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14-3-3 eta Antibody (PA5-29774) in WB
Western Blot analysis of 14-3-3-eta was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a 14-3-3 eta Polyclonal Antibody (Product # PA5-29774) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Product Details
PA5-29774
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 246 of Human 14-3-3 eta
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2547248
Product Specific Information
Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7.
Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (83%), Xenopus laevis (91%), Rabbit (98%), Chicken (97%), Rhesus Monkey (100%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat has been associated with early-onset schizophrenia.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 14-3-3 eta; 14-3-3 protein eta; 1433F; Protein AS1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide; YWHA114-3-3 eta
Gene Aliases: YWHA1; YWHAH
UniProt ID: (Human) Q04917
Entrez Gene ID: (Human) 7533
regulation of sodium ion transport
glucocorticoid catabolic process
intracellular protein transport
substantia nigra development
glucocorticoid receptor signaling pathway
regulation of neuron differentiation
positive regulation of transcription, DNA-templated
regulation of synaptic plasticity
negative regulation of dendrite morphogenesis
membrane organization
membrane depolarization during action potential
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
regulation of sodium ion transmembrane transporter activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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