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14-3-3 beta Antibody (MA5-28985) in WB
Western Blot using 14-3-3 beta Monoclonal Antibody (5) (Product # MA5-28985) at 1:500 dilution. Lane A: Hela Whole Cell Lysate, Lane B: HepG2 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Mouse IgG H&L (DyLight™ 800) at 1:15,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 32 kDa.
Product Details
MA5-28985
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Cynomolgus monkey
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
05
Immunogen
Recombinant Cynomolgus 14-3-3 beta/YWHAB protein (Met2-Asn244)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2784925
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Cynomolgus 14-3-3 beta/YWHAB.
Target Information
14-3-3, a family of acidic and soluble proteins, highly conserved in amino acid sequences from yeast to mammals, is expressed in all eukaryotic cells. Seven isoforms(beta, gamma, epsilon, eta, zeta, sigma and tau/theta) encoded by seven distinct genes are identified in mammals and forms homo- and hetero- dimeric cup-shaped structures. As 14-3-3 is interacted with more than 100 binding partners, it regulates key proteins involved in various biological processes such as signal trans-duction, cell cycle, transcriptional control, cell proliferation, apoptosis, and ion channel physiology. Most 14-3-3 requires phosphorylation of serine or threonine residues in the target sequence. This protein is abundantly expressed in the brain and has been detected in the cerebrospinal fluid of patients with different neurological disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 14-3-3 protein beta/alpha; hypothetical protein GW128; KCIP 1; KCIP1; RP1-148E22.1; unnamed protein product
Gene Aliases: GW128; QccE-16215; YWHAB
UniProt ID: (Cynomolgus monkey) Q4R572
Entrez Gene ID: (Cynomolgus monkey) 101926442
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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