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14-3-3 Polyclonal Antibody

14-3-3 Antibody in Immunocytochemistry (ICC/IF)

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14-3-3 Antibody (14503-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using YWHAQ antibody 14503-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

14-3-3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

14-3-3 Antibody in Flow Cytometry (Flow)

14-3-3 Antibody in Immunoprecipitation (IP)

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Product Details

14503-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

View 5 publications 5 publications

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

14-3-3 Fusion Protein Ag5959 (1-245 aa encoded by BC056867)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.31 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody was raised against full-length 14-3-3 theta.

Immunogen sequence: MEKTELIQK AKLAEQAERY DDMATCMKAV TEQGAELSNE ERNLLSVAYK NVVGGRRSAW RVISSIEQKT DTSDKKLQLI KDYREKVESE LRSICTTVLE LLDKYLIANA TNPESKVFYL KMKGDYFRYL AEVACGDDRK QTIDNSQGAY QEAFDISKKE MQPTHPIRLG LALNFSVFYY EILNNPELAC TLAKTAFDEA IAELDTLNED SYKDSTLIMQ LLRDNLTLWT SDSAGEECDA AEGAEN (1-245 aa encoded by B C056867)

Target Information

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy

Li Y,Chen J,Wang B,Xu Z,Wu C,Ma J,Song Q,Geng Q,Yu J,Pei H,Yao Y

Wed Mar 01 00:00:00 EST 2023

CircPAK1 promotes the progression of hepatocellular carcinoma via modulation of YAP nucleus localization by interacting with 14-3-3ζ.

Journal of experimental & clinical cancer research : CR

Hao X,Zhang Y,Shi X,Liu H,Zheng Z,Han G,Rong D,Zhang C,Tang W,Wang X

Thu Sep 22 00:00:00 EDT 2022

CircPAK1 promotes the progression of hepatocellular carcinoma via modulation of YAP nucleus localization by interacting with 14-3-3ζ.

Journal of experimental & clinical cancer research : CR

Hao X,Zhang Y,Shi X,Liu H,Zheng Z,Han G,Rong D,Zhang C,Tang W,Wang X

Thu Sep 22 00:00:00 EDT 2022

CircPAK1 promotes the progression of hepatocellular carcinoma via modulation of YAP nucleus localization by interacting with 14-3-3ζ.

Journal of experimental & clinical cancer research : CR

Hao X,Zhang Y,Shi X,Liu H,Zheng Z,Han G,Rong D,Zhang C,Tang W,Wang X

Thu Sep 22 00:00:00 EDT 2022

The interaction protein of SORBS2 in myocardial tissue to find out the pathogenic mechanism of LVNC disease.

Aging

Li C,Zheng Y,Liu Y,Jin GH,Pan H,Yin F,Wu J

Thu Jan 20 00:00:00 EST 2022

The interaction protein of SORBS2 in myocardial tissue to find out the pathogenic mechanism of LVNC disease.

Aging

Li C,Zheng Y,Liu Y,Jin GH,Pan H,Yin F,Wu J

Thu Jan 20 00:00:00 EST 2022

The interaction protein of SORBS2 in myocardial tissue to find out the pathogenic mechanism of LVNC disease.

Aging

Li C,Zheng Y,Liu Y,Jin GH,Pan H,Yin F,Wu J

Thu Jan 20 00:00:00 EST 2022

The interaction protein of SORBS2 in myocardial tissue to find out the pathogenic mechanism of LVNC disease.

Aging

Li C,Zheng Y,Liu Y,Jin GH,Pan H,Yin F,Wu J

Thu Jan 20 00:00:00 EST 2022

APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.

International journal of molecular sciences

Naki M,Gourdomichali O,Zonke K,Kattan FG,Makridakis M,Kontostathi G,Vlahou A,Doxakis E

Fri May 06 00:00:00 EDT 2022

HMGB1 signaling phosphorylates Ku70 and impairs DNA damage repair in Alzheimer's disease pathology.

Communications biology

Tanaka H,Kondo K,Fujita K,Homma H,Tagawa K,Jin X,Jin M,Yoshioka Y,Takayama S,Masuda H,Tokuyama R,Nakazaki Y,Saito T,Saido T,Murayama S,Ikura T,Ito N,Yamamori Y,Tomii K,Bianchi ME,Okazawa H

Mon Oct 11 00:00:00 EDT 2021

PADI6 Regulates Trophoblast Cell Migration-Invasion Through the Hippo/YAP1 Pathway in Hydatidiform Moles.

Journal of inflammation research

Huang B,Zhao Y,Zhou L,Gong T,Feng J,Han P,Qian J

Mon Apr 25 00:00:00 EDT 2022

Nedd4-2 haploinsufficiency in mice causes increased seizure susceptibility and impaired Kir4.1 ubiquitination.

Biochimica et biophysica acta. Molecular basis of disease

Liu X,Zhang H,Zhang B,Tu J,Li X,Zhao Y

Tue Jun 01 00:00:00 EDT 2021

14-3-3 proteins protect AMPK-phosphorylated ten-eleven translocation-2 (TET2) from PP2A-mediated dephosphorylation.

The Journal of biological chemistry

Kundu A,Shelar S,Ghosh AP,Ballestas M,Kirkman R,Nam H,Brinkley GJ,Karki S,Mobley JA,Bae S,Varambally S,Sudarshan S

Fri Feb 07 00:00:00 EST 2020

Ser/Thr kinase Trc controls neurite outgrowth in Drosophila by modulating microtubule-microtubule sliding.

eLife

Norkett R,Del Castillo U,Lu W,Gelfand VI

Wed Feb 05 00:00:00 EST 2020

Glucocorticoids and myosin5b loss of function induce heightened PKA signaling in addition to membrane traffic defects.

Molecular biology of the cell

Forteza R,Ahsan MK,Cartón-García F,Arango D,Ameen NA,Salas PJ

Sun Dec 15 00:00:00 EST 2019

Regulatory factor X5 promotes hepatocellular carcinoma progression by transactivating tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta and suppressing apoptosis.

Chinese medical journal

Chen DB,Zhao YJ,Wang XY,Liao WJ,Chen P,Deng KJ,Cong X,Fei R,Wu X,Shao QX,Wei L,Xie XW,Chen HS

Fri Jul 05 00:00:00 EDT 2019

Regulatory factor X5 promotes hepatocellular carcinoma progression by transactivating tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta and suppressing apoptosis.

Chinese medical journal

Chen DB,Zhao YJ,Wang XY,Liao WJ,Chen P,Deng KJ,Cong X,Fei R,Wu X,Shao QX,Wei L,Xie XW,Chen HS

Fri Jul 05 00:00:00 EDT 2019

Quantitative proteomic dissection of a native 14-3-3ε interacting protein complex associated with hepatocellular carcinoma.

Amino acids

Bai C,Tang S,Bai C,Chen X

Tue Apr 01 00:00:00 EDT 2014

Quantitative proteomic dissection of a native 14-3-3ε interacting protein complex associated with hepatocellular carcinoma.

Amino acids

Bai C,Tang S,Bai C,Chen X

Tue Apr 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: 14-3-3 protein T-cell; 14-3-3 protein tau; 14-3-3 protein theta; 14-3-3 tau; 14-3-3 theta; OTTHUMP00000165887; Protein HS1; protein tau; protein, theta; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide

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Gene Aliases: 14-3-3; 14-3-3t; 1C5; 2700028P07Rik; AA409740; AU021156; HS1; R74690; YWHAQ

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UniProt ID: (Human) P27348, (Rat) P68255, (Mouse) P68254

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Entrez Gene ID: (Human) 10971, (Rat) 25577, (Mouse) 22630

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Function(s)

protein binding protein C-terminus binding protein domain specific binding ion channel binding protein N-terminus binding 14-3-3 protein binding scaffold/adaptor protein

Process(es)

protein targeting small GTPase mediated signal transduction substantia nigra development negative regulation of ion transmembrane transport negative regulation of transcription, DNA-templated membrane organization positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway signal transduction

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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14-3-3 Polyclonal Antibody

14-3-3 Antibody (14503-1-AP) in ICC/IF

Product Details

14503-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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