Invitrogen

GL7 Monoclonal Antibody (GL-7 (GL7)), eBioscience™

FIGURE: 1 / 15

GL7 Antibody (14-5902-82) in Flow

Staining of 3-day unstimulated (left) and 3-day Con A-stimulated (Product # 00-4978-03) (right) BALB/c splenocytes with 0.25 µg of Rat IgM K Isotype Control Purified (Product # 14-4341-82) (open histogram) or 0.25 µg of Anti-Human/Mouse GL7 (T and B Cell Activation Marker) Purified (filled histogram) followed by Anti-Rat IgM PE (Product # 12-0990-82).Total viable cells were used for analysis.

GL7 Antibody in Immunocytochemistry (ICC/IF)

GL7 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

14-5902-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

0.5 µg/test

View 23 publications 23 publications

Immunoprecipitation (IP)

Assay-Dependent

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgM

Class

Monoclonal

Type

Antibody

Clone

GL-7 (GL7)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467715

Product Specific Information

Description: This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22.

Applications Reported: The GL-7 (GL7) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections.

Applications Tested: The GL-7 (GL7) antibody has been tested by flow cytometric analysis of Con A-stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

GL7 is a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Microbiota-Derived Metabolites Suppress Arthritis by Amplifying Aryl-Hydrocarbon Receptor Activation in Regulatory B Cells.

Cell metabolism

Rosser EC,Piper CJM,Matei DE,Blair PA,Rendeiro AF,Orford M,Alber DG,Krausgruber T,Catalan D,Klein N,Manson JJ,Drozdov I,Bock C,Wedderburn LR,Eaton S,Mauri C

Published figure using GL7 monoclonal antibody (Product # 14-5902-82) in Immunohistochemistry

Tue Apr 07 00:00:00 EDT 2020

Microbiota-Derived Metabolites Suppress Arthritis by Amplifying Aryl-Hydrocarbon Receptor Activation in Regulatory B Cells.

Cell metabolism

Rosser EC,Piper CJM,Matei DE,Blair PA,Rendeiro AF,Orford M,Alber DG,Krausgruber T,Catalan D,Klein N,Manson JJ,Drozdov I,Bock C,Wedderburn LR,Eaton S,Mauri C

Published figure using GL7 monoclonal antibody (Product # 14-5902-82) in Immunohistochemistry

Tue Apr 07 00:00:00 EDT 2020

αv Integrins regulate germinal center B cell responses through noncanonical autophagy.

The Journal of clinical investigation

Raso F,Sagadiev S,Du S,Gage E,Arkatkar T,Metzler G,Stuart LM,Orr MT,Rawlings DJ,Jackson SW,Lacy-Hulbert A,Acharya M

Published figure using GL7 monoclonal antibody (Product # 14-5902-82) in Flow Cytometry

Fri Aug 31 00:00:00 EDT 2018

Fas ligand promotes an inducible TLR-dependent model of cutaneous lupus-like inflammation.

The Journal of clinical investigation

Mande P,Zirak B,Ko WC,Taravati K,Bride KL,Brodeur TY,Deng A,Dresser K,Jiang Z,Ettinger R,Fitzgerald KA,Rosenblum MD,Harris JE,Marshak-Rothstein A

14-5902 was used in Flow cytometry/Cell sorting to provide a relevant system for exploring the pathophysiology of cutaneous lupus erythematosus (CLE) as well as the negative regulatory role of TLR9.

Mon Jul 02 00:00:00 EDT 2018

Cleavage of TL1A Differentially Regulates Its Effects on Innate and Adaptive Immune Cells.

Journal of immunology (Baltimore, Md. : 1950)

Ferdinand JR,Richard AC,Meylan F,Al-Shamkhani A,Siegel RM

14-5902 was used in Flow cytometry/Cell sorting to suggest differential roles for membrane and soluble TL1A on adaptive and innate immune cells and have implications for the consequences of blocking these two forms of TL1A.

Thu Feb 15 00:00:00 EST 2018

Anti‑IL‑39 (IL‑23p19/Ebi3) polyclonal antibodies ameliorate autoimmune symptoms in lupus‑like mice.

Molecular medicine reports

Wang X,Zhang Y,Wang Z,Liu X,Zhu G,Han G,Chen G,Hou C,Wang T,Shen B,Li Y,Xiao H,Ma N,Wang R

Published figure using GL7 monoclonal antibody (Product # 14-5902-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

B cell activation and plasma cell differentiation are inhibited by de novo DNA methylation.

Nature communications

Barwick BG,Scharer CD,Martinez RJ,Price MJ,Wein AN,Haines RR,Bally APR,Kohlmeier JE,Boss JM

Published figure using GL7 monoclonal antibody (Product # 14-5902-82) in Immunofluorescence

Tue May 15 00:00:00 EDT 2018

Act1 is a negative regulator in T and B cells via direct inhibition of STAT3.

Nature communications

Zhang CJ,Wang C,Jiang M,Gu C,Xiao J,Chen X,Martin BN,Tang F,Yamamoto E,Xian Y,Wang H,Li F,Sartor RB,Smith H,Husni ME,Shi FD,Gao J,Carman J,Dongre A,McKarns SC,Coppieters K,Jørgensen TN,Leonard WJ,Li X

Published figure using GL7 monoclonal antibody (Product # 14-5902-82) in Flow Cytometry

Mon Jul 16 00:00:00 EDT 2018

Peli1 negatively regulates noncanonical NF-κB signaling to restrain systemic lupus erythematosus.

Nature communications

Liu J,Huang X,Hao S,Wang Y,Liu M,Xu J,Zhang X,Yu T,Gan S,Dai D,Luo X,Lu Q,Mao C,Zhang Y,Shen N,Li B,Huang M,Zhu X,Jin J,Cheng X,Sun SC,Xiao Y

Published figure using GL7 monoclonal antibody (Product # 14-5902-82) in Flow Cytometry

Mon Mar 19 00:00:00 EDT 2018

Dissection of SAP-dependent and SAP-independent SLAM family signaling in NKT cell development and humoral immunity.

The Journal of experimental medicine

Chen S,Cai C,Li Z,Liu G,Wang Y,Blonska M,Li D,Du J,Lin X,Yang M,Dong Z

14-5902-82 was used in Flow Cytometry to examine NKT cell development and humoral immunity in mice completely deficient in SFR.

Wed Feb 01 00:00:00 EST 2017

Defective germinal center B-cell response and reduced arthritic pathology in microRNA-29a-deficient mice.

Cellular and molecular life sciences : CMLS

van Nieuwenhuijze A,Dooley J,Humblet-Baron S,Sreenivasan J,Koenders M,Schlenner SM,Linterman M,Liston A

14590282 was used in flow cytometry to evaluate miR-29a in B cells as a potential therapeutic target in arthritis

Thu Jun 01 00:00:00 EDT 2017

Deficiency in Calcium-Binding Protein S100A4 Impairs the Adjuvant Action of Cholera Toxin.

Frontiers in immunology

Sun JB,Holmgren J,Larena M,Terrinoni M,Fang Y,Bresnick AR,Xiang Z

Published figure using GL7 monoclonal antibody (Product # 14-5902-82) in Immunofluorescence

Wed Nov 20 00:00:00 EST 2019

NCR1-deficiency diminishes the generation of protective murine cytomegalovirus antibodies by limiting follicular helper T-cell maturation.

European journal of immunology

Miletic A,Lenartic M,Popovic B,Brizic I,Trsan T,Miklic K,Mandelboim O,Krmpotic A,Jonjic S

14-5902 was used in Flow cytometry/Cell sorting to provide evidence for the role of NCR1 in antibody response to mouse cytomegalovirus infection.

Fri Sep 01 00:00:00 EDT 2017

The Ox40/Ox40 Ligand Pathway Promotes Pathogenic Th Cell Responses, Plasmablast Accumulation, and Lupus Nephritis in NZB/W F1 Mice.

Journal of immunology (Baltimore, Md. : 1950)

Sitrin J,Suto E,Wuster A,Eastham-Anderson J,Kim JM,Austin CD,Lee WP,Behrens TW

14-5902 was used in Immunohistochemistry-immunofluorescence to support the hypothesis that the Ox40/Ox40L pathway drives cellular and humoral autoimmune responses during lupus nephritis in NZB/W F1.

Tue Aug 15 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using GL7 monoclonal antibody (Product # 14-5902-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Tetraspanin CD37 protects against the development of B cell lymphoma.

The Journal of clinical investigation

de Winde CM,Veenbergen S,Young KH,Xu-Monette ZY,Wang XX,Xia Y,Jabbar KJ,van den Brand M,van der Schaaf A,Elfrink S,van Houdt IS,Gijbels MJ,van de Loo FA,Bennink MB,Hebeda KM,Groenen PJ,van Krieken JH,Figdor CG,van Spriel AB

14-5902 was used in Immunohistochemistry to show that CD37 deficiency drives B cell lymphomagenesis through constitutive activation of the IL-6 pathway, highlighting a possible therapeutic intervention.

Mon Feb 01 00:00:00 EST 2016

Deficiency of base excision repair enzyme NEIL3 drives increased predisposition to autoimmunity.

The Journal of clinical investigation

Massaad MJ,Zhou J,Tsuchimoto D,Chou J,Jabara H,Janssen E,Glauzy S,Olson BG,Morbach H,Ohsumi TK,Schmitz K,Kyriacos M,Kane J,Torisu K,Nakabeppu Y,Notarangelo LD,Chouery E,Megarbane A,Kang PB,Al-Idrissi E,Aldhekri H,Meffre E,Mizui M,Tsokos GC,Manis JP,Al-Herz W,Wallace SS,Geha RS

14590282 was used in flow cytometry to identify and characterize a Nei endonuclease VIII-like 3 mutation in three siblings from a consanguineous family with autoimmunity

Tue Nov 01 00:00:00 EDT 2016

Limited clonal relatedness between gut IgA plasma cells and memory B cells after oral immunization.

Nature communications

Bemark M,Hazanov H,Strömberg A,Komban R,Holmqvist J,Köster S,Mattsson J,Sikora P,Mehr R,Lycke NY

Published figure using GL7 monoclonal antibody (Product # 14-5902-82) in Immunofluorescence

Tue Sep 06 00:00:00 EDT 2016

Injectable, spontaneously assembling, inorganic scaffolds modulate immune cells in vivo and increase vaccine efficacy.

Nature biotechnology

Kim J,Li WA,Choi Y,Lewin SA,Verbeke CS,Dranoff G,Mooney DJ

14590282 was used in flow cytometry to investigate the application of intra-dermal mesoporous silica rods in immune modulation.

Thu Jan 01 00:00:00 EST 2015

LEF-1 and TCF-1 orchestrate T(FH) differentiation by regulating differentiation circuits upstream of the transcriptional repressor Bcl6.

Nature immunology

Choi YS,Gullicksrud JA,Xing S,Zeng Z,Shan Q,Li F,Love PE,Peng W,Xue HH,Crotty S

14-5902 was used in Flow cytometry/Cell sorting to report that selective loss of Lef1 or Tcf7 results in T(FH) cell defects.

Tue Sep 01 00:00:00 EDT 2015

Targeting Syk-activated B cells in murine and human chronic graft-versus-host disease.

Blood

Flynn R,Allen JL,Luznik L,MacDonald KP,Paz K,Alexander KA,Vulic A,Du J,Panoskaltsis-Mortari A,Taylor PA,Poe JC,Serody JS,Murphy WJ,Hill GR,Maillard I,Koreth J,Cutler CS,Soiffer RJ,Antin JH,Ritz J,Chao NJ,Clynes RA,Sarantopoulos S,Blazar BR

14-5902 was used in Flow cytometry/Cell sorting to further evaluate the role of the spleen tyrosine kinase in graft-versus-host disease in multiple murine models and human peripheral blood cells.

Thu Jun 25 00:00:00 EDT 2015

IDO1 suppresses inhibitor development in hemophilia A treated with factor VIII.

The Journal of clinical investigation

Matino D,Gargaro M,Santagostino E,Di Minno MN,Castaman G,Morfini M,Rocino A,Mancuso ME,Di Minno G,Coppola A,Talesa VN,Volpi C,Vacca C,Orabona C,Iannitti R,Mazzucconi MG,Santoro C,Tosti A,Chiappalupi S,Sorci G,Tagariello G,Belvini D,Radossi P,Landolfi R,Fuchs D,Boon L,Pirro M,Marchesini E,Grohmann U,Puccetti P,Iorio A,Fallarino F

14-5902 was used in Flow cytometry/Cell sorting to investigate the association between IDO1 competence and inhibitor status in haemophilia A patients.

Thu Oct 01 00:00:00 EDT 2015

Enhanced CD8 T cell responses through GITR-mediated costimulation resolve chronic viral infection.

PLoS pathogens

Pascutti MF,Geerman S,Slot E,van Gisbergen KP,Boon L,Arens R,van Lier RA,Wolkers MC,Nolte MA

14-5902 was used in Flow cytometry/Cell sorting to study resolution of chronic viral infection through GITR-mediated co-stimulation enhancing CD8 T cell responses.

Sun Mar 01 00:00:00 EST 2015

T follicular helper, but not Th1, cell differentiation in the absence of conventional dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Dahlgren MW,Gustafsson-Hedberg T,Livingston M,Cucak H,Alsén S,Yrlid U,Johansson-Lindbom B

14-5902 was used in Flow cytometry/Cell sorting to investigate the accessory role of conventional DCs during the concurrent development of Tfh and effector Th1 cells.

Mon Jun 01 00:00:00 EDT 2015

TNF signals are dispensable for the generation of CD23+ CD21/35-high CD1d-high B cells in inflamed lymph nodes.

Cellular immunology

Kuzin II,Bouta EM,Schwarz EM,Bottaro A

14-5902 was used in Flow cytometry/Cell sorting to investigate the role of tumor necrosis factor signals in producing and maintaining B cells in inflamed lymph nodes, and their role in rheumatoid arthritis pathogenesis.

Sat Aug 01 00:00:00 EDT 2015

BILL-cadherin/cadherin-17 contributes to the survival of memory B cells.

PloS one

Funakoshi S,Shimizu T,Numata O,Ato M,Melchers F,Ohnishi K

Published figure using GL7 monoclonal antibody (Product # 14-5902-82) in Flow Cytometry

Wed Sep 16 00:00:00 EDT 2015

Mutation of POLB causes lupus in mice.

Cell reports

Senejani AG,Liu Y,Kidane D,Maher SE,Zeiss CJ,Park HJ,Kashgarian M,McNiff JM,Zelterman D,Bothwell AL,Sweasy JB

Published figure using GL7 monoclonal antibody (Product # 14-5902-82) in Flow Cytometry

Thu Jan 16 00:00:00 EST 2014

Plasticity of Th17 cells in Peyer's patches is responsible for the induction of T cell-dependent IgA responses.

Nature immunology

Hirota K,Turner JE,Villa M,Duarte JH,Demengeot J,Steinmetz OM,Stockinger B

14-5902 was used in Flow cytometry/Cell sorting to provide evidence that TH17 cells are the crucial subset required for the production of high-affinity T cell-dependent IgA.

Mon Apr 01 00:00:00 EDT 2013

Natural IgG antibodies provide innate protection against ficolin-opsonized bacteria.

The EMBO journal

Panda S,Zhang J,Tan NS,Ho B,Ding JL

Published figure using GL7 monoclonal antibody (Product # 14-5902-82) in Immunofluorescence

Wed Nov 13 00:00:00 EST 2013

SAP modulates B cell functions in a genetic background-dependent manner.

Immunology letters

Detre C,Yigit B,Keszei M,Castro W,Magelky EM,Terhorst C

14-5902 was used in Flow cytometry/Cell sorting to investigate the mechanism by which SLAM-associated protein modulates B cell functions.

Sat Jun 01 00:00:00 EDT 2013

Peroxisome proliferator-activated receptor γ B cell-specific-deficient mice have an impaired antibody response.

Journal of immunology (Baltimore, Md. : 1950)

Ramon S,Bancos S,Thatcher TH,Murant TI,Moshkani S,Sahler JM,Bottaro A,Sime PJ,Phipps RP

14-5902 was used in Flow cytometry/Cell sorting to study how PPARγ expression in B cells is required for an efficient B cell-mediated immune response by regulating B cell differentiation and Ab production.

Thu Nov 15 00:00:00 EST 2012

The viral latency-associated nuclear antigen augments the B-cell response to antigen in vivo.

Journal of virology

Sin SH,Fakhari FD,Dittmer DP

14-5902 was used in Immunohistochemistry to examine whether the Kaposi sarcoma-associated herpesvirus latency-associated nuclear antigen provides a selective advantage to infected B cells by driving proliferation in response to antigen.

Fri Oct 01 00:00:00 EDT 2010

Human B-lymphocytes express alpha2-6-sialylated 6-sulfo-N-acetyllactosamine serving as a preferred ligand for CD22/Siglec-2.

The Journal of biological chemistry

Kimura N,Ohmori K,Miyazaki K,Izawa M,Matsuzaki Y,Yasuda Y,Takematsu H,Kozutsumi Y,Moriyama A,Kannagi R

Fri Nov 02 00:00:00 EDT 2007

TLR4 on follicular dendritic cells: an activation pathway that promotes accessory activity.

Journal of immunology (Baltimore, Md. : 1950)

El Shikh ME,El Sayed RM,Wu Y,Szakal AK,Tew JG

14-5902-82 was used in Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation to report expression of TLR4 on FDCs for the first time and that engagement of FDC-TLR4 activated NF-kappaB, up-regulated expression of molecules important in FDC accessory function, including Fc gammaRIIB, ICAM-1, and VCAM-1, as well as FDC accessory activity in promoting recall IgG responses.

Mon Oct 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: GL7 antigen; Ly-77; Ly77

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GL7 Monoclonal Antibody (GL-7 (GL7)), eBioscience™

GL7 Antibody (14-5902-82) in Flow

Product Details

14-5902-82

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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