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Invitrogen
CD1d Monoclonal Antibody (51.1), eBioscience™
FIGURE: 1 / 9
CD1d Antibody (14-0016-82) in Flow
Staining of normal human peripheral blood cells with 0.5 µg of Mouse IgG2b K Isotype Control Purified (Product # 14-4732-82) (open histogram) or Anti-Human CD1d Purified (filled histogram) followed by Anti-Mouse IgG Biotin (Product # 13-4013-85) and Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.
Product Details
14-0016-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
51.1
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_837139
Product Specific Information
Description: The monoclonal antibody 51.1 reacts with human CD1d, a member of the CD1 family with similarity to the non-polymorphic MHC Class I-like molecules. CD1d is a highly conserved single transmembrane receptor of the Immunoglobulin Superfamily. CD1d can associate with beta-microglobulin another feature showing similarity to MHC class I molecules, but can also exist as a nonglycosylated protein not in association with beta microglobulin. This suggests different control mechanisms for presenting glycolipid containing molecules to CD1d reactive NKT cells. Expression of CD1d is found on B cells of the periphery, in resting monocytes and cortical thymocytes. On intestinal epithelial cells (IEC) expression is polarized. Expression can also be found at low levels intracellularly in hepatocytes. In HCV (hepatitis C virus) livers, CD1d is highly expressed compared to normal controls.
The 51.1 monoclonal antibody has been shown to have functional activity; blocking the interaction of CD1d transfected cells with NKT cells.
Applications Reported: This 51.1 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections.
Applications Tested: This 51.1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD1d is a heavy chain associated with Beta 2 microglobulin on cortical thymocytes. Beta 2 microglobulin independent expression of CD1d has also been demonstrated on human intestinal epithelial cells. CD1d is the sole group 2 member of the CD1 family of major histocompatibility (MHC) like glycoproteins. The CD1d gene encodes a divergent member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by the CD1d gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail. Diseases associated with CD1D include Mycobacterium Malmoense and Autoimmune Disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Antigen-presenting glycoprotein CD1d; CD1d; CD1D antigen, d polypeptide; differentiation antigen CD1-alpha-3; HMC class I antigen-like glycoprotein CD1D; R3G1; T-cell surface glycoprotein CD1d; thymocyte antigen CD1D
Gene Aliases: CD1A; CD1D; R3; R3G1
UniProt ID: (Human) P15813
Entrez Gene ID: (Human) 912
viral process
detection of bacterium
heterotypic cell-cell adhesion
positive regulation of T cell proliferation
T cell selection
innate immune response
positive regulation of innate immune response
antigen processing and presentation, endogenous lipid antigen via MHC class Ib
antigen processing and presentation, exogenous lipid antigen via MHC class Ib
regulation of immune response
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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