Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 6
14-3-3 epsilon Antibody (PA5-86411) in IHC (P)
Immunohistochemical analysis of 14-3-3 epsilon in paraffin-embedded human kidney carcinoma tissue showing cytoplasmic/nuclear staining and negative control (right). Sample was incubated with polyclonal 14-3-3 epsilon antibody (Product # PA5-86411) using a 1:50 dilution, and followed by goat anti-rabbit IgG-biotin and avidin-peroxidase secondary antibodies.
Product Details
PA5-86411
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide, corresponding to amino acids 200-250 of Human 14-3-3 epsilon.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2803189
Product Specific Information
Purity is > 95% by SDS-PAGE.
Target Information
14-3-3 epsilon belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 14-3-3 epsilon; 14-3-3 protein epsilon; 14-3-3E; epididymis luminal protein 2; mitochondrial import stimulation factor (MSF) L subunit; Mitochondrial import stimulation factor L subunit; MSF L; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activatioprotein epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activatioprotein, epsilon polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide
Gene Aliases: 14-3-3E; AU019196; HEL2; KCIP-1; MDCR; MDS; YWHAE
UniProt ID: (Human) P62258, (Rat) P62260, (Mouse) P62259
Entrez Gene ID: (Human) 7531, (Rat) 29753, (Mouse) 22627
protein binding
potassium channel regulator activity
enzyme binding
protein domain specific binding
MHC class II protein complex binding
ubiquitin protein ligase binding
histone deacetylase binding
ion channel binding
poly(A) RNA binding
protein heterodimerization activity
phosphoserine binding
phosphoprotein binding
cadherin binding involved in cell-cell adhesion
protein kinase C inhibitor activity
protein complex binding
scaffold/adaptor protein
transporter
G2/M transition of mitotic cell cycle
neuron migration
regulation of heart rate by hormone
protein targeting
viral process
substantia nigra development
hippocampus development
cerebral cortex development
hippo signaling
intracellular signal transduction
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
regulation of membrane repolarization
membrane organization
membrane repolarization during cardiac muscle cell action potential
regulation of heart rate by cardiac conduction
cell-cell adhesion
regulation of cellular response to heat
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
regulation of potassium ion transmembrane transporter activity
negative regulation of peptidyl-serine dephosphorylation
signal transduction
negative regulation of protein dephosphorylation
negative regulation of protein serine/threonine kinase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support