Invitrogen

c-Rel Monoclonal Antibody (1RELAH5), eBioscience™

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

4 Published Figures

7 References

View all (26) c-Rel antibodies

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Datasheet

Protocols

Questions & Answers

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c-Rel Antibody (14-6111-82) in WB

Western blot was performed using anti-c-REL Monoclonal Antibody(Product # 14-6111-82) and 65 kDa band was observed corresponding to c-REL across the Tissues tested. Tissue Extracts of (30 µg lysate) of Mouse Thymus (Lane 1),Mouse Spleen (Lane 2), Mouse Lung (Lane 3), Mouse Small Intestine (Lane 4) and Mouse Heart (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by XCell SureLock™ Mini-Cell and XCell II™ Bl... View More

c-Rel Antibody in Immunocytochemistry (ICC/IF)

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c-Rel Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of c-REL was observed in Mouse Thymus, Mouse spleen and Mouse Lung in comparison to no expression in Mouse Heart and Mouse Small Intestine using anti-c-REL Monoclonal Antibody(Product # 14-6111-82) in Western Blot. {RE}

Product Details

14-6111-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

1RELAH5

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11043473

Product Specific Information

Description: The 1RELAH5 monoclonal antibody reacts with mouse c-Rel. c-Rel is a 65 kDa hematopoietic lineage-restricted member of the NF-kB family of transcription factors. In the thymus, c-Rel is upregulated in regulatory T cells (Treg) when compared to other CD4+ thymocytes. c-Rel is believed to play a critical role in thymic Treg (nTreg) development, but is not necessary for TGF beta-mediated T reg (iTreg) differentiation. C-Rel is also involved in proliferation and survival in mature T cells.

Applications Reported: This 1RELAH5 antibody has been reported for use in western blotting.

Applications Tested: This 1RELAH5 antibody has been tested by western blot analysis of mouse splenocytes. This can be used at 1-5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA., RELB (604758), NFKB1, and NFKB2. These proteins are related through a highly conserved N-terminal region termed the 'Rel domain, ' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (Belguise and Sonenshein, 2007).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Toll-like receptor mediated inflammation directs B cells towards protective antiviral extrafollicular responses.

Nature communications

Lam JH,Baumgarth N

Published figure using c-Rel monoclonal antibody (Product # 14-6111-82) in Flow Cytometry

Wed Jul 05 00:00:00 EDT 2023

Differential cell-intrinsic regulations of germinal center B and T cells by miR-146a and miR-146b.

Nature communications

Cho S,Lee HM,Yu IS,Choi YS,Huang HY,Hashemifar SS,Lin LL,Chen MC,Afanasiev ND,Khan AA,Lin SW,Rudensky AY,Crotty S,Lu LF

Published figure using c-Rel monoclonal antibody (Product # 14-6111-82) in Western Blot

Mon Jul 16 00:00:00 EDT 2018

Roquin targets mRNAs in a 3'-UTR-specific manner by different modes of regulation.

Nature communications

Essig K,Kronbeck N,Guimaraes JC,Lohs C,Schlundt A,Hoffmann A,Behrens G,Brenner S,Kowalska J,Lopez-Rodriguez C,Jemielity J,Holtmann H,Reiche K,Hackermüller J,Sattler M,Zavolan M,Heissmeyer V

14-6111 was used in Western Blotting to study how the robustness and magnitude of Roquin-mediated regulation is encoded in complex cis-elements.

Wed Sep 19 00:00:00 EDT 2018

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

14-6111 was used in Western Blotting to show that miR-27 has a role in regulating Treg immune cells and that correct control of miR-27 expression is important to safeguard immunological tolerance..

Wed Feb 01 00:00:00 EST 2017

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

14-6111 was used in Western Blotting to show that miR-27 has a role in regulating Treg immune cells and that correct control of miR-27 expression is important to safeguard immunological tolerance..

Wed Feb 01 00:00:00 EST 2017

A multi-scale approach reveals that NF-κB cRel enforces a B-cell decision to divide.

Molecular systems biology

Shokhirev MN,Almaden J,Davis-Turak J,Birnbaum HA,Russell TM,Vargas JA,Hoffmann A

Published figure using c-Rel monoclonal antibody (Product # 14-6111-82) in Flow Cytometry

Fri Feb 13 00:00:00 EST 2015

Cutting Edge: Synchronization of IRF1, JunB, and C/EBPβ Activities during TLR3-TLR7 Cross-Talk Orchestrates Timely Cytokine Synergy in the Proinflammatory Response.

Journal of immunology (Baltimore, Md. : 1950)

Liu Q,Zhu Y,Yong WK,Sze NS,Tan NS,Ding JL

14-6111 was used in Western Blotting to investigate the detailed mechanisms of multiple pathogen-associated molecular pattern-induced TLR pathway cross-talk.

Sat Aug 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: BCD541; C-Rel protein; C-Rel proto-oncogene protein; gemin 1; gemin-1; proto-oncogene c-Rel; SMA; SMA1; SMA2; SMA3; SMA4; SMN; SMN2; SMNT

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Gene Aliases: c-Rel

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Entrez Gene ID: (Mouse) 19696

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding sequence-specific DNA binding Rel homology transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter cytokine production transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter inflammatory response I-kappaB kinase/NF-kappaB signaling negative regulation of gene expression negative regulation of interferon-beta production response to cytokine NIK/NF-kappaB signaling positive regulation of interleukin-12 biosynthetic process innate immune response positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of neuron death

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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c-Rel Monoclonal Antibody (1RELAH5), eBioscience™

c-Rel Antibody (14-6111-82) in WB

Product Details

14-6111-82

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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