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Invitrogen
CD157 Monoclonal Antibody (eBioSY11B5 (SY11B5)), eBioscience™
Product Details
14-1579-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
eBioSY11B5 (SY11B5)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_529487
Product Specific Information
Description: The eBioSY11B5 monoclonal antibody recognizes human CD157 (Mo5, BST-1). CD157 is a 42-45 kDa, GPI-anchored protein with structural and functional similarities with CD38. CD157 was initially cloned because of its expression on monocytes and macrophages, and was subsequently discovered to be the same protein named BST-1, discovered for its expression on bone marrow stromal cells and its ability to stimulate the proliferation of a mouse pre-B cell line. CD157 is a pleiotropic ectoenzyme and is thought to act independently as an enzyme and receptor. Similar to CD38, CD157 is involved in the metabolism of NAD+ and this activity may be involved in regulating intracellular Ca2+ levels. As a receptor, upon binding of its putative ligand, CD157 is thought to initiate a signal transduction cascade resulting in the phosphorylation of cytoplasmic proteins including focal adhesion kinase (FAK). The mechanism and functional significance of CD157-initiated signal transduction remain to be fully characterized.
Applications Reported: This eBioSY11B5 (SY11B5) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB) (non-reduced only), and immunohistology staining of frozen tissue sections.
Applications Tested: This eBioSY11B5 (SY11B5) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Bone marrow stromal cell antigen 1 (BST1) is a stromal cell line-derived glycosylphosphatidylinositol-anchored molecule that facilitates pre-B cell growth. BST1, which is also known as ADP-rybosyl cyclase 2 and DAMP-1, is an enzyme which is expressed by BST1 gene in human. BST1 expression is enhanced in bone marrow stromal cell lines derived from patients with rheumatoid arthritis. The polyclonal B cell abnormalities in rheumatoid arthritis may be, at least in part, attributed to BST1 overexpression in the stromal cell population.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ADP-ribosyl cyclase 2; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2; bone marrow stromal antigen 1; Bone marrow stromal cell antigen 1; BST-1; cADPR hydrolase 2; CD157; CD157 antigen; Cyclic ADP-ribose hydrolase 2; NAD(+) nucleosidase; NAD+ nucleosidase
Gene Aliases: BST1; CD157
UniProt ID: (Human) Q10588
Entrez Gene ID: (Human) 683
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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