Invitrogen

CD54 (ICAM-1) Monoclonal Antibody (HA58), eBioscience™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

10 Published Figures

20 References

View all (173) ICAM-1 (CD54) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD54 (ICAM-1) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

CD54 (ICAM-1) Antibody (14-0549-82) in ICC/IF

Theranostics 2017 - Figure 2 Cancer Stem Cell Traits of Differential Populations of CD54 + and CD54 - Cells. ( a ) FACS histograms show separate gating for the isolation of CD54 + and CD54 - PC3 and LNCaP cells. ( b ) Representative light micrograph fields and comparative quantification of the sphere-forming capacity of CD54 + and CD54 - LNCaP and PC3 cells. ( c ) Representative microscope fields from colony formation assays f... View More

CD54 (ICAM-1) Antibody in Immunohistochemistry (IHC)

CD54 (ICAM-1) Antibody in Western Blot (WB)

CD54 (ICAM-1) Antibody in Flow Cytometry (Flow)

CD54 (ICAM-1) Antibody in Flow Cytometry, Neutralization (Flow, Neu)

CD54 (ICAM-1) Antibody in ChIP Assay (ChIP)

CD54 (ICAM-1) Antibody in Functional Assay (Functional)

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Product Details

14-0549-82

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

View 2 publications 2 publications

Immunohistochemistry (IHC)

Assay-Dependent

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 9 publications 9 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Neutralization (Neu)

Assay-Dependent

View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

HA58

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467304

Product Specific Information

Description: The HA58 monoclonal antibody reacts with human CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligand CD11a is important in the inflammatory response.

Applications Reported: This HA58 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining. HA58 has also been reported in blocking of in vitro mixed lymphocyte reaction. (Please use Functional Grade purified HA58, Product # 16-0549, in functional assays).

Applications Tested: The HA58 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Elderberry extract improves molecular markers of endothelial dysfunction linked to atherosclerosis.

Food science & nutrition

Festa J,Hussain A,Hackney A,Desai U,Sahota TS,Singh H,Da Boit M

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0549-82) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2023

Impact of latency-reversing agents on human macrophage physiology.

Immunity, inflammation and disease

Hany L,Turmel MO,Barat C,Ouellet M,Tremblay MJ

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0549-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2023

Multimodal CRISPR perturbations of GWAS loci associated with coronary artery disease in vascular endothelial cells.

PLoS genetics

Wünnemann F,Fotsing Tadjo T,Beaudoin M,Lalonde S,Lo KS,Kleinstiver BP,Lettre G

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0549-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

Anti-Inflammatory Activity of Ferula assafoetida Oleo-Gum-Resin (Asafoetida) against TNF-α-Stimulated Human Umbilical Vein Endothelial Cells (HUVECs).

Mediators of inflammation

Mobasheri L,Khorashadizadeh M,Safarpour H,Mohammadi M,Anani Sarab G,Askari VR

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0549-82) in Flow Cytometry

Tue Sep 13 00:00:00 EDT 2022

Pomalidomide restores immune recognition of primary effusion lymphoma through upregulation of ICAM-1 and B7-2.

PLoS pathogens

Shrestha P,Davis DA,Jaeger HK,Stream A,Aisabor AI,Yarchoan R

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0549-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Pomalidomide restores immune recognition of primary effusion lymphoma through upregulation of ICAM-1 and B7-2.

PLoS pathogens

Shrestha P,Davis DA,Jaeger HK,Stream A,Aisabor AI,Yarchoan R

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0549-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Preservation of microvascular barrier function requires CD31 receptor-induced metabolic reprogramming.

Nature communications

Cheung KCP,Fanti S,Mauro C,Wang G,Nair AS,Fu H,Angeletti S,Spoto S,Fogolari M,Romano F,Aksentijevic D,Liu W,Li B,Cheng L,Jiang L,Vuononvirta J,Poobalasingam TR,Smith DM,Ciccozzi M,Solito E,Marelli-Berg FM

14-0549 was used in Western Blotting to show that EB recovery requires a CD31 receptor-induced, robust glycolytic response sustaining junction re-annealing.

Fri Jul 17 00:00:00 EDT 2020

Preservation of microvascular barrier function requires CD31 receptor-induced metabolic reprogramming.

Nature communications

14-0549 was used in Western Blotting to show that EB recovery requires a CD31 receptor-induced, robust glycolytic response sustaining junction re-annealing.

Fri Jul 17 00:00:00 EDT 2020

Preservation of microvascular barrier function requires CD31 receptor-induced metabolic reprogramming.

Nature communications

14-0549 was used in Western Blotting to show that EB recovery requires a CD31 receptor-induced, robust glycolytic response sustaining junction re-annealing.

Fri Jul 17 00:00:00 EDT 2020

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0549-82) in ChIP assay

Thu Jun 01 00:00:00 EDT 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0549-82) in ChIP assay

Thu Jun 01 00:00:00 EDT 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0549-82) in ChIP assay

Thu Jun 01 00:00:00 EDT 2017

CD54-NOTCH1 axis controls tumor initiation and cancer stem cell functions in human prostate cancer.

Theranostics

Li C,Liu S,Yan R,Han N,Wong KK,Li L

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0549-82) in Immunohistochemistry

Wed Oct 18 00:00:00 EDT 2017

CD54-NOTCH1 axis controls tumor initiation and cancer stem cell functions in human prostate cancer.

Theranostics

Li C,Liu S,Yan R,Han N,Wong KK,Li L

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0549-82) in Immunohistochemistry

Wed Oct 18 00:00:00 EDT 2017

Type I IFNs induce anti-tumor polarization of tumor associated neutrophils in mice and human.

International journal of cancer

Andzinski L,Kasnitz N,Stahnke S,Wu CF,Gereke M,von Köckritz-Blickwede M,Schilling B,Brandau S,Weiss S,Jablonska J

14054982 was used in flow cytometry to study the effects of IFN signaling on neutrophil polarization

Fri Apr 15 00:00:00 EDT 2016

A Quantitative Perspective on Surface Marker Selection for the Isolation of Functional Tumor Cells.

Breast cancer : basic and clinical research

Cahall CF,Lilly JL,Hirschowitz EA,Berron BJ

14-0549 was used in Western Blotting to extend expression of key tumor markers beyond traditional positive/negative immunohistochemical staining to guide future fluid biopsy targeting strategies.

Fri Aug 28 00:00:00 EDT 2015

Effect of nicotine and porphyromonas gingivalis lipopolysaccharide on endothelial cells in vitro.

PloS one

An N,Andrukhov O,Tang Y,Falkensammer F,Bantleon HP,Ouyang X,Rausch-Fan X

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0549-82) in Flow Cytometry

Mon Jun 29 00:00:00 EDT 2015

Minimal encounter time and separation determine ligand-receptor binding in cell adhesion.

Biophysical journal

Robert P,Nicolas A,Aranda-Espinoza S,Bongrand P,Limozin L

14-0549-82 was used in bead preparation to probe the hidden kinetics of ligand-receptor bond formation using single-molecule flow chamber assays and Brownian dynamics simulations.

Wed Jun 08 00:00:00 EDT 2011

Expression of endothelia and lymphocyte adhesion molecules in bronchus-associated lymphoid tissue (BALT) in adult human lung.

Respiratory research

Kawamata N,Xu B,Nishijima H,Aoyama K,Kusumoto M,Takeuchi T,Tei C,Michie SA,Matsuyama T

14-0549 was used in Immunohistochemistry to identify which adhesion molecules recruit specific subsets of lymphocytes into human bronchus-associated lymphoid tissue.

Thu Oct 22 00:00:00 EDT 2009

Tuning the formation and rupture of single ligand-receptor bonds by hyaluronan-induced repulsion.

Biophysical journal

Robert P,Sengupta K,Puech PH,Bongrand P,Limozin L

14-0549 was used in Flow cytometry/Cell sorting to open the way for further quantitative studies of the effect of molecular environment and separation distance on ligand-receptor association and dissociation.

Wed Oct 01 00:00:00 EDT 2008

The human natural killer cell immune synapse.

Proceedings of the National Academy of Sciences of the United States of America

Davis DM,Chiu I,Fassett M,Cohen GB,Mandelboim O,Strominger JL

Tue Dec 21 00:00:00 EST 1999

Bioinformatics

Protein Aliases: CD54; cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; Ly 47; Major group rhinovirus receptor; MALA2; sCD54; soluble CD54; Surface antigen of activated B cells

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Gene Aliases: BB2; CD54; ICAM1; P3.58

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UniProt ID: (Human) P05362

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Entrez Gene ID: (Human) 3383

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Function(s)

virus receptor activity receptor activity transmembrane signaling receptor activity integrin binding protein binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD54 (ICAM-1) Monoclonal Antibody (HA58), eBioscience™

CD54 (ICAM-1) Antibody (14-0549-82) in ICC/IF

Product Details

14-0549-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ChIP assay (ChIP)

Neutralization (Neu)

Functional Assay (Functional)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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