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14-3-3 sigma Antibody (PA5-86412) in IHC (P)
Immunohistochemical analysis of 14-3-3 sigma in paraffin-embedded human kidney carcinoma tissue showing cytoplasmic/nuclear staining and negative control (right). Sample was incubated with polyclonal 14-3-3 sigma antibody (Product # PA5-86412) using a 1:50 dilution, and followed by goat anti-rabbit IgG-biotin and avidin-peroxidase secondary antibodies.
Product Details
PA5-86412
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide, corresponding to amino acids 40-90 of Human 14-3-3 sigma.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2803190
Product Specific Information
Purity is > 95% by SDS-PAGE.
Target Information
This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 14-3-3 protein sigma; 14-3-3 sigma; 14-3-3 sigma protein; 14-3-3sigma; Epithelial cell marker protein 1; HGNC:10773; Stratifin; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, sigma polypeptide
Gene Aliases: Er; HME1; Mkrn3; Mme1; SFN; YWHAS
UniProt ID: (Human) P31947, (Mouse) O70456
Entrez Gene ID: (Human) 2810, (Mouse) 55948
regulation of cyclin-dependent protein serine/threonine kinase activity
release of cytochrome c from mitochondria
keratinocyte development
negative regulation of protein kinase activity
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
signal transduction
intrinsic apoptotic signaling pathway in response to DNA damage
regulation of epidermal cell division
negative regulation of keratinocyte proliferation
positive regulation of cell growth
keratinization
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of epidermal cell differentiation
positive regulation of protein export from nucleus
membrane organization
establishment of skin barrier
negative regulation of protein serine/threonine kinase activity
cell-cell adhesion
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
keratinocyte differentiation
skin development
regulation of cell cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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