Invitrogen

MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), eBioscience™

MHC Class I (H-2Db) Antibody in Flow Cytometry (Flow)

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MHC Class I (H-2Db) Antibody (14-5999-82) in Flow

Oncogene 2019 - Figure 6. CXCL14-mediated tumor suppression is abrogated by MHC-I knockdown in HNC cells. MOE/E6E7 CXCL14 cells were transduced with shRNAs directed to B2M and enriched for knockdown by FACS sorting for the bottom 10% of MHC-I expressing cells (MOE/E6E7 CXCL14/shB2M ). Flow cytometric analysis of H-2D b (A) and H-2K b (B) MHC-I molecules in MOE/E6E7 Vector (gray), MOE/E6E7 CXCL14 (blue), and MOE/E6E7 ... View More

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Product Details

14-5999-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 7 publications 7 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

28-14-8

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467825

Product Specific Information

Description: The 28-14-8 monoclonal antibody reacts with the mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q and/or H-2L^q. Binding of 28-14-8 to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin.

Applications Reported: 28-14-8 has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen mouse tissue sections, immunoprecipitation and complement-dependent cytotoxicity.

Applications Tested: The 28-14-8 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CXCL14 suppresses human papillomavirus-associated head and neck cancer through antigen-specific CD8⁺ T-cell responses by upregulating MHC-I expression.

Oncogene

Westrich JA,Vermeer DW,Silva A,Bonney S,Berger JN,Cicchini L,Greer RO,Song JI,Raben D,Slansky JE,Lee JH,Spanos WC,Pyeon D

Published figure using MHC Class I (H-2Db) monoclonal antibody (Product # 14-5999-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2019

Macrophage-specific MHCII expression is regulated by a remote Ciita enhancer controlled by NFAT5.

The Journal of experimental medicine

Buxadé M,Huerga Encabo H,Riera-Borrull M,Quintana-Gallardo L,López-Cotarelo P,Tellechea M,Martínez-Martínez S,Redondo JM,Martín-Caballero J,Flores JM,Bosch E,Rodríguez-Fernández JL,Aramburu J,López-Rodríguez C

Published figure using MHC Class I (H-2Db) monoclonal antibody (Product # 14-5999-82) in Flow Cytometry

Mon Nov 05 00:00:00 EST 2018

The Shc1 adaptor simultaneously balances Stat1 and Stat3 activity to promote breast cancer immune suppression.

Nature communications

Ahn R,Sabourin V,Bolt AM,Hébert S,Totten S,De Jay N,Festa MC,Young YK,Im YK,Pawson T,Koromilas AE,Muller WJ,Mann KK,Kleinman CL,Ursini-Siegel J

Published figure using MHC Class I (H-2Db) monoclonal antibody (Product # 14-5999-82) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2017

Preclinical Development of Ipilimumab and Nivolumab Combination Immunotherapy: Mouse Tumor Models, In Vitro Functional Studies, and Cynomolgus Macaque Toxicology.

PloS one

Selby MJ,Engelhardt JJ,Johnston RJ,Lu LS,Han M,Thudium K,Yao D,Quigley M,Valle J,Wang C,Chen B,Cardarelli PM,Blanset D,Korman AJ

14-5999 was used in Flow cytometry/Cell sorting to exhibit a preclinical strategy for the identification and development of highly effective antitumour combination immunotherapies.

Fri Aug 25 00:00:00 EDT 2017

Ly6C(high) monocytes control cerebral toxoplasmosis.

Journal of immunology (Baltimore, Md. : 1950)

Biswas A,Bruder D,Wolf SA,Jeron A,Mack M,Heimesaat MM,Dunay IR

14-5999 was used in Flow cytometry/Cell sorting to indicate the critical importance of recruited Ly6C monocytes upon cerebral toxoplasmosis.

Wed Apr 01 00:00:00 EDT 2015

Enhanced cross-presentation and improved CD8+ T cell responses after mannosylation of synthetic long peptides in mice.

PloS one

Rauen J,Kreer C,Paillard A,van Duikeren S,Benckhuijsen WE,Camps MG,Valentijn AR,Ossendorp F,Drijfhout JW,Arens R,Burgdorf S

14-5999 was used in Flow cytometry/Cell sorting to demonstrate that mannosylation of synthetic long peptides augments the induction of a cytotoxic T-cell response.

Mon Oct 26 00:00:00 EDT 2015

A LysM and SH3-domain containing region of the Listeria monocytogenes p60 protein stimulates accessory cells to promote activation of host NK cells.

PLoS pathogens

Schmidt RL,Filak HC,Lemon JD,Potter TA,Lenz LL

14-5999 was used in Flow cytometry/Cell sorting to demonstrate an immune stimulating function for a bacterial LysM domain-containing polypeptide.

Tue Nov 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: H-2 class I histocompatibility antigen, D-B alpha chain; H-2D cell surface glycoprotein; H-2D(B); H2-DC1-beta transplantation antigen protein; H2D1; H2DB; H2L; H2LD; MHC class I antigen; MHC class I H-2Dp; MHC class I H2 antigen; MHC H2-D-q alpha-chain; Q5k protein

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Gene Aliases: H-2D; H2-D; H2-D1

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UniProt ID: (Mouse) P01899

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Entrez Gene ID: (Mouse) 14964

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Function(s)

receptor binding protein binding beta-2-microglobulin binding peptide binding peptide antigen binding T cell receptor binding poly(A) RNA binding TAP binding protein heterodimerization activity major histocompatibility complex protein

Process(es)

positive regulation of T cell mediated cytotoxicity immune system process antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent immune response antigen processing and presentation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), eBioscience™

MHC Class I (H-2Db) Antibody (14-5999-82) in Flow

Product Details

14-5999-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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