Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Connexin 43 Monoclonal Antibody (Connexin43 (1A)), eBioscience™
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
Connexin 43 Antibody (14-4759-82) in IHC (P)
Immunohistochemistry of frozen mouse heart using 5 µg/mL of Mouse IgG1 K Isotype Control Purified (left) or 5 µg/mL of Anti-Mouse Connexin 43 Purified (right), followed by 10 µg/mL of F (ab')2 Anti-Mouse IgG eFluor® 570. Nuclei are stained with DAPI.
Product Details
14-4759-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
Connexin43 (1A)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2572880
Product Specific Information
Description: The Connexin43 monoclonal antibody recognizes mouse Connexin 43. Connexins are a family of integral membrane proteins that form gap junction channels, which function to facilitate direct cell-cell communication. Gap junctions are comprised of two hemichannels, or connexons, which are made up of six connexin proteins. Over 20 connexin proteins have been identified and each combine to form either homomeric or heteromeric channels with different functional properties. Connexin 43 is expressed in many tissues, including the central nervous system, heart, and bone. Connexin 43 is the most abundant connexin found in the myocardium and is also the primary connexin expressed in astrocytes. Mutations in connexin 43 are associated with ocluodentodigital dysplasia, a rare developmental disorder characterized by craniofacial and limb abnormalities. Connexin 43 may also be a promising therapeutic target in the treatment of malignant melanomas and mammary gland tumors.
The monoclonal antibody Connexin43 also recognizes human and rat connexin 43.
Applications Reported: This Connexin43 antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, microscopy, and immunocytochemistry.
Applications Tested: This Connexin43 antibody has been tested by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication. GFAP are membrane-spanning proteins that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is also targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5. Mutations in the GFAP gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy, oculodentodigital dysplasia and heart malformations. Alternatively spliced transcript variants of GFAP have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha 1 connexin; connexin 43; Connexin-43; Cx-43; Cx43; CXA1; DFNB38; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; gap junction membrane channel protein alpha 1; gap junction protein, alpha 1, 43kDa; ODD; ODOD; SDTY3; Syndactyly type III
Gene Aliases: AU042049; AVSD3; AW546267; CMDR; Cnx43; connexin43; CX43; Cx43alpha1; Cxn-43; EKVP; Gja-1; GJA1; GJAL; HLHS1; HSS; Npm1; ODDD; PPKCA
UniProt ID: (Human) P17302, (Mouse) P23242, (Rat) P08050
Entrez Gene ID: (Human) 2697, (Mouse) 14609, (Rat) 24392
signal transducer activity
receptor binding
gap junction channel activity
protein binding
ion transmembrane transporter activity
SH3 domain binding
PDZ domain binding
beta-tubulin binding
connexin binding
gap junction channel activity involved in cardiac conduction electrical coupling
scaffold protein binding
gap junction channel activity involved in cell communication by electrical coupling
protein domain specific binding
transmembrane transporter activity
gap junction
osteoblast differentiation
in utero embryonic development
neuron migration
negative regulation of endothelial cell proliferation
heart looping
regulation of heart rate
epithelial cell maturation
lens development in camera-type eye
chronic inflammatory response
positive regulation of glomerular filtration
endothelium development
atrial ventricular junction remodeling
transport
apoptotic process
muscle contraction
signal transduction
positive regulation of cytosolic calcium ion concentration
cell-cell signaling
heart development
adult heart development
response to pH
response to glucose
vascular transport
positive regulation of gene expression
negative regulation of gene expression
cell communication by chemical coupling
cell communication by electrical coupling
positive regulation of cell communication by chemical coupling
ATP transport
gap junction assembly
negative regulation of cell growth
regulation of bone mineralization
positive regulation of insulin secretion
ion transmembrane transport
response to fluid shear stress
embryonic digit morphogenesis
positive regulation of I-kappaB kinase/NF-kappaB signaling
skeletal muscle tissue regeneration
response to peptide hormone
positive regulation of osteoblast differentiation
positive regulation of protein catabolic process
positive regulation of striated muscle tissue development
positive regulation of vasoconstriction
positive regulation of vasodilation
regulation of bone remodeling
blood vessel morphogenesis
neuron projection morphogenesis
protein oligomerization
regulation of calcium ion transport
negative regulation of cardiac muscle cell proliferation
milk ejection
regulation of ventricular cardiac muscle cell membrane repolarization
regulation of atrial cardiac muscle cell membrane depolarization
regulation of ventricular cardiac muscle cell membrane depolarization
negative regulation of wound healing
cellular response to mechanical stimulus
atrial cardiac muscle cell action potential
cell communication by electrical coupling involved in cardiac conduction
negative regulation of DNA biosynthetic process
regulation of bicellular tight junction assembly
positive regulation of behavioral fear response
epicardial cell to mesenchymal cell transition
cell communication
regulation of heart contraction
negative regulation of cell proliferation
embryonic heart tube development
cell-cell junction organization
transmembrane transport
regulation of blood vessel remodeling
cardiac conduction
establishment of epithelial cell polarity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support