Invitrogen

CD45RA Monoclonal Antibody (HI100), eBioscience™

CD45RA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 10

CD45RA Antibody (14-0458-82) in IHC (P)

Immunohistochemical analysis of CD45RA was performed using formalin-fixed paraffin-embedded human tonsil tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minutes. Fo... View More

CD45RA Antibody in Flow Cytometry (Flow)

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Product Details

14-0458-82

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 26 publications 26 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

HI100

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467272

Product Specific Information

Description: The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ peripheral T lymphocytes, CD8+ peripheral T lymphocytes, and B cells. The CD45RA+ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.

Applications Reported: The HI100 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.

Applications Tested: The HI100 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing and these isoforms differ in their extracellular domains, however, they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage, with the exception of mature red cells and is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Diseases associated with CD45 dysfunction include severe combined immunodeficiency and Hepatitis C.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Hedgehog-induced ZFYVE21 promotes chronic vascular inflammation by activating NLRP3 inflammasomes in T cells.

Science signaling

Jiang B,Wang S,Song G,Jiang Q,Fan M,Fang C,Li X,Soh CL,Manes TD,Cheru N,Qin L,Ren P,Jortner B,Wang Q,Quaranta E,Yoo P,Geirsson A,Davis RP,Tellides G,Pober JS,Jane-Wit D

14-0458-82 was used in Flow cytometry/Cell sorting to identify a T cell-autonomous role for ZFYVE21 in promoting chronic vascular inflammation associated with allograft vasculopathy.

Tue Mar 21 00:00:00 EDT 2023

A ZFYVE21-Rubicon-RNF34 signaling complex promotes endosome-associated inflammasome activity in endothelial cells.

Nature communications

Li X,Jiang Q,Song G,Barkestani MN,Wang Q,Wang S,Fan M,Fang C,Jiang B,Johnson J,Geirsson A,Tellides G,Pober JS,Jane-Wit D

14-0458-82 was used in Flow Cytometry to identify a protein complex modulating inflammasome activity on endosomes.

Wed May 24 00:00:00 EDT 2023

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

The Journal of experimental medicine

Louka E,Povinelli B,Rodriguez-Meira A,Buck G,Wen WX,Wang G,Sousos N,Ashley N,Hamblin A,Booth CAG,Roy A,Elliott N,Iskander D,de la Fuente J,Fordham N,O'Byrne S,Inglott S,Norfo R,Salio M,Thongjuea S,Rao A,Roberts I,Mead AJ

Published figure using CD45RA monoclonal antibody (Product # 14-0458-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

ZEB1 promotes pathogenic Th1 and Th17 cell differentiation in multiple sclerosis.

Cell reports

Qian Y,Arellano G,Ifergan I,Lin J,Snowden C,Kim T,Thomas JJ,Law C,Guan T,Balabanov RD,Kaech SM,Miller SD,Choi J

14-0458-82 was used in Flow Cytometry to identify disease-relevant regulators of T cell fate by examining mutations that modify risk for multiple sclerosis.

Tue Aug 24 00:00:00 EDT 2021

Differences in Maturation Status and Immune Phenotypes of Circulating Helios⁺ and Helios^- Tregs and Their Disrupted Correlations With Monocyte Subsets in Autoantibody-Positive T1D Individuals.

Frontiers in immunology

Zhang Y,Zhang J,Shi Y,Shen M,Lv H,Chen S,Feng Y,Chen H,Xu X,Yang T,Xu K

14-0458-82 was used in Flow Cytometry to explore the immunological changes in circulating Helios+ and Helios- regulator T-cells in type 1 diabetes.

Wed Sep 15 00:00:00 EDT 2021

Differences in Maturation Status and Immune Phenotypes of Circulating Helios⁺ and Helios^- Tregs and Their Disrupted Correlations With Monocyte Subsets in Autoantibody-Positive T1D Individuals.

Frontiers in immunology

Zhang Y,Zhang J,Shi Y,Shen M,Lv H,Chen S,Feng Y,Chen H,Xu X,Yang T,Xu K

14-0458-82 was used in Flow Cytometry to explore the immunological changes in circulating Helios+ and Helios- regulator T-cells in type 1 diabetes.

Wed Sep 15 00:00:00 EDT 2021

Single-Cell Analyses Reveal Megakaryocyte-Biased Hematopoiesis in Myelofibrosis and Identify Mutant Clone-Specific Targets.

Molecular cell

Psaila B,Wang G,Rodriguez-Meira A,Li R,Heuston EF,Murphy L,Yee D,Hitchcock IS,Sousos N,O'Sullivan J,Anderson S,Senis YA,Weinberg OK,Calicchio ML,Iskander D,Royston D,Milojkovic D,Roberts I,Bodine DM,Thongjuea S,Mead AJ

Published figure using CD45RA monoclonal antibody (Product # 14-0458-82) in Flow Cytometry

Thu May 07 00:00:00 EDT 2020

Phase I Study of Ficlatuzumab and Cetuximab in Cetuximab-Resistant, Recurrent/Metastatic Head and Neck Cancer.

Cancers

Bauman JE,Ohr J,Gooding WE,Ferris RL,Duvvuri U,Kim S,Johnson JT,Soloff AC,Wallweber G,Winslow J,Gaither-Davis A,Grandis JR,Stabile LP

14-0458-82 was used in Flow Cytometry to establish the recommended Phase II dose (RP2D). Secondary objectives included overall response rate (ORR), progression-free survival (PFS), and overall survival (OS).

Thu Jun 11 00:00:00 EDT 2020

The downregulated membrane expression of CD18 in CD34⁺ cells defines a primitive population of human hematopoietic stem cells.

Stem cell research & therapy

Mesa-Núñez C,Leon-Rico D,Aldea M,Damián C,Sanchez-Baltasar R,Sanchez R,Alberquilla O,Segovia JC,Bueren JA,Almarza E

Published figure using CD45RA monoclonal antibody (Product # 14-0458-82) in Flow Cytometry

Tue Apr 28 00:00:00 EDT 2020

MicroRNA‑155 inhibits the proliferation of CD8+ T cells via upregulating regulatory T cells in vitiligo.

Molecular medicine reports

Lv M,Li Z,Liu J,Lin F,Zhang Q,Li Z,Wang Y,Wang K,Xu Y

Published figure using CD45RA monoclonal antibody (Product # 14-0458-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

Intracellular delivery of mRNA to human primary T cells with microfluidic vortex shedding.

Scientific reports

Jarrell JA,Twite AA,Lau KHWJ,Kashani MN,Lievano AA,Acevedo J,Priest C,Nieva J,Gottlieb D,Pawell RS

Published figure using CD45RA monoclonal antibody (Product # 14-0458-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2019

Targeting enhancer switching overcomes non-genetic drug resistance in acute myeloid leukaemia.

Nature communications

Bell CC,Fennell KA,Chan YC,Rambow F,Yeung MM,Vassiliadis D,Lara L,Yeh P,Martelotto LG,Rogiers A,Kremer BE,Barbash O,Mohammad HP,Johanson TM,Burr ML,Dhar A,Karpinich N,Tian L,Tyler DS,MacPherson L,Shi J,Pinnawala N,Yew Fong C,Papenfuss AT,Grimmond SM,Dawson SJ,Allan RS,Kruger RG,Vakoc CR,Goode DL,Naik SH,Gilan O,Lam EYN,Marine JC,Prinjha RK,Dawson MA

Published figure using CD45RA monoclonal antibody (Product # 14-0458-82) in Flow Cytometry

Thu Jun 20 00:00:00 EDT 2019

Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection.

Nature

Eyquem J,Mansilla-Soto J,Giavridis T,van der Stegen SJ,Hamieh M,Cunanan KM,Odak A,Gönen M,Sadelain M

Published figure using CD45RA monoclonal antibody (Product # 14-0458-82) in Flow Cytometry

Thu Mar 02 00:00:00 EST 2017

The distribution and function of human memory T cell subsets in lung cancer.

Immunologic research

Sheng SY,Gu Y,Lu CG,Zou JY,Hong H,Wang R

Published figure using CD45RA monoclonal antibody (Product # 14-0458-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia.

Oncoimmunology

Catakovic K,Gassner FJ,Ratswohl C,Zaborsky N,Rebhandl S,Schubert M,Steiner M,Gutjahr JC,Pleyer L,Egle A,Hartmann TN,Greil R,Geisberger R

Published figure using CD45RA monoclonal antibody (Product # 14-0458-82) in Flow Cytometry

Sat Jan 29 00:00:00 EST 2022

Efficient gene disruption in cultured primary human endothelial cells by CRISPR/Cas9.

Circulation research

Abrahimi P,Chang WG,Kluger MS,Qyang Y,Tellides G,Saltzman WM,Pober JS

14-0458 was used in Flow cytometry/Cell sorting to demonstrate that the CRISPR/Cas9 modification of epithelial cells is a powerful platform for vascular research and for regenerative medicine.

Fri Jul 03 00:00:00 EDT 2015

Brief Report: IFIH1 Mutation Causes Systemic Lupus Erythematosus With Selective IgA Deficiency.

Arthritis & rheumatology (Hoboken, N.J.)

Van Eyck L,De Somer L,Pombal D,Bornschein S,Frans G,Humblet-Baron S,Moens L,de Zegher F,Bossuyt X,Wouters C,Liston A

14-0458 was used in Flow cytometry/Cell sorting to determine the underlying genetic defect responsible for severe early onset and refractory systemic lupus erythematosus.

Mon Jun 01 00:00:00 EDT 2015

Defining CD4 T cell memory by the epigenetic landscape of CpG DNA methylation.

Journal of immunology (Baltimore, Md. : 1950)

Komori HK,Hart T,LaMere SA,Chew PV,Salomon DR

14-0458 was used in Flow cytometry/Cell sorting to study differences in the methylation status of genes in memory and naive T cells.

Sun Feb 15 00:00:00 EST 2015

Autosomal dominant immune dysregulation syndrome in humans with CTLA4 mutations.

Nature medicine

Schubert D,Bode C,Kenefeck R,Hou TZ,Wing JB,Kennedy A,Bulashevska A,Petersen BS,Schäffer AA,Grüning BA,Unger S,Frede N,Baumann U,Witte T,Schmidt RE,Dueckers G,Niehues T,Seneviratne S,Kanariou M,Speckmann C,Ehl S,Rensing-Ehl A,Warnatz K,Rakhmanov M,Thimme R,Hasselblatt P,Emmerich F,Cathomen T,Backofen R,Fisch P,Seidl M,May A,Schmitt-Graeff A,Ikemizu S,Salzer U,Franke A,Sakaguchi S,Walker LSK,Sansom DM,Grimbacher B

14-0458 was used in Flow cytometry/Cell sorting to demonstrate the consequences for mice with mutations in CTLA4, an essential negative regulator of immune responses.

Mon Dec 01 00:00:00 EST 2014

PD-1(+) CD8(+) T cells are exhausted in tumours and functional in draining lymph nodes of colorectal cancer patients.

British journal of cancer

Wu X,Zhang H,Xing Q,Cui J,Li J,Li Y,Tan Y,Wang S

14-0458 was used in Flow cytometry/Cell sorting to evaluate the role of PD-1-PD-L1 pathway in CD8(+) T-cell functions in tumour-draining lymph nodes (TDLNs) and tumours of CRC patients.

Tue Sep 23 00:00:00 EDT 2014

Microbe-specific unconventional T cells induce human neutrophil differentiation into antigen cross-presenting cells.

Journal of immunology (Baltimore, Md. : 1950)

Davey MS,Morgan MP,Liuzzi AR,Tyler CJ,Khan MWA,Szakmany T,Hall JE,Moser B,Eberl M

14-0458 was used in Immunofluorescence to investigate how unconventional T cells represent key controllers of neutrophil-driven innate and adaptive responses to a broad range of pathogens.

Wed Oct 01 00:00:00 EDT 2014

Monocytes and γδ T cells control the acute-phase response to intravenous zoledronate: insights from a phase IV safety trial.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Welton JL,Morgan MP,Martí S,Stone MD,Moser B,Sewell AK,Turton J,Eberl M

14-0458 was used in Flow cytometry/Cell sorting to investigate the acute-phase response of otherwise healthy individuals to first-time intravenous treatment with aminobisphosphonates zoledronate.

Fri Mar 01 00:00:00 EST 2013

Helios+ and Helios- cells coexist within the natural FOXP3+ T regulatory cell subset in humans.

Journal of immunology (Baltimore, Md. : 1950)

Himmel ME,MacDonald KG,Garcia RV,Steiner TS,Levings MK

14-0458 was used in Flow cytometry/Cell sorting to investigate the link between Helios expression and natural regulatory FOXP3+ T cell subsets from different origins in humans.

Fri Mar 01 00:00:00 EST 2013

Novel serial positive enrichment technology enables clinical multiparameter cell sorting.

PloS one

Stemberger C,Dreher S,Tschulik C,Piossek C,Bet J,Yamamoto TN,Schiemann M,Neuenhahn M,Martin K,Schlapschy M,Skerra A,Schmidt T,Edinger M,Riddell SR,Germeroth L,Busch DH

14-0458 was used in Flow cytometry/Cell sorting to exhibit novel, low-affinity antibody-derived Fab-fragments for use in clinical cell preparations and research.

Mon Aug 27 00:00:00 EDT 2012

IL-15 transpresentation promotes both human T-cell reconstitution and T-cell-dependent antibody responses in vivo.

Proceedings of the National Academy of Sciences of the United States of America

Huntington ND,Alves NL,Legrand N,Lim A,Strick-Marchand H,Mention JJ,Plet A,Weijer K,Jacques Y,Becker PD,Guzman C,Soussan P,Kremsdorf D,Spits H,Di Santo JP

14-0458 was used in Flow cytometry/Cell sorting to investigate the role of transpresented IL-15 on human T-cell development and homeostasis.

Tue Apr 12 00:00:00 EDT 2011

Neutralizing IL-6 reduces human arterial allograft rejection by allowing emergence of CD161+ CD4+ regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Fogal B,Yi T,Wang C,Rao DA,Lebastchi A,Kulkarni S,Tellides G,Pober JS

14-0458 was used in Flow cytometry/Cell sorting to study the role of IL-6 in allogeneic T cell infiltration and intimal expansion in a model of human allograft rejection.

Thu Dec 15 00:00:00 EST 2011

Cytomegalovirus-infected human endothelial cells can stimulate allogeneic CD4+ memory T cells by releasing antigenic exosomes.

Journal of immunology (Baltimore, Md. : 1950)

Walker JD,Maier CL,Pober JS

14-0458 was used in Flow cytometry/Cell sorting to identify the mechanism by which CMV can exacerbate allograft rejection.

Sun Feb 01 00:00:00 EST 2009

PILAR is a novel modulator of human T-cell expansion.

Blood

Huarte E,Cubillos-Ruiz JR,Nesbeth YC,Scarlett UK,Martinez DG,Engle XA,Rigby WF,Pioli PA,Guyre PM,Conejo-Garcia JR

14-0458 was used in Flow cytometry/Cell sorting to identify a novel human transmembrane molecule expressed on T cells that enhances their expansion through CD161.

Fri Aug 15 00:00:00 EDT 2008

TCR-induced downregulation of protein tyrosine phosphatase PEST augments secondary T cell responses.

Molecular immunology

Arimura Y,Vang T,Tautz L,Williams S,Mustelin T

14-0458 was used in Magnetic cell separation to investigate how PTP-PEST is down-regulated following T cell activation, and how that can lead to reduced IL-2 secretion following TCR and CD28 stimulation.

Sun Jun 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T100; T200; T200 glycoprotein; T200 leukocyte common antigen

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD45RA Monoclonal Antibody (HI100), eBioscience™

CD45RA Antibody (14-0458-82) in IHC (P)

Product Details

14-0458-82

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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