Invitrogen

CD135 (Flt3) Monoclonal Antibody (A2F10), eBioscience™

CD135 (Flt3) Antibody in Flow Cytometry (Flow)

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CD135 (Flt3) Antibody (14-1351-82) in Flow

Nature communications 2020 - Fig. 7 Lgr4/Gpr48 is involved in CML stem cell self-renewal in vivo. a RNA-Seq determinations of mRNA levels of the indicated GPCR family genes in LT-CML stem cells isolated from Gdpd3 +/+ tet-CML-affected mice (22 males, 12 females) and Gdpd3 -/- tet-CML-affected mice (five males, five females). Results are expressed as FPKM (see Methods). Data are the mean FPKM +- s.d. ( n = 3 biologically independent sam... View More

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Product Details

14-1351-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 16 publications 16 publications

Immunoprecipitation (IP)

Assay-Dependent

Mass Spectrometry (MS)

- -

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

A2F10

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467481

Product Specific Information

Description: The A2F10 monoclonal antibody reacts with mouse CD135, also known as Flk2/Flt3. A member of the tyrosine kinase receptor family, this 135-150 kDa molecule is expressed by primitive progenitor cells in fetal liver and adult bone marrow. Two-color staining of adult mouse bone marrow cells with A2F10 and RA3-6B2 (CD45R/B220) or M1/70 (CD11b) reveals Flk-2-positive subpopulations of B cells or myeloid cells, respectively.

Applications Reported: The A2F10 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation.

Applications Tested: The A2F10 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. (Fluorochrome-conjugated A2F10 is recommended for use in flow cytometry.).

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FLT3 is a tyrosine protein kinase that is a member of the growth factor receptor tyrosine kinase family. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

STS1 and STS2 Phosphatase Inhibitor Baicalein Enhances the Expansion of Hematopoietic and Progenitor Stem Cells and Alleviates 5-Fluorouracil-Induced Myelosuppression.

International journal of molecular sciences

Li N,Wang Y,Wang A,Zhang J,Jia C,Yu C,Song Z,Wang S,Liu L,Yi J,Bao Y,Huang Y,Sun L

Published figure using CD135 (Flt3) monoclonal antibody (Product # 14-1351-82) in Flow Cytometry

Fri Feb 03 00:00:00 EST 2023

Pseudo-mutant P53 is a unique phenotype of DNMT3A-mutated pre-leukemia.

Haematologica

Tuval A,Brilon Y,Azogy H,Moskovitz Y,Leshkowitz D,Salame TM,Minden MD,Tal P,Rotter V,Oren M,Kaushansky N,Shlush LI

14-1351-82 was used in Flow Cytometry to shed light upon a possible targetable P53 dysfunction in human preLHSPCs carrying DNMT3A mutations. This opens new avenues for leukemia prevention.

Tue Nov 01 00:00:00 EDT 2022

Aged hematopoietic stem cells are refractory to bloodborne systemic rejuvenation interventions.

The Journal of experimental medicine

Ho TT,Dellorusso PV,Verovskaya EV,Bakker ST,Flach J,Smith LK,Ventura PB,Lansinger OM,Hérault A,Zhang SY,Kang YA,Mitchell CA,Villeda SA,Passegué E

Published figure using CD135 (Flt3) monoclonal antibody (Product # 14-1351-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

Published figure using CD135 (Flt3) monoclonal antibody (Product # 14-1351-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

Zinc finger and BTB domain-containing protein 46 is essential for survival and proliferation of acute myeloid leukemia cell line but dispensable for normal hematopoiesis.

Chinese medical journal

Liu YY,Xiao FF,Yang BJ,Li X,Xu SN,Chen ZW,Li P,Huang YX,Fu XM,Huang XQ,Zheng GL,Chen JP,Hou Y

Published figure using CD135 (Flt3) monoclonal antibody (Product # 14-1351-82) in Flow Cytometry

Mon Jul 20 00:00:00 EDT 2020

The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Ochiai R,Matsubara E,Kondo C,Yang KM,Hoshii T,Araki M,Araki K,Sotomaru Y,Sasaki K,Mitani K,Kim DW,Ooshima A,Kim SJ

Published figure using CD135 (Flt3) monoclonal antibody (Product # 14-1351-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

An Id2^RFP-Reporter Mouse Redefines Innate Lymphoid Cell Precursor Potentials.

Immunity

Xu W,Cherrier DE,Chea S,Vosshenrich C,Serafini N,Petit M,Liu P,Golub R,Di Santo JP

Published figure using CD135 (Flt3) monoclonal antibody (Product # 14-1351-82) in Flow Cytometry

Tue Apr 16 00:00:00 EDT 2019

Polychromic Reporter Mice Reveal Unappreciated Innate Lymphoid Cell Progenitor Heterogeneity and Elusive ILC3 Progenitors in Bone Marrow.

Immunity

Walker JA,Clark PA,Crisp A,Barlow JL,Szeto A,Ferreira ACF,Rana BMJ,Jolin HE,Rodriguez-Rodriguez N,Sivasubramaniam M,Pannell R,Cruickshank J,Daly M,Haim-Vilmovsky L,Teichmann SA,McKenzie ANJ

Published figure using CD135 (Flt3) monoclonal antibody (Product # 14-1351-82) in Flow Cytometry

Tue Jul 16 00:00:00 EDT 2019

Jak1 Integrates Cytokine Sensing to Regulate Hematopoietic Stem Cell Function and Stress Hematopoiesis.

Cell stem cell

Kleppe M,Spitzer MH,Li S,Hill CE,Dong L,Papalexi E,De Groote S,Bowman RL,Keller M,Koppikar P,Rapaport FT,Teruya-Feldstein J,Gandara J,Mason CE,Nolan GP,Levine RL

14-1351 was used in Flow cytometry/Cell sorting to elucidate the specific functions of JAK1 in haematopoietic stem cell homeostasis and response to stress.

Thu Oct 05 00:00:00 EDT 2017

Osteopontin attenuates aging-associated phenotypes of hematopoietic stem cells.

The EMBO journal

Guidi N,Sacma M,Ständker L,Soller K,Marka G,Eiwen K,Weiss JM,Kirchhoff F,Weil T,Cancelas JA,Florian MC,Geiger H

14135182 was used in flow cytometry to examine the contribution of stroma-derived osteopontin for hematopoietic stem cell aging

Mon Apr 03 00:00:00 EDT 2017

A population of hematopoietic stem cells derives from GATA4-expressing progenitors located in the placenta and lateral mesoderm of mice.

Haematologica

Cañete A,Carmona R,Ariza L,Sánchez MJ,Rojas A,Muñoz-Chápuli R

14135182 was used in flow cytometry to utilize a G2-Gata4Cre;R26REYFP mouse line to track the developmental fate of the G2-Gata4 cell lineage

Sat Apr 01 00:00:00 EDT 2017

LCN2 overexpression in bone enhances the hematopoietic compartment via modulation of the bone marrow microenvironment.

Journal of cellular physiology

Costa D,Principi E,Lazzarini E,Descalzi F,Cancedda R,Castagnola P,Tavella S

14135182 was used in flow cytometry to examine the function of lipocalin-2 in bone tissue

Wed Nov 01 00:00:00 EDT 2017

PML-RARA requires DNA methyltransferase 3A to initiate acute promyelocytic leukemia.

The Journal of clinical investigation

Cole CB,Verdoni AM,Ketkar S,Leight ER,Russler-Germain DA,Lamprecht TL,Demeter RT,Magrini V,Ley TJ

14135182 was used in flow cytometry to study how DNMT3A is involved in the function of PML-RARA, RUNX1-RUNX1T1, and MLL-AF9

Fri Jan 01 00:00:00 EST 2016

GRK6 regulates ROS response and maintains hematopoietic stem cell self-renewal.

Cell death & disease

Le Q,Yao W,Chen Y,Yan B,Liu C,Yuan M,Zhou Y,Ma L

14135182 was used in flow cytometry to use knockout mice to determine if GRK6 contributes to hematopoiesis

Thu Nov 24 00:00:00 EST 2016

Mpath maps multi-branching single-cell trajectories revealing progenitor cell progression during development.

Nature communications

Chen J,Schlitzer A,Chakarov S,Ginhoux F,Poidinger M

14135182 was used in flow cytometry to describe an algorithm for constructing cell lineages from single-cell data

Thu Jun 30 00:00:00 EDT 2016

Evaluation of the long-term reconstituting subset of hematopoietic stem cells with CD150.

Stem cells (Dayton, Ohio)

Papathanasiou P,Attema JL,Karsunky H,Xu J,Smale ST,Weissman IL

14-1351 was used in Flow cytometry/Cell sorting to identify cell surface markers that allow the isolation and characterisation of haematopoietic stem cells.

Thu Oct 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CD135; Fetal liver kinase 2; FL cytokine receptor; FLK-2; FLT-3; Fms-like tyrosine kinase 3; OTTHUMP00000214829; Receptor-type tyrosine-protein kinase FLT3; RP11-153M24.3; tyrosine-protein kinase FLT3; Tyrosine-protein kinase receptor flk-2

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Gene Aliases: B230315G04; CD135; Flk-2; Flk2; Flt-3; Flt3; Ly72; wmfl

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UniProt ID: (Mouse) Q00342

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Entrez Gene ID: (Mouse) 14255

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Function(s)

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity cytokine receptor activity protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups ubiquitin protein ligase binding protein complex binding glucocorticoid receptor binding phosphatidylinositol 3-kinase binding transmembrane signal receptor

Process(es)

leukocyte homeostasis positive regulation of protein phosphorylation myeloid progenitor cell differentiation lymphoid progenitor cell differentiation pro-B cell differentiation pro-T cell differentiation protein phosphorylation transmembrane receptor protein tyrosine kinase signaling pathway negative regulation of cell proliferation phosphorylation peptidyl-tyrosine phosphorylation cytokine-mediated signaling pathway hemopoiesis lymphocyte differentiation cell differentiation B cell differentiation common myeloid progenitor cell proliferation negative regulation of B cell differentiation lymphocyte proliferation protein autophosphorylation cellular response to cytokine stimulus dendritic cell differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD135 (Flt3) Monoclonal Antibody (A2F10), eBioscience™

CD135 (Flt3) Antibody (14-1351-82) in Flow

Product Details

14-1351-82

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Mass Spectrometry (MS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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