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14-3-3 beta/zeta Antibody (PA5-104448) in ICC/IF
Immunofluorescent analysis of 14-3-3 beta/zeta in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and 14-3-3 beta/zeta polyclonal antibody (Product # PA5-104448) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG A... View More
Product Details
PA5-104448
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human YWHAZ(Accession P63104), corresponding to amino acid residues K158-E208.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2853749
Product Specific Information
Antibody detects endogenous levels of total 14-3-3 zeta/delta.
Target Information
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 14-3-3 alpha; 14-3-3 delta; 14-3-3 protein beta; 14-3-3 protein beta-subtype; 14-3-3 protein beta/alpha; 14-3-3 protein zeta/delta; 14-3-3 protein/cytosolic phospholipase A2; 14-3-3 zeta; 14-3-3d; 3-monooxygenase/tryptophan 5 monooxygenase activation protein beta polypeptide; brain protein 14-3-3, beta isoform; epididymis luminal protein 4; epididymis secretory protein Li 1; epididymis secretory protein Li 3; epididymis secretory protein Li 93; KCIP-1; Mitochondrial import stimulation factor S1 subunit; phospholipase A2; Prepronerve growth factor RNH-1; Protein 1054; Protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; SEZ-2; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta polypeptide; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein, beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5-monooxgenase activation protein, beta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein beta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, beta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide; YWHAE
Gene Aliases: 1110013I11Rik; 1300003C17Rik; 14-3-3-zeta; 14-3-3z; 14-3-3zeta; AI596267; AL022924; AU020854; GW128; HEL-S-1; HEL-S-3; HEL-S-93; HEL4; HS1; KCIP-1; Msfs1; YWHAA; YWHAB; YWHAD; YWHAZ
UniProt ID: (Human) P31946, (Human) P63104, (Mouse) Q9CQV8, (Rat) P35213, (Mouse) P63101, (Rat) P63102
Entrez Gene ID: (Human) 7529, (Human) 7534, (Mouse) 54401, (Rat) 56011, (Mouse) 22631, (Rat) 25578
transcription corepressor activity
protein binding
protein C-terminus binding
enzyme binding
protein domain specific binding
protein complex binding
histone deacetylase binding
phosphoserine binding
phosphoprotein binding
cadherin binding involved in cell-cell adhesion
transcription factor binding
protein kinase binding
ubiquitin protein ligase binding
identical protein binding
poly(A) RNA binding
scaffold/adaptor protein
MAPK cascade
protein targeting
viral process
negative regulation of protein dephosphorylation
hippo signaling
positive regulation of catalytic activity
regulation of mRNA stability
negative regulation of G-protein coupled receptor protein signaling pathway
negative regulation of transcription, DNA-templated
cytoplasmic sequestering of protein
protein heterooligomerization
membrane organization
cell-cell adhesion
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
signal transduction
platelet activation
negative regulation of apoptotic process
establishment of Golgi localization
Golgi reassembly
histamine secretion by mast cell
protein targeting to mitochondrion
regulation of cell death
response to drug
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