Invitrogen

CCL2 (MCP-1) Monoclonal Antibody (2H5), Biotin, eBioscience™

9 Published Figures

11 References

View all (67) CCL2 (MCP-1) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (9)

CCL2 (MCP-1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 10

CCL2 (MCP-1) Antibody (13-7096-85) in Flow

Staining of mouse thiogylcolate induced peritoneal exudate cells (PECs) stimulated with LPS in the presence of Brefeldin A for 24 hours. with Anti-mouse F4/80 FITC (Product # 11-4801-82) followed by intracellular staining with Rat IgG2a kappa Isotype Control PE (Product # 12-4321-82) (left) or Anti-Mouse CCL2 PE.

CCL2 (MCP-1) Antibody in Western Blot (WB)

CCL2 (MCP-1) Antibody in Immunocytochemistry (ICC/IF)

CCL2 (MCP-1) Antibody in Immunohistochemistry (IHC)

CCL2 (MCP-1) Antibody in Neutralization (Neu)

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Product Details

13-7096-85

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

View 1 publication 1 publication

ELISA (ELISA)

0.5-2 µg/mL

View 6 publications 6 publications

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2H5

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466915

Product Specific Information

Description: The 2H5 monoclonal antibody reacts with mouse, rat, and human monocyte chemoattractant protein-1 (MCP-1), also known as CCL2 and MCAF.

Applications Reported: The 2H5 antibody has been reported for use in ELISA, intracellular staining for flow cytometric analysis, and cytokine neutralization. (Please use Functional Grade purified 2H5, Product # 16-7096, in functional assays).

Applications Tested: Mouse MCP-1 ELISA: The biotinylated 2H5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse MCP-1 in combination with the affinity purified 4E2/MCP (Product # 14-7091-81) antibody for capture and recombinant mouse MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.

Human MCP-1 ELISA: The biotinylated 2H5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human MCP-1 in combination with the affinity purified 5D3-F7 (Product # 14-7099-81) antibody for capture and recombinant human MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.

The Functional Grade purified 2H5 antibody has been tested for neutralization of MCP-1 bioactivity.The fluorochrome-conjugated 2H5 antibody has been tested for intracellular staining and flow cytometric analysis of mouse, rat, and human cells and can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mural cell-derived chemokines provide a protective niche to safeguard vascular macrophages and limit chronic inflammation.

Immunity

Pekayvaz K,Gold C,Hoseinpour P,Engel A,Martinez-Navarro A,Eivers L,Coletti R,Joppich M,Dionísio F,Kaiser R,Tomas L,Janjic A,Knott M,Mehari F,Polewka V,Kirschner M,Boda A,Nicolai L,Schulz H,Titova A,Kilani B,Lorenz M,Fingerle-Rowson G,Bucala R,Enard W,Zimmer R,Weber C,Libby P,Schulz C,Massberg S,Stark K

Published figure using CCL2 (MCP-1) monoclonal antibody (Product # 13-7096-81) in Neutralization

Tue Oct 10 00:00:00 EDT 2023

Apoptotic vesicles restore liver macrophage homeostasis to counteract type 2 diabetes.

Journal of extracellular vesicles

Zheng C,Sui B,Zhang X,Hu J,Chen J,Liu J,Wu D,Ye Q,Xiang L,Qiu X,Liu S,Deng Z,Zhou J,Liu S,Shi S,Jin Y

Published figure using CCL2 (MCP-1) monoclonal antibody (Product # 13-7096-81) in Immunocytochemistry

Sat May 01 00:00:00 EDT 2021

Apoptotic vesicles restore liver macrophage homeostasis to counteract type 2 diabetes.

Journal of extracellular vesicles

Zheng C,Sui B,Zhang X,Hu J,Chen J,Liu J,Wu D,Ye Q,Xiang L,Qiu X,Liu S,Deng Z,Zhou J,Liu S,Shi S,Jin Y

Published figure using CCL2 (MCP-1) monoclonal antibody (Product # 13-7096-81) in Immunocytochemistry

Sat May 01 00:00:00 EDT 2021

Cholangiocyte-Derived Exosomal lncRNA H19 Promotes Macrophage Activation and Hepatic Inflammation under Cholestatic Conditions.

Cells

Li X,Liu R,Wang Y,Zhu W,Zhao D,Wang X,Yang H,Gurley EC,Chen W,Hylemon PB,Zhou H

Published figure using CCL2 (MCP-1) monoclonal antibody (Product # 13-7096-81) in Western Blot

Sat Jan 11 00:00:00 EST 2020

Aggregation and Its Influence on the Immunomodulatory Activity of Synthetic Innate Defense Regulator Peptides.

Cell chemical biology

Haney EF,Wu BC,Lee K,Hilchie AL,Hancock REW

13-7096-85 was used in ELISA to examine the effect of aggregation on the immunomodulatory activity of a synthetic innate defense regulator peptide, 1018 (VRLIVAVRIWRR-NH2).

Thu Aug 17 00:00:00 EDT 2017

Development and validation of an immunosensor for monocyte chemotactic protein 1 using a silicon photonic microring resonator biosensing platform.

Clinical biochemistry

Valera E,Shia WW,Bailey RC

13-7096 was used in an ELISA assay to develop a silicon photonic immunosensor for the detection of clinically-relevant concentrations of monocyte chemotactic protein 1.

Fri Jan 01 00:00:00 EST 2016

Magnetically-actuated, bead-enhanced silicon photonic immunosensor.

Analytical methods : advancing methods and applications

Valera E,McClellan MS,Bailey RC

13-7096-85 was used in ELISA to demonstrate that addition of magnetic actuation into bead-enhanced sandwich assays on a silicon photonic biosensor platform might improve biomarker detection.

Wed Oct 21 00:00:00 EDT 2015

Conditioned medium from umbilical cord mesenchymal stem cells induces migration and angiogenesis.

Molecular medicine reports

Shen C,Lie P,Miao T,Yu M,Lu Q,Feng T,Li J,Zu T,Liu X,Li H

Published figure using CCL2 (MCP-1) monoclonal antibody (Product # 13-7096-81) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2015

Oxidized low-density lipoprotein induces long-term proinflammatory cytokine production and foam cell formation via epigenetic reprogramming of monocytes.

Arteriosclerosis, thrombosis, and vascular biology

Bekkering S,Quintin J,Joosten LA,van der Meer JW,Netea MG,Riksen NP

13-7096 was used in an ELISA assay to study the role of oxidised low-density lipoprotein (oxLDL) in inducing a long lasting proinflammatory phenotype.

Fri Aug 01 00:00:00 EDT 2014

The liver X receptor agonist GW3965 improves recovery from mild repetitive traumatic brain injury in mice partly through apolipoprotein E.

PloS one

Namjoshi DR,Martin G,Donkin J,Wilkinson A,Stukas S,Fan J,Carr M,Tabarestani S,Wuerth K,Hancock RE,Wellington CL

13-7096 was used in an ELISA assay to investigate the therapeutic use of Liver X receptor for treatment of TBI.

Tue Jul 09 00:00:00 EDT 2013

CCR2 defines a distinct population of NK cells and mediates their migration during influenza virus infection in mice.

PloS one

van Helden MJ,Zaiss DM,Sijts AJ

13-7096 was used in an ELISA assay to determine the role of CCR2 in NK cell migration during intranasal influenza virus infection, showing that it defines a distinct population of NK cells and mediates their migration.

Mon Jun 10 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: C-C motif chemokine 2; chemokine (C-C motif) ligand 2; H-MCP-1; HC11; immediate-early serum-responsive JE protein; Immediate-early serum-responsive protein JE; MCAF; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; Platelet-derived growth factor-inducible protein JE; RP23-350G1.3; small inducible cytokine A2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible gene JE; Small-inducible cytokine A2

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Gene Aliases: AI323594; CCL2; GDCF-2; HC11; HSMCR30; JE; MCAF; MCP-1; MCP1; SCYA2; Sigje; SMC-CF

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UniProt ID: (Human) P13500, (Rat) P14844, (Mouse) P10148

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Entrez Gene ID: (Human) 6347, (Rat) 24770, (Mouse) 20296

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Function(s)

protein kinase activity receptor binding chemokine activity heparin binding CCR2 chemokine receptor binding G-protein coupled receptor binding cytokine activity protein binding cytokine intercellular signal molecule

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CCL2 (MCP-1) Monoclonal Antibody (2H5), Biotin, eBioscience™

CCL2 (MCP-1) Antibody (13-7096-85) in Flow

Product Details

13-7096-85

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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