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Invitrogen
14-3-3 theta Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
14-3-3 theta Antibody (PA5-18822) in ICC/IF
Immunofluorescence analysis of 14-3-3 theta was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with 14-3-3 theta Goat Polyclonal Antibody (Product # PA5-18822) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labele... View More
Product Details
PA5-18822
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Dog
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (DDRKQTIDNSQ) corresponding to the internal amino acids of YWHAQ
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_10984084
Product Specific Information
This antibody is predicted to react with canine, mouse and rat based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 4,000.
Target Information
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 14-3-3 protein T-cell; 14-3-3 protein tau; 14-3-3 protein theta; OTTHUMP00000165887; Protein HS1; protein tau; protein, theta; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
Gene Aliases: 14-3-3; 1C5; HS1; YWHAQ
UniProt ID: (Human) P27348
Entrez Gene ID: (Human) 10971
protein targeting
small GTPase mediated signal transduction
substantia nigra development
negative regulation of ion transmembrane transport
negative regulation of transcription, DNA-templated
membrane organization
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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