Invitrogen

IL-5 Monoclonal Antibody (TRFK4), Biotin, eBioscience™

FIGURE: 1 / 1

IL-5 Antibody (13-7051-81) in IS

Naive mouse splenocytes (SJL/J strain) were stimulated with 2 µg/mL ConA in HL-1 media for 48 hours (right well) in the Mouse IL-5 ELISPOT Ready Set Go! (Product # 88-7825-88). Left well is medium alone control.

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Product Details

13-7051-81

Applications

Tested Dilution

Publications

ELISA (ELISA)

View 8 publications 8 publications

Immunostaining (IS)

0.5-2 µg/mL

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TRFK4

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466906

Product Specific Information

Description: The TRFK4 antibody reacts with mouse interleukin-5 (IL-5), also known as BCDF-alpha and eosinophil differentiation factor (EDF). This 15-20 kDa cytokine is secreted by Th2 cells and exists a homodimer.

Applications Reported: TRFK4 has been reported for use in detection of mouse IL-5 by ELISA.

Applications Tested: The TRFK4 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse Interleukin-5 (IL-5) in combination with the purified TRFK5 (14-7052) antibody for capture and recombinant mouse IL-5 (14-8051) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation.

Cell reports

Yan J,Pandey SP,Barnes BJ,Turner JR,Abraham C

13-7051 was used in an ELISA assay to identify a previously undefined key role for T cell-intrinsic IRF5. In mice, IRF5 in CD4+ T cells promotes Th1- and Th17-associated cytokines and decreases Th2-associated cytokines.

Tue Jun 30 00:00:00 EDT 2020

Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation.

The Journal of experimental medicine

Carpier JM,Zucchetti AE,Bataille L,Dogniaux S,Shafaq-Zadah M,Bardin S,Lucchino M,Maurin M,Joannas LD,Magalhaes JG,Johannes L,Galli T,Goud B,Hivroz C

13-7051 was used in ELISA to study the retrograde traffic of linker for activation of T cells from the plasma membrane to the Golgi-trans-Golgi network.

Mon Apr 02 00:00:00 EDT 2018

Intratracheal myriocin enhances allergen-induced Th2 inflammation and airway hyper-responsiveness.

Immunity, inflammation and disease

Edukulla R,Rehn KL,Liu B,McAlees JW,Hershey GK,Wang YH,Lewkowich I,Lindsley AW

13-7051 was used in an ELISA assay to evaluate the immune regulatory role of de novo ceramide generation in vitro and in vivo.

Thu Sep 01 00:00:00 EDT 2016

MHC class II-dependent basophil-CD4+ T cell interactions promote T(H)2 cytokine-dependent immunity.

Nature immunology

Perrigoue JG,Saenz SA,Siracusa MC,Allenspach EJ,Taylor BC,Giacomin PR,Nair MG,Du Y,Zaph C,van Rooijen N,Comeau MR,Pearce EJ,Laufer TM,Artis D

13-7051 was used in an ELISA assay to suggest that major histocompatibility complex class II-dependent interactions between basophils and CD4+ T cells promote T helper type 2 cytokine responses and immunity to helminth infection.

Wed Jul 01 00:00:00 EDT 2009

The essential role of single Ig IL-1 receptor-related molecule/Toll IL-1R8 in regulation of Th2 immune response.

Journal of immunology (Baltimore, Md. : 1950)

Bulek K,Swaidani S,Qin J,Lu Y,Gulen MF,Herjan T,Min B,Kastelein RA,Aronica M,Kosz-Vnenchak M,Li X

13-7051 was used in an ELISpot assay to investigate the role that SIGIRR/TIR8 plays in the regulation of Th2 immune responses.

Sun Mar 01 00:00:00 EST 2009

Differential role of gamma interferon in inhibiting pulmonary eosinophilia and exacerbating systemic disease in fusion protein-immunized mice undergoing challenge infection with respiratory syncytial virus.

Journal of virology

Castilow EM,Olson MR,Meyerholz DK,Varga SM

13-7051 was used in an ELISpot assay to compare the immune response elicited by the G versus F protein of the respiratory syncytial virus, showing a differential role of gamma interferon.

Sat Mar 01 00:00:00 EST 2008

CD8 T cells inhibit respiratory syncytial virus (RSV) vaccine-enhanced disease.

Journal of immunology (Baltimore, Md. : 1950)

Olson MR,Varga SM

13-7051-81 was used in ELISA to suggest that the failure of the FI-RSV vaccine to induce a CD8 T cell response may have contributed to the development of pulmonary eosinophilia and augmented disease that occurred in vaccinated individuals.

Mon Oct 15 00:00:00 EDT 2007

Pathogenic role for virus-specific CD4 T cells in mice with coronavirus-induced acute encephalitis.

The American journal of pathology

Anghelina D,Pewe L,Perlman S

13-7051 was used in an ELISpot assay to show that the host response to a single JHMV-specific immunodominant CD4 T-cell epitope is critical for severe disease.

Sat Jul 01 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: B-cell growth factor II; BCGF-II; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; IL-5; ILN; Interleukin; Interleukin-5; Interleukin5; T-cell replacing factor; TRF

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Gene Aliases: Il-5; Il5

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UniProt ID: (Mouse) P04401

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Entrez Gene ID: (Mouse) 16191

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Function(s)

cytokine activity interleukin-5 receptor binding growth factor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-5 Monoclonal Antibody (TRFK4), Biotin, eBioscience™

IL-5 Antibody (13-7051-81) in IS

Product Details

13-7051-81

ELISA (ELISA)

Immunostaining (IS)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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