Invitrogen

MHC Class I (H-2Kd) Monoclonal Antibody (SF1-1.1.1), Biotin, eBioscience™

9 Published Figures

7 References

View all (11) MHC Class I (H-2Kd) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (9)

MHC Class I (H-2Kd) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 10

MHC Class I (H-2Kd) Antibody (13-5957-82) in Flow

Staining of BALB/c (left) and C57BL/6 (right) splenocytes with 0.125 µg of Mouse IgG2a K Isotype Control Biotin (Product # 13-4724-85) (blue histogram) or 0.125 µg of Anti-Mouse MHC Class I (H-2Kd) Biotin (purple histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

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Product Details

13-5957-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 7 publications 7 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

SF1-1.1.1

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1582234

Product Specific Information

Description: This SF1-1.1.1 monoclonal antibody reacts with the H-2Kd MHC class I alloantigen. H-2Kd is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. b, j, k, p, q, s, and v) has not been observed.

Applications Reported: This SF1-1.1.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This SF1-1.1.1 antibody has been tested by flow cytometric analysis of Balb/c splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Patient-derived xenograft culture-transplant system for investigation of human breast cancer metastasis.

Communications biology

Ma D,Hernandez GA,Lefebvre AEYT,Alshetaiwi H,Blake K,Dave KR,Rauf M,Williams JW,Davis RT,Evans KT,Longworth A,Masoud MYG,Lee R,Edwards RA,Digman MA,Kessenbrock K,Lawson DA

Published figure using MHC Class I (H-2Kd) monoclonal antibody (Product # 13-5957-82) in Flow Cytometry

Fri Nov 05 00:00:00 EDT 2021

Multipotency of mouse trophoblast stem cells.

Stem cell research & therapy

Hou M,Han J,Li G,Kwon MY,Jiang J,Emani S,Taglauer ES,Park JA,Choi EB,Vodnala M,Fong YW,Emani SM,Rosas IO,Perrella MA,Liu X

Published figure using MHC Class I (H-2Kd) monoclonal antibody (Product # 13-5957-82) in Flow Cytometry

Thu Feb 13 00:00:00 EST 2020

Targeting photodynamic and photothermal therapy to the endoplasmic reticulum enhances immunogenic cancer cell death.

Nature communications

Li W,Yang J,Luo L,Jiang M,Qin B,Yin H,Zhu C,Yuan X,Zhang J,Luo Z,Du Y,Li Q,Lou Y,Qiu Y,You J

Published figure using MHC Class I (H-2Kd) monoclonal antibody (Product # 13-5957-82) in Flow Cytometry

Fri Jul 26 00:00:00 EDT 2019

Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.

Cell

Wroblewska A,Dhainaut M,Ben-Zvi B,Rose SA,Park ES,Amir ED,Bektesevic A,Baccarini A,Merad M,Rahman AH,Brown BD

13-5957-82 was used in Flow Cytometry to show that combinatorial arrangements of linear epitopes can be used to generate a protein barcoding system which is capable of overcoming many limitations of current pooled screening technology.

Thu Nov 01 00:00:00 EDT 2018

Quiescent Tissue Stem Cells Evade Immune Surveillance.

Immunity

Agudo J,Park ES,Rose SA,Alibo E,Sweeney R,Dhainaut M,Kobayashi KS,Sachidanandam R,Baccarini A,Merad M,Brown BD

13-5957 was used in Flow cytometry/Cell sorting to identify a link between stem cell quiescence, antigen presentation, and immune evasion.

Tue Feb 20 00:00:00 EST 2018

A therapeutic cancer vaccine against GL261 murine glioma.

Journal of translational medicine

Kindy MS,Yu J,Zhu H,Smith MT,Gattoni-Celli S

Published figure using MHC Class I (H-2Kd) monoclonal antibody (Product # 13-5957-82) in Flow Cytometry

Tue Jan 05 00:00:00 EST 2016

Dietary gluten increases natural killer cell cytotoxicity and cytokine secretion.

European journal of immunology

Larsen J,Dall M,Antvorskov JC,Weile C,Engkilde K,Josefsen K,Buschard K

Published figure using MHC Class I (H-2Kd) monoclonal antibody (Product # 13-5957-82) in Flow Cytometry

Wed Oct 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2K(B); H-2K(K); H-2K(Q); MGC184092; MGC7052; MHC class I antigen; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-K transplantation antigen

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Gene Aliases: H-2K; H-2K(d); H2-K; H2-K1; K-f

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UniProt ID: (Mouse) P01901

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Entrez Gene ID: (Mouse) 14972

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Function(s)

receptor binding protein binding beta-2-microglobulin binding peptide binding peptide antigen binding T cell receptor binding poly(A) RNA binding TAP binding protein heterodimerization activity major histocompatibility complex protein

Process(es)

positive regulation of T cell mediated cytotoxicity immune system process antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent immune response antigen processing and presentation antigen processing and presentation of exogenous peptide antigen via MHC class I defense response to bacterium inner ear development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MHC Class I (H-2Kd) Monoclonal Antibody (SF1-1.1.1), Biotin, eBioscience™

MHC Class I (H-2Kd) Antibody (13-5957-82) in Flow

Product Details

13-5957-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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