Jin M,Fan D,Pan R,Lin A,Chen Y,Xiang L,Zhao RC,Shao J

Published figure using CD178 (Fas Ligand) monoclonal antibody (Product # 13-5911-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2022

A macrophage-T cell coculture model for severe tissue injury-induced T cell death.

STAR protocols

Zhu J,Cao J,Liesz A,Roth S

Published figure using CD178 (Fas Ligand) monoclonal antibody (Product # 13-5911-82) in Flow Cytometry

Fri Dec 17 00:00:00 EST 2021

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD178 (Fas Ligand) monoclonal antibody (Product # 13-5911-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Hypoxia-Inducible Factor-2α Limits Natural Killer T Cell Cytotoxicity in Renal Ischemia/Reperfusion Injury.

Journal of the American Society of Nephrology : JASN

Zhang J,Han C,Dai H,Hou J,Dong Y,Cui X,Xu L,Zhang M,Xia Q

Published figure using CD178 (Fas Ligand) monoclonal antibody (Product # 13-5911-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

Hypoxia-Inducible Factor-2α Limits Natural Killer T Cell Cytotoxicity in Renal Ischemia/Reperfusion Injury.

Journal of the American Society of Nephrology : JASN

Zhang J,Han C,Dai H,Hou J,Dong Y,Cui X,Xu L,Zhang M,Xia Q

Published figure using CD178 (Fas Ligand) monoclonal antibody (Product # 13-5911-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

Lymphopenia associated with highly virulent H5N1 virus infection due to plasmacytoid dendritic cell-mediated apoptosis of T cells.

Journal of immunology (Baltimore, Md. : 1950)

Boonnak K,Vogel L,Feldmann F,Feldmann H,Legge KL,Subbarao K

13-5911 was used in Flow cytometry/Cell sorting to examine the mechanism(s) by which lethal H5N1 viruses cause lymphopenia in mammalian hosts.

Sun Jun 15 00:00:00 EDT 2014

Cellular-FLIP, Raji isoform (c-FLIP R) modulates cell death induction upon T-cell activation and infection.

European journal of immunology

Telieps T,Ewald F,Gereke M,Annemann M,Rauter Y,Schuster M,Ueffing N,von Smolinski D,Gruber AD,Bruder D,Schmitz I

13-5911 was used in Flow cytometry/Cell sorting to study the induction of endogenous murine cellular-FLIPR in activated lymphocytes.

Sat Jun 01 00:00:00 EDT 2013

Kupffer cells potentiate liver sinusoidal endothelial cell injury in sepsis by ligating programmed cell death ligand-1.

Journal of leukocyte biology

Hutchins NA,Wang F,Wang Y,Chung CS,Ayala A

13-5911 was used in Flow cytometry/Cell sorting to study Kupffer cell-mediated potentiation of liver sinusoidal endothelial cell injury in sepsis by ligating programmed cell death ligand-1.

Fri Nov 01 00:00:00 EDT 2013

Induction of rapid apoptosis for class I MHC molecule-restricted CD8(+) HIV-1 gp160-specific murine activated CTLs by free antigenic peptide in vivo.

International immunology

Nakagawa Y,Shimizu M,Norose Y,Takahashi M,Takahashi H

13-5911 was used in Flow cytometry/Cell sorting to provide evidence that exposure to free antigenic peptide leads to the rapid apoptosis of activated virus-specific CD8+ CTLs during infection in vivo.

Tue Jan 01 00:00:00 EST 2013

The magnitude of the T cell response to a clinically significant dose of influenza virus is regulated by TRAIL.

Journal of immunology (Baltimore, Md. : 1950)

Brincks EL,Gurung P,Langlois RA,Hemann EA,Legge KL,Griffith TS

13-5911 was used in Flow cytometry/Cell sorting to study how TRAIL affects the T cell response and ultimately influences the outcome of viral infection.

Tue Nov 01 00:00:00 EDT 2011

NKG2A inhibits invariant NKT cell activation in hepatic injury.

Journal of immunology (Baltimore, Md. : 1950)

Kawamura T,Takeda K,Kaneda H,Matsumoto H,Hayakawa Y,Raulet DH,Ikarashi Y,Kronenberg M,Yagita H,Kinoshita K,Abo T,Okumura K,Smyth MJ

13-5911 was used in Flow cytometry/Cell sorting to investigate the role of NK cell receptors in iNKT cell activation, showing that NKG2A-mediated signalling negatively regulated iNKT activation and hepatic injury.

Thu Jan 01 00:00:00 EST 2009

NKG2A inhibits invariant NKT cell activation in hepatic injury.

Journal of immunology (Baltimore, Md. : 1950)

Kawamura T,Takeda K,Kaneda H,Matsumoto H,Hayakawa Y,Raulet DH,Ikarashi Y,Kronenberg M,Yagita H,Kinoshita K,Abo T,Okumura K,Smyth MJ

Thu Jan 01 00:00:00 EST 2009

CD8 T cells utilize TRAIL to control influenza virus infection.

Journal of immunology (Baltimore, Md. : 1950)

Brincks EL,Katewa A,Kucaba TA,Griffith TS,Legge KL

13-5911 was used in Flow cytometry/Cell sorting to determine that TRAIL deficiency decreases CD8(+) T cell-mediated cytotoxicity.

Wed Oct 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: Apoptosis (APO 1) antigen ligand 1; CD178; CD178 antigen; CD95 ligand; CD95 ligand protein;Generalized lymphoproliferative disease (Gld); CD95-L; Fas antigen ligand; Fas ligand; Fas-LG; generalized lymphoproliferative disease; soluble form; TNFL; Tumor necrosis factor (ligand) superfamily member 6; Tumor necrosis factor ligand; Tumor necrosis factor ligand superfamily member 6; Tumor necrosis factor ligand superfamily member 6 (TNFL6 / TNFSF6)

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Gene Aliases: APT1LG1; CD178; CD95-L; CD95L; Fas-L; Fasl; Faslg; gld; Tnfsf6

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UniProt ID: (Mouse) P41047

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Entrez Gene ID: (Mouse) 14103

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Function(s)

death receptor binding cytokine activity tumor necrosis factor receptor binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process inflammatory cell apoptotic process immune response positive regulation of cell proliferation extrinsic apoptotic signaling pathway via death domain receptors negative regulation of angiogenesis cellular chloride ion homeostasis positive regulation of apoptotic process positive regulation of neuron apoptotic process positive regulation of epidermal growth factor receptor signaling pathway retinal cell programmed cell death endosomal lumen acidification T cell apoptotic process necroptotic process apoptotic signaling pathway extrinsic apoptotic signaling pathway necroptotic signaling pathway positive regulation of endothelial cell apoptotic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD178 (Fas Ligand) Monoclonal Antibody (MFL3), Biotin, eBioscience™

CD178 (Fas Ligand) Antibody (13-5911-82) in Flow

Product Details

13-5911-82

Western Blot (WB)

Flow Cytometry (Flow)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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