Invitrogen

CD2 Monoclonal Antibody (RM2-5), eBioscience™

FIGURE: 1 / 2

CD2 Antibody (14-0021-85) in Flow

Staining of mouse splenocytes with Anti-Mouse CD2 FITC. Autofluorescence is indicated by open histogram. Total viable cells were used for analysis.

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Product Details

14-0021-85

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Flow Cytometry (Flow)

0.25 µg/test

View 8 publications 8 publications

Neutralization (Neu)

Assay-Dependent

View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, lambda

Class

Monoclonal

Type

Antibody

Clone

RM2-5

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467042

Product Specific Information

Description: The RM2-5 monoclonal antibody reacts with the mouse CD2 molecule, an approximately 50-55 kDa cell surface receptor expressed by all mouse lymphocytes. Expression of the CD2 antigen in the mouse differs from that of the human in that B lineage cells in the mouse from the pre-B cell stage to mature B cells express CD2. CD2 is a ligand for CD48 in the mouse and is involved in adhesion and activation of T cells.

Applications Reported: The RM2-5 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. RM2-5 has also been reported in blocking of CD2 activity in in vitro functional assays. (Please use Functional Grade purified RM2-5, Product # 16-0021, in functional assays).

Applications Tested: The RM2-5 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD2 (LFA-2) is a monomeric surface antigen (MW range 45-58 kDa) of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells. CD2 is one of the earliest T-cell markers, being present on more than 95% of thymocytes and it is also found on some natural killer cells, but not on B lymphocytes. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it. The interaction between CD2 and CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection. Diseases associated with CD2 dysfunction include penis squamous cell carcinoma and immune deficiency due to the absence of the thymus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Multiple Levels of Control Determine How E4bp4/Nfil3 Regulates NK Cell Development.

Journal of immunology (Baltimore, Md. : 1950)

Kostrzewski T,Borg AJ,Meng Y,Filipovic I,Male V,Wack A,DiMaggio PA,Brady HJM

14-0021 was used in Flow cytometry/Cell sorting to reveal mechanisms in NK cell development and that E4bp4 is a central hub to process extrinsic stimuli.

Thu Feb 15 00:00:00 EST 2018

LNK/SH2B3 regulates IL-7 receptor signaling in normal and malignant B-progenitors.

The Journal of clinical investigation

Cheng Y,Chikwava K,Wu C,Zhang H,Bhagat A,Pei D,Choi JK,Tong W

14-0021 was used in Flow cytometry/Cell sorting to investigate how LNK regulates normal B cell development or promotes leukemogenesis.

Fri Apr 01 00:00:00 EDT 2016

Overexpression of RhoH Permits to Bypass the Pre-TCR Checkpoint.

PloS one

Tamehiro N,Oda H,Shirai M,Suzuki H

14-0021 was used in Flow cytometry/Cell sorting to indicate that an excess amount of RhoH is able to initiate pre-TCR signalling in the absence of pre-TCR complexes.

Mon Apr 25 00:00:00 EDT 2016

The transcription factor E4bp4/Nfil3 controls commitment to the NK lineage and directly regulates Eomes and Id2 expression.

The Journal of experimental medicine

Male V,Nisoli I,Kostrzewski T,Allan DS,Carlyle JR,Lord GM,Wack A,Brady HJ

14-0021 was used in Flow cytometry/Cell sorting to propose that E4bp4 is required for commitment to the NK lineage and can promote NK development by directly regulating the expression of the downstream transcription factors Eomes and Id2.

Mon Apr 07 00:00:00 EDT 2014

HDL and Glut1 inhibition reverse a hypermetabolic state in mouse models of myeloproliferative disorders.

The Journal of experimental medicine

Gautier EL,Westerterp M,Bhagwat N,Cremers S,Shih A,Abdel-Wahab O,Lütjohann D,Randolph GJ,Levine RL,Tall AR,Yvan-Charvet L

14-0021 was used in Flow cytometry/Cell sorting to investigate the underlying mechanisms leading to high metabolic rate in myeloproliferative disorders, showing that HDL and Glut1 inhibition reverse hypermetabolic state.

Mon Feb 11 00:00:00 EST 2013

Ligation of the CD2 co-stimulatory receptor enhances IL-2 production from first-generation chimeric antigen receptor T cells.

Gene therapy

Cheadle EJ,Rothwell DG,Bridgeman JS,Sheard VE,Hawkins RE,Gilham DE

14-0021 was used in Blocking experiments to demonstrate that signalling through endogenous CD2 following ligation drives IL-2 production by first-generation CD19-specific chimeric antigen receptor T-cells.

Thu Nov 01 00:00:00 EDT 2012

Ligation of the CD2 co-stimulatory receptor enhances IL-2 production from first-generation chimeric antigen receptor T cells.

Gene therapy

Cheadle EJ,Rothwell DG,Bridgeman JS,Sheard VE,Hawkins RE,Gilham DE

Thu Nov 01 00:00:00 EDT 2012

Rescue of tumor-infiltrating lymphocytes from activation-induced cell death enhances the antitumor CTL response in CD5-deficient mice.

Journal of immunology (Baltimore, Md. : 1950)

Tabbekh M,Franciszkiewicz K,Haouas H,Lécluse Y,Benihoud K,Raman C,Mami-Chouaib F

14-0021-85 was used in Flow Cytometry to compare the T cell response to the B16F10 melanoma engrafted into CD5-deficient and wild-type C57BL/6 mice.

Fri Jul 01 00:00:00 EDT 2011

S1P3 confers differential S1P-induced migration by autoreactive and non-autoreactive immature B cells and is required for normal B-cell development.

European journal of immunology

Donovan EE,Pelanda R,Torres RM

14-0021 was used in Flow cytometry/Cell sorting to asses whether sphingosine 1-phosphate contributes to B-cell development, egress and positioning within the bone marrow.

Mon Mar 01 00:00:00 EST 2010

Distinct roles of phosphoinositide-3 kinase and phospholipase Cgamma2 in B-cell receptor-mediated signal transduction.

Molecular and cellular biology

Dai X,Chen Y,Schuman J,Hua Z,Adamson JW,Wen R,Wang D

Published figure using CD2 monoclonal antibody (Product # 14-0021-85) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: CD2; FLJ46032; LFA-2; LFA-3 receptor; Ly-37; Lymphocyte antigen 37; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5

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Gene Aliases: Cd2; LFA-2; Ly-37; Ly37

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UniProt ID: (Mouse) P08920

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Entrez Gene ID: (Mouse) 12481

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Function(s)

glycoprotein binding antigen binding receptor activity receptor binding protein binding protein kinase binding receptor tyrosine kinase binding protein homodimerization activity protein self-association immunoglobulin receptor superfamily

Process(es)

cell adhesion single organismal cell-cell adhesion positive regulation of tumor necrosis factor production heterotypic cell-cell adhesion T cell activation positive regulation of interferon-gamma secretion positive regulation of interleukin-8 secretion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD2 Monoclonal Antibody (RM2-5), eBioscience™

CD2 Antibody (14-0021-85) in Flow

Product Details

14-0021-85

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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