Invitrogen

IL-4 Monoclonal Antibody (MP4-25D2), eBioscience™

3 Published Figures

13 References

View all (109) IL-4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (3)

FIGURE: 1 / 3

IL-4 Antibody (14-7048-81) in Flow

Scientific reports 2023 - Inhibition of DYRK1B function suppresses Th1 and Th17, but enhances Treg differentiation. ( A-C ) Human naive CD4 T cells were stimulated by anti-CD3 and anti-CD28 and then differentiated under Th1-, Th17- and Th2-polarizing conditions, respectively, in the absence or presence of a selective DYRK1B inhibitor at 4 different concentrations for 96 h. The scatter dot plots represent data from triplicate sample... View More

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Product Details

14-7048-81

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 7 publications 7 publications

ELISA (ELISA)

View 1 publication 1 publication

Neutralization (Neu)

View 2 publications 2 publications

Functional Assay (Functional)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

MP4-25D2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468414

Product Specific Information

Description: The MP4-25D2 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.

Applications Reported: MP4-25D2 has been reported for use in neutralizing IL-4 binding and biological activity. (Please use Functional Grade purified MP4-25D2, Product # 16-7048 , in functional assays.) Please refer to the supporting protocols for more information.

Applications Tested: The purified MP4-25D2 can be used for blocking of fluorochrome conjugated MP4-25D2 in intracellular flow cytometry experiments to serve as specificity control. This biotinylated format for this lot of MP4-25D2 has been tested by sandwich ELISA for detection of human IL-4 in combination with 8D4-8 and can be used at 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of DYRK1B suppresses inflammation in allergic contact dermatitis model and Th1/Th17 immune response.

Scientific reports

Wongchang T,Pluangnooch P,Hongeng S,Wongkajornsilp A,Thumkeo D,Soontrapa K

Published figure using IL-4 monoclonal antibody (Product # 14-7048-81) in Flow Cytometry

Sat Apr 29 00:00:00 EDT 2023

Fasting-induced FOXO4 blunts human CD4⁺ T helper cell responsiveness.

Nature metabolism

Han K,Singh K,Rodman MJ,Hassanzadeh S,Wu K,Nguyen A,Huffstutler RD,Seifuddin F,Dagur PK,Saxena A,McCoy JP,Chen J,Biancotto A,Stagliano KER,Teague HL,Mehta NN,Pirooznia M,Sack MN

Published figure using IL-4 monoclonal antibody (Product # 14-7048-81) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

Reciprocal regulation of Th2 and Th17 cells by PAD2-mediated citrullination.

JCI insight

Sun B,Chang HH,Salinger A,Tomita B,Bawadekar M,Holmes CL,Shelef MA,Weerapana E,Thompson PR,Ho IC

Published figure using IL-4 monoclonal antibody (Product # 14-7048-81) in ELISA

Thu Nov 14 00:00:00 EST 2019

IL-6/STAT3 pathway induced deficiency of RFX1 contributes to Th17-dependent autoimmune diseases via epigenetic regulation.

Nature communications

Zhao M,Tan Y,Peng Q,Huang C,Guo Y,Liang G,Zhu B,Huang Y,Liu A,Wang Z,Li M,Gao X,Wu R,Wu H,Long H,Lu Q

14-7048 was used in Functional assays to show reduced expression of the transcription factor RFX1 in CD4+ T cells from patients with systemic lupus erythematosus which leads to IL-17A overexpression through increased histone H3 acetylation and decreased DNA methylation.

Thu Feb 08 00:00:00 EST 2018

An asthma-associated IL4R variant exacerbates airway inflammation by promoting conversion of regulatory T cells to TH17-like cells.

Nature medicine

Massoud AH,Charbonnier LM,Lopez D,Pellegrini M,Phipatanakul W,Chatila TA

14-7048 was used in Flow cytometry/Cell sorting to identify a previously unknown mechanism for the development of TH17 cell inflammation in genetically prone individuals.

Thu Sep 01 00:00:00 EDT 2016

IFN-γ-rich environment programs dendritic cells toward silencing of cytotoxic immune responses.

Journal of leukocyte biology

Svajger U,Obermajer N,Jeras M

14704881 was used in flow cytometry to assess how different concentrations of IFN-gamma affect dendritic cells

Wed Jan 01 00:00:00 EST 2014

Cytokine diversity in the Th1-dominated human anti-influenza response caused by variable cytokine expression by Th1 cells, and a minor population of uncommitted IL-2+IFNγ- Thpp cells.

PloS one

Deng N,Weaver JM,Mosmann TR

14-7048 was used in Flow cytometry/Cell sorting to study expression of IL-2 in T cell subsets during the anti-influenza response.

Thu Dec 18 00:00:00 EST 2014

C3a modulates IL-1β secretion in human monocytes by regulating ATP efflux and subsequent NLRP3 inflammasome activation.

Blood

Asgari E,Le Friec G,Yamamoto H,Perucha E,Sacks SS,Köhl J,Cook HT,Kemper C

14-7048 was used in Neutralization experiments to investigate how TLR signals and ATP availability are regulated during monocyte activation.

Thu Nov 14 00:00:00 EST 2013

Human CD1c⁺ myeloid dendritic cells acquire a high level of retinoic acid-producing capacity in response to vitamin D₃.

Journal of immunology (Baltimore, Md. : 1950)

Sato T,Kitawaki T,Fujita H,Iwata M,Iyoda T,Inaba K,Ohteki T,Hasegawa S,Kawada K,Sakai Y,Ikeuchi H,Nakase H,Niwa A,Takaori-Kondo A,Kadowaki N

14-7048 was used in an ELISA assay to suggest that the "vitamin D-CD1c&8314mDC-RA" axis may constitute an important immune component for maintaining tissue homeostasis in humans.

Sun Sep 15 00:00:00 EDT 2013

Intercellular adhesion molecule 1 mediates migration of Th1 and Th17 cells across human retinal vascular endothelium.

Investigative ophthalmology & visual science

Bharadwaj AS,Schewitz-Bowers LP,Wei L,Lee RW,Smith JR

14-7048 was used in Flow cytometry/Cell sorting to investigate the ability of human Th1- and Th17-polarised cells to cross a simulated human retinal endothelium, and examined the role of IgG superfamily members in this process.

Wed Oct 23 00:00:00 EDT 2013

Dominant Th2 differentiation of human regulatory T cells upon loss of FOXP3 expression.

Journal of immunology (Baltimore, Md. : 1950)

Hansmann L,Schmidl C,Kett J,Steger L,Andreesen R,Hoffmann P,Rehli M,Edinger M

14-7048 was used in Blocking experiments to compare the transcriptomes of stable FOXP3(+) Treg and converted FOXP3(-) ex-Treg cells using a newly developed intranuclear staining protocol.

Wed Feb 01 00:00:00 EST 2012

CD137 ligand signaling induces human monocyte to dendritic cell differentiation.

European journal of immunology

Kwajah M M S,Schwarz H

14-7048 was used in Flow cytometry to deduce that peripheral monocytes can be differentiated into mature inflammatory dendritic cells by the single factor CD137.

Thu Jul 01 00:00:00 EDT 2010

De novo generation and enhanced suppression of human CD4+CD25+ regulatory T cells by retinoic acid.

Journal of immunology (Baltimore, Md. : 1950)

Wang J,Huizinga TW,Toes RE

14-7048 was used in Neutralization experiments to investigate methods to convert T cells into Treg cells, showing that retinoic acid can be used for de novo generation of Tregs.

Tue Sep 15 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: B cell growth factor 1; B-cell stimulatory factor 1; B_cell stimulatory factor 1; Binetrakin; BSF-1; H-IL-4; IL-4; ILN; Interleukin; interleukin 4 variant 2; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402; Pitrakinra

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Gene Aliases: BCGF-1; BCGF1; BSF-1; BSF1; IL-4; IL4

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UniProt ID: (Human) P05112

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Entrez Gene ID: (Human) 3565

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Function(s)

cytokine activity interleukin-4 receptor binding protein binding growth factor activity interleukin superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-4 Monoclonal Antibody (MP4-25D2), eBioscience™

IL-4 Antibody (14-7048-81) in Flow

Product Details

14-7048-81

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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