Invitrogen

CD138 (Syndecan-1) Monoclonal Antibody (DL-101), eBioscience™

10 Published Figures

View all (80) CD138 (Syndecan-1) antibodies

Questions & Answers

Questions & Answers

Published Figures (10)

CD138 (Syndecan-1) Antibody in Immunocytochemistry (ICC/IF)

CD138 (Syndecan-1) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

CD138 (Syndecan-1) Antibody (14-1389-82) in ICC/IF

The Journal of clinical investigation 2015 - Published figure using CD138 (Syndecan-1) monoclonal antibody (Product # 14-1389-82) in Immunocytochemistry

CD138 (Syndecan-1) Antibody in Immunocytochemistry (ICC/IF)

CD138 (Syndecan-1) Antibody in Flow Cytometry (Flow)

CD138 (Syndecan-1) Antibody in Flow Cytometry (Flow)

CD138 (Syndecan-1) Antibody in Flow Cytometry (Flow)

CD138 (Syndecan-1) Antibody in Flow Cytometry (Flow)

CD138 (Syndecan-1) Antibody in Flow Cytometry (Flow)

CD138 (Syndecan-1) Antibody in Flow Cytometry (Flow)

CD138 (Syndecan-1) Antibody in Flow Cytometry (Flow)

CD138 (Syndecan-1) Antibody in Flow Cytometry (Flow)

CD138 (Syndecan-1) Antibody in Flow Cytometry (Flow)

Product Image Gallery

CD138 (Syndecan-1) Antibody in Immunocytochemistry (ICC/IF) — Published figure using CD138 (Syndecan-1) monoclonal antibody (Product # 14-1389-82) in Immunocytochemistry

CD138 (Syndecan-1) Antibody in Flow Cytometry (Flow) — Supernatant from PBMCs cultured with Dengue virus increases surface receptors for Dengue virus cell binding and entry as well as immune checkpoint inhibition on tumor cells. A Flow plot analysis of MDA-MB-231 tumor cells by flow cytometry for expression of Dengue virus receptor ligands, DC-SIGN and Syndecan-1 , as well as immune checkpoint receptor, PD-L1. B Fold change of receptors comparing levels from the PV001-DV supernatant treatment (DV sup) over media control (Control sup)

CD138 (Syndecan-1) Antibody in Flow Cytometry (Flow) — Figure 5. M-CSF macrophages drive KSHV-infected B cell differentiation into long-lived plasma cells (A-C) B cells co-cultured with M-CSF macrophages were infected with KSHV at MOI ~1. (A) Cells were assessed by flow cytometry for percentage of CD38++ plasmablasts (Q1) or CD138+ plasma cells (Q2+Q3). CD14+ cells (macrophages) were gated out to limit analyses to B cells ( Figure S3J ). Data are representative of 4 independent donor sample experiments (primary data in Figures S6A - S6F ). (B and C) Average percentage of CD38++ plasmablasts (B) or CD138+ plasma cells (C) at indicated day post-infection with M-CSF macrophage co-culture. Error bars indicate standard deviation. p values calculated using t test: one-tailed paired two sample for means. See also Figure S6 .

CD138 (Syndecan-1) Antibody in Flow Cytometry (Flow) — Fig 4 p16 INK4a is a functional barrier to EBV driven proliferation of lymphoblastoid cells. (A) Blueprint of the primary transcript and the spliced mRNA with the three exons of CDKN2A on chromosome 9 encoding the p16 INK4a protein. The target site of the RNP complex within the 1st exon (exon1alpha) (chr9:21,974,678-21,974,827) is shown. (B) Study of the biological effect of the CDKN2A knockout in a time course experiment. WT and p16 KO cells were mixed such that the fraction of the latter was in the order of 10 to 20%, when the cells were infected with WT or DeltaEBNA3C EBV strains. The knockout status of the CDKN2A gene was studied by next generation sequencing to analyze the CD46 locus of the mixed cell populations over time. The fraction of cells with a disabled CDKN2A gene increased in cells infected with DeltaEBNA3C EBV exceeding 80% after eight weeks, whereas the knockout status of CDKN2A in the population of cells infected with WT EBV did not show a clear trend. Results from two biological replicates are shown, additional replicates can be found in S4A Fig . (C) Cell numbers of four different B cell populations were plotted as a function of days post nucleofection (x-axis) versus the format of the cell culture vessel (y-axis) starting with a single well in a 48-well cluster plate. 2x10 6 B cells with an intact CDKN2A locus (WT cells) or cells with an edited CDKN2A gene (p16 KO cells) were infected with wild-type (WT) EBV (left panel) or DeltaEBNA3C EBV (right panel).

CD138 (Syndecan-1) Antibody in Flow Cytometry (Flow) — Published figure using CD138 (Syndecan-1) monoclonal antibody (Product # 14-1389-82) in Flow Cytometry

CD138 (Syndecan-1) Antibody in Flow Cytometry (Flow) — Published figure using CD138 (Syndecan-1) monoclonal antibody (Product # 14-1389-82) in Flow Cytometry

CD138 (Syndecan-1) Antibody in Flow Cytometry (Flow) — Published figure using CD138 (Syndecan-1) monoclonal antibody (Product # 14-1389-82) in Flow Cytometry

CD138 (Syndecan-1) Antibody in Flow Cytometry (Flow) — Published figure using CD138 (Syndecan-1) monoclonal antibody (Product # 14-1389-82) in Flow Cytometry

CD138 (Syndecan-1) Antibody in Flow Cytometry (Flow) — 10.1371/journal.pone.0167323.g002 Fig 2 Hypoxia increases CD138 low population and alters transcriptional profile of HMCLs. Cell were cultured at either normoxia (21% O 2 ) or hypoxia (1% O 2 ) for 3 days, labeled with CD138-APC antibody and the percentage of CD138 low and CD138 high cells were analyzed by flow cytometry. A. Representative dot plots of different HMCLs and B. quantification of % CD138 low fractions under normoxia or hypoxia. C. qRT-PCR analysis of SDC1 , stem cell genes ( NANOG , OCT4 ), and VEGF-A . For panels B and C, bars represent mean of three independent runs +- SEM. *p < 0.05 vs. normoxia.

CD138 (Syndecan-1) Antibody in Flow Cytometry (Flow) — 10.1371/journal.pone.0167323.g001 Fig 1 Flow cytometric analysis of stem-like cells in HMCLs. A. Representative dot plots for CD138 vs. side scatter and B. quantification of % CD138 low fractions. C. Hoechst 33342 staining for SP with or without verapamil. Gate represents the % SP fractions, MP = main population. D. Quantification of % SP cells in MM.1S and OPM-2 cell lines +- verapamil. Bars represent mean of three independent runs +- SEM, *p < 0.05 vs. control.

Product Details

14-1389-82

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 7 publications 7 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

DL-101

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467489

Product Specific Information

Description: The DL-101 monoclonal antibody reacts with human CD138, also known as syndecan-1. CD138 is a transmembrane protein containing chondroitin sulfate and heparin sulfate moieties responsible for binding to the extracellular matrix components. CD138 is not expressed by mature B cells but is present on pre-B cells and the finally differentiated plasma cells.

Applications Reported: DL-101 has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.

Applications Tested: The DL-101 antibody has been tested by flow cytometric analysis of cell lines. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Macrophages drive KSHV B cell latency.

Cell reports

Szymula A,Samayoa-Reyes G,Ogolla S,Liu B,Li S,George A,Van Sciver N,Rochford R,Simas JP,Kaye KM

Published figure using CD138 (Syndecan-1) monoclonal antibody (Product # 14-1389-82) in Flow Cytometry

Tue Jul 25 00:00:00 EDT 2023

Attenuated Dengue virus PV001-DV induces oncolytic tumor cell death and potent immune responses.

Journal of translational medicine

Goldufsky JW,Daniels P,Williams MD,Gupta K,Lyday B,Chen T,Singh G,Kaufman HL,Zloza A,Marzo AL

Published figure using CD138 (Syndecan-1) monoclonal antibody (Product # 14-1389-82) in Flow Cytometry

Wed Jul 19 00:00:00 EDT 2023

Highly efficient CRISPR-Cas9-mediated gene knockout in primary human B cells for functional genetic studies of Epstein-Barr virus infection.

PLoS pathogens

Akidil E,Albanese M,Buschle A,Ruhle A,Pich D,Keppler OT,Hammerschmidt W

Published figure using CD138 (Syndecan-1) monoclonal antibody (Product # 14-1389-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Peli1 negatively regulates noncanonical NF-κB signaling to restrain systemic lupus erythematosus.

Nature communications

Liu J,Huang X,Hao S,Wang Y,Liu M,Xu J,Zhang X,Yu T,Gan S,Dai D,Luo X,Lu Q,Mao C,Zhang Y,Shen N,Li B,Huang M,Zhu X,Jin J,Cheng X,Sun SC,Xiao Y

Published figure using CD138 (Syndecan-1) monoclonal antibody (Product # 14-1389-82) in Flow Cytometry

Mon Mar 19 00:00:00 EDT 2018

Mitochondrial-Targeted Decyl-Triphenylphosphonium Enhances 2-Deoxy-D-Glucose Mediated Oxidative Stress and Clonogenic Killing of Multiple Myeloma Cells.

PloS one

Schibler J,Tomanek-Chalkley AM,Reedy JL,Zhan F,Spitz DR,Schultz MK,Goel A

Published figure using CD138 (Syndecan-1) monoclonal antibody (Product # 14-1389-82) in Flow Cytometry

Thu Jul 06 00:00:00 EDT 2017

Phosphorylation-mediated EZH2 inactivation promotes drug resistance in multiple myeloma.

The Journal of clinical investigation

Kikuchi J,Koyama D,Wada T,Izumi T,Hofgaard PO,Bogen B,Furukawa Y

Published figure using CD138 (Syndecan-1) monoclonal antibody (Product # 14-1389-82) in Immunocytochemistry

Mon Oct 26 00:00:00 EDT 2015

Genome-wide siRNA screen reveals a new cellular partner of NK cell receptor KIR2DL4: heparan sulfate directly modulates KIR2DL4-mediated responses.

Journal of immunology (Baltimore, Md. : 1950)

Brusilovsky M,Cordoba M,Rosental B,Hershkovitz O,Andrake MD,Pecherskaya A,Einarson MB,Zhou Y,Braiman A,Campbell KS,Porgador A

14-1389 was used in Flow cytometry/Cell sorting to demonstrate that exogenous HS/heparin can regulate cytokine production by KIR2DL4-expressing NK cells and HEK293T cells.

Fri Nov 15 00:00:00 EST 2013

Syndecan-1 is involved in osteoprotegerin-induced chemotaxis in human peripheral blood monocytes.

The Journal of clinical endocrinology and metabolism

Mosheimer BA,Kaneider NC,Feistritzer C,Djanani AM,Sturn DH,Patsch JR,Wiedermann CJ

14-1389 was used in Flow cytometry/Cell sorting to study whether osteoprotegerin affects monocyte locomotion in vitro and the possible mechanisms and pathways involved.

Sun May 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: CD antigen 138; CD138; CD138 antigen; heparan sulfate proteoglycan fibroblast growth factor receptor; sCD138; soluble CD138; SYND1; syndecan proteoglycan 1; Syndecan-1; Syndecan1

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Gene Aliases: CD138; SDC; SDC1; SYND1; syndecan

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UniProt ID: (Human) P18827

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Entrez Gene ID: (Human) 6382

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glycoprotein binding protein binding protein C-terminus binding transmembrane signal receptor

Process(es)

retinoid metabolic process ureteric bud development glycosaminoglycan biosynthetic process glycosaminoglycan catabolic process inflammatory response response to toxic substance cell migration glycosaminoglycan metabolic process wound healing lipoprotein metabolic process odontogenesis response to hydrogen peroxide myoblast development response to glucocorticoid response to cAMP response to calcium ion striated muscle cell development Sertoli cell development canonical Wnt signaling pathway positive regulation of exosomal secretion positive regulation of extracellular exosome assembly

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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