Invitrogen

CD261 (DR4) Monoclonal Antibody (DJR1), eBioscience™

6 Published Figures

9 References

View all (31) DR4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (6)

CD261 (DR4) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 7

CD261 (DR4) Antibody (14-6644-82) in Flow

Staining of human DR4-transfected cells withMouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (open histogram) or Anti-Human CD261 (DR4) Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Total viable cells were used for analysis.

CD261 (DR4) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD261 (DR4) Antibody in Western Blot (WB)

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Product Details

14-6644-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

DJR1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468188

Product Specific Information

Description: The DJR1 monoclonal antibody reacts with human DR4, also known as TRAIL-R1. DR4 binds to TRAIL, activates NF-kappaB, and induces TRAIL-mediated apoptosis. DR4 is expressed by a variety of human tumor cells and at very low level on human leukocytes.

Expression has also been found inside the cell on some cell lines (Zhang et al). This has been confirmed with intracellular staining of Jurkat cell line. Please contact tech@ebioscience.com for more information.

Applications Reported: This DJR1 antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections.

Applications Tested: The DJR1 antibody has been tested by flow cytometric analysis of human DR4-transfected cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TRAIL-R1 (CD261, DR4) is a type I transmembrane protein, also called TRAIL receptor 1. The ligand for this DR4 death receptor has been identified and termed TRAIL, which is a member of the TNF family. DR4, as many other receptors (Fas, TNFR1, etc.), mediates apoptosis and NF kappaB activation in many cells and tissues. Apoptosis, a programmed cell death, is a operating process in normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by coupled of certain cytokines (TNF family - TNF, Fas ligand) and their death domain containing receptors (TNFR1, Fas receptor).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Systematic genetic mapping of necroptosis identifies SLC39A7 as modulator of death receptor trafficking.

Cell death and differentiation

Fauster A,Rebsamen M,Willmann KL,César-Razquin A,Girardi E,Bigenzahn JW,Schischlik F,Scorzoni S,Bruckner M,Konecka J,Hörmann K,Heinz LX,Boztug K,Superti-Furga G

Published figure using CD261 (DR4) monoclonal antibody (Product # 14-6644-82) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2019

Targeting the metabolic pathway of human colon cancer overcomes resistance to TRAIL-induced apoptosis.

Cell death discovery

Carr RM,Qiao G,Qin J,Jayaraman S,Prabhakar BS,Maker AV

Published figure using CD261 (DR4) monoclonal antibody (Product # 14-6644-82) in Western Blot

Tue Sep 20 00:00:00 EDT 2016

TRAIL-mediated apoptosis in breast cancer cells cultured as 3D spheroids.

PloS one

Chandrasekaran S,Marshall JR,Messing JA,Hsu JW,King MR

Published figure using CD261 (DR4) monoclonal antibody (Product # 14-6644-82) in Immunofluorescence

Thu Dec 24 00:00:00 EST 2015

TRAIL-mediated apoptosis in breast cancer cells cultured as 3D spheroids.

PloS one

Chandrasekaran S,Marshall JR,Messing JA,Hsu JW,King MR

Published figure using CD261 (DR4) monoclonal antibody (Product # 14-6644-82) in Immunofluorescence

Thu Dec 24 00:00:00 EST 2015

A whole-genome RNAi screen identifies an 8q22 gene cluster that inhibits death receptor-mediated apoptosis.

Proceedings of the National Academy of Sciences of the United States of America

Dompe N,Rivers CS,Li L,Cordes S,Schwickart M,Punnoose EA,Amler L,Seshagiri S,Tang J,Modrusan Z,Davis DP

Published figure using CD261 (DR4) monoclonal antibody (Product # 14-6644-82) in Flow Cytometry

Tue Oct 25 00:00:00 EDT 2011

ABT-737 induces expression of the death receptor 5 and sensitizes human cancer cells to TRAIL-induced apoptosis.

The Journal of biological chemistry

Song JH,Kandasamy K,Kraft AS

14-6644 was used in Flow cytometry/Cell sorting to demonstrate that a combination of ABT-737 and TRAIL induced significant cell death in multiple cancer types.

Fri Sep 05 00:00:00 EDT 2008

The proteasome inhibitor PS-341 (bortezomib) up-regulates DR5 expression leading to induction of apoptosis and enhancement of TRAIL-induced apoptosis despite up-regulation of c-FLIP and survivin expression in human NSCLC cells.

Cancer research

Liu X,Yue P,Chen S,Hu L,Lonial S,Khuri FR,Sun SY

14-6644 was used in Flow cytometry/Cell sorting to show the important role of DR5 up-regulation in human NSCLC cells in PS-341 and TRAIL induced apoptosis.

Tue May 15 00:00:00 EDT 2007

The farnesyltransferase inhibitor lonafarnib induces CCAAT/enhancer-binding protein homologous protein-dependent expression of death receptor 5, leading to induction of apoptosis in human cancer cells.

The Journal of biological chemistry

Sun SY,Liu X,Zou W,Yue P,Marcus AI,Khuri FR

14-6644 was used in Flow cytometry/Cell sorting to investigate the underlying mechanism by which farnesyltransferase inhibitors cause growth arrest or apoptosis in cancer cells, showing that it induces expression of death receptor 5.

Fri Jun 29 00:00:00 EDT 2007

Difference in cytotoxicity against hepatocellular carcinoma between liver and periphery natural killer cells in humans.

Hepatology (Baltimore, Md.)

Ishiyama K,Ohdan H,Ohira M,Mitsuta H,Arihiro K,Asahara T

Wed Feb 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: CD261; cytotoxic TRAIL receptor; Death receptor 4; MGC9365; sCD261; soluble CD261; soluble TRAIL; sTRAIL; TNF-related apoptosis-inducing ligand receptor 1; TRAIL R1; TRAIL receptor 1; TRAIL-R1; Tumor necrosis factor receptor superfamily member 10A; tumor necrosis factor receptor superfamily member 10a variant 2; tumor necrosis factor receptor superfamily, member 10a

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Gene Aliases: APO2; CD261; DR4; TNFRSF10A; TRAILR-1; TRAILR1

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UniProt ID: (Human) O00220

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Entrez Gene ID: (Human) 8797

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Function(s)

protease binding receptor activity tumor necrosis factor-activated receptor activity death receptor activity protein binding transcription factor binding TRAIL binding transmembrane signal receptor

Process(es)

apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process inflammatory response immune response signal transduction cell surface receptor signaling pathway activation of NF-kappaB-inducing kinase activity multicellular organism development extrinsic apoptotic signaling pathway via death domain receptors response to lipopolysaccharide tumor necrosis factor-mediated signaling pathway TRAIL-activated apoptotic signaling pathway regulation of cell proliferation regulation of apoptotic process cellular response to mechanical stimulus extrinsic apoptotic signaling pathway activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of extrinsic apoptotic signaling pathway via death domain receptors

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD261 (DR4) Monoclonal Antibody (DJR1), eBioscience™

CD261 (DR4) Antibody (14-6644-82) in Flow

Product Details

14-6644-82

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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