Invitrogen

IL-4 Monoclonal Antibody (8D4-8), eBioscience™

15 Published Figures

21 References

View all (109) IL-4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (15)

IL-4 Antibody in Western Blot, Flow Cytometry (WB, Flow)

FIGURE: 1 / 15

IL-4 Antibody (14-7049-81) in WB, Flow

Nucleic acids research 2015 - Figure 6. piR30840 regulates the development of Th2 lymphocytes. ( A and B ) Human CD4 naive cells were cultured in a conditioned medium for Th2 development and then transfected with an s30840 (A) or piR30840 inhibitor (B). IL-4 expression was detected by qRT-PCR and western blotting analyses. P < 0.01. The data represents three independent experiments. ( C ) Detection of IL-4 expression in human PBMCs isol... View More

IL-4 Antibody in Radioimmune assays (RIA)

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Product Details

14-7049-81

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 15 publications 15 publications

ELISA (ELISA)

1-4 µg/mL

View 2 publications 2 publications

Radioimmune Assays (RIA)

View 1 publication 1 publication

T-Cell Activation (TCA)

View 1 publication 1 publication

Affinity Purification (AP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Cynomolgus monkey, Human, Mouse, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

8D4-8

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468416

Product Specific Information

Description: The 8D4-8 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.

Applications Reported:The 8D4-8 antibody has been reported for use in capture of human IL-4 by ELISA and in intracellular staining for flow cytometric analysis.

Applications Tested: The 8D4-8 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interleukin-4 (IL-4) in combination with the biotinylated MP4-25D2 (Product # 13-7048-81) antibody for detection and recombinant human IL-4 (Product # 14-8049-80) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate.

The fluorochrome-conjugated 8D4-8 antibody has been tested by intracellular staining for flow cytometric analysis. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation:Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Indole-3-pyruvic acid alleviates rheumatoid arthritis via the aryl hydrocarbon receptor pathway.

Annals of translational medicine

Huang T,Cheng L,Jiang Y,Zhang L,Qian L

14-7049-81 was used in Cell Culture to reveal the effect and mechanism of indole-3-pyruvic acid (IPA) in rheumatoid arthritis (RA).

Wed Mar 15 00:00:00 EDT 2023

Dysregulated Peripheral Invariant Natural Killer T Cells in Plaque Psoriasis Patients.

Frontiers in cell and developmental biology

Hu Y,Chen Y,Chen Z,Zhang X,Guo C,Yu Z,Xu P,Sun L,Zhou X,Gong Y,Yu Q,Shi Y

Published figure using IL-4 monoclonal antibody (Product # 14-7049-81) in Flow Cytometry

Wed Feb 23 00:00:00 EST 2022

Cord Blood T Cells Expressing High and Low PKCζ Levels Develop into Cells with a Propensity to Display Th1 and Th9 Cytokine Profiles, Respectively.

International journal of molecular sciences

Perveen K,Quach A,McPhee A,Prescott SL,Barry SC,Hii CS,Ferrante A

Published figure using IL-4 monoclonal antibody (Product # 14-7049-81) in Flow Cytometry

Wed May 05 00:00:00 EDT 2021

Serum Amyloid A Proteins Induce Pathogenic Th17 Cells and Promote Inflammatory Disease.

Cell

Lee JY,Hall JA,Kroehling L,Wu L,Najar T,Nguyen HH,Lin WY,Yeung ST,Silva HM,Li D,Hine A,Loke P,Hudesman D,Martin JC,Kenigsberg E,Merad M,Khanna KM,Littman DR

14-7049-85 was used in T-Cell Activation to suggest that serum amyloid A proteins direct a pathogenic pro-inflammatory Th17 cell differentiation program and promote Th17-mediated inflammatory diseases.

Thu Jan 09 00:00:00 EST 2020

Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.

Frontiers in immunology

Zu J,Yao L,Song Y,Cui Y,Guan M,Chen R,Zhen Y,Li S

Published figure using IL-4 monoclonal antibody (Product # 14-7049-81) in Flow Cytometry

Mon May 31 00:00:00 EDT 2021

Platelet-Derived GARP Induces Peripheral Regulatory T Cells-Potential Impact on T Cell Suppression in Patients with Melanoma-Associated Thrombocytosis.

Cancers

Zimmer N,Krebs FK,Zimmer S,Mitzel-Rink H,Kumm EJ,Jurk K,Grabbe S,Loquai C,Tuettenberg A

Published figure using IL-4 monoclonal antibody (Product # 14-7049-81) in Flow Cytometry

Sat Dec 05 00:00:00 EST 2020

Standardized 11-color flow cytometry panel for the functional phenotyping of human T regulatory cells.

Journal of biological methods

Manuszak C,Brainard M,Thrash E,Hodi FS,Severgnini M

Published figure using IL-4 monoclonal antibody (Product # 14-7049-81) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Neutrophils express pro- and anti-inflammatory cytokines in granulomas from Mycobacterium tuberculosis-infected cynomolgus macaques.

Mucosal immunology

Gideon HP,Phuah J,Junecko BA,Mattila JT

14-7049-81 was used in Flow Cytometry to compare neutrophil and T-cell expression of the Th1 cytokines IFNγ and TNF, the Th2 cytokine IL-4, and regulatory cytokine IL-10 in M. tuberculosis-infected macaques.

Fri Nov 01 00:00:00 EDT 2019

Neutrophils express pro- and anti-inflammatory cytokines in granulomas from Mycobacterium tuberculosis-infected cynomolgus macaques.

Mucosal immunology

Gideon HP,Phuah J,Junecko BA,Mattila JT

Fri Nov 01 00:00:00 EDT 2019

CD4 T cell loss and Th2 and Th17 bias are associated with the severity of severe fever with thrombocytopenia syndrome (SFTS).

Clinical immunology (Orlando, Fla.)

Li MM,Zhang WJ,Weng XF,Li MY,Liu J,Xiong Y,Xiong SE,Zou CC,Wang H,Lu MJ,Yang DL,Peng C,Zheng X

Published figure using IL-4 monoclonal antibody (Product # 14-7049-81) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2018

Impact of Enhancer of Zeste Homolog 2 on T Helper Cell-Mediated Allergic Rhinitis.

Frontiers in immunology

Hou TY,Chen MR,Chou YC,Kan PC,Tsai YT,Cha TL

Published figure using IL-4 monoclonal antibody (Product # 14-7049-81) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

A molecular signature of preclinical rheumatoid arthritis triggered by dysregulated PTPN22.

JCI insight

Chang HH,Liu GY,Dwivedi N,Sun B,Okamoto Y,Kinslow JD,Deane KD,Demoruelle MK,Norris JM,Thompson PR,Sparks JA,Rao DA,Karlson EW,Hung HC,Holers VM,Ho IC

14-7049 was used in an ELISA assay to depict a molecular signature of preclinical rheumatoid arthritis that is triggered by impaired induction of PTPN22.

Thu Oct 20 00:00:00 EDT 2016

A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes.

Nucleic acids research

Zhong F,Zhou N,Wu K,Guo Y,Tan W,Zhang H,Zhang X,Geng G,Pan T,Luo H,Zhang Y,Xu Z,Liu J,Liu B,Gao W,Liu C,Ren L,Li J,Zhou J,Zhang H

Published figure using IL-4 monoclonal antibody (Product # 14-7049-81) in Flow Cytometry

Wed Dec 02 00:00:00 EST 2015

A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes.

Nucleic acids research

Zhong F,Zhou N,Wu K,Guo Y,Tan W,Zhang H,Zhang X,Geng G,Pan T,Luo H,Zhang Y,Xu Z,Liu J,Liu B,Gao W,Liu C,Ren L,Li J,Zhou J,Zhang H

Published figure using IL-4 monoclonal antibody (Product # 14-7049-81) in Flow Cytometry

Wed Dec 02 00:00:00 EST 2015

Brief Report: IFIH1 Mutation Causes Systemic Lupus Erythematosus With Selective IgA Deficiency.

Arthritis & rheumatology (Hoboken, N.J.)

Van Eyck L,De Somer L,Pombal D,Bornschein S,Frans G,Humblet-Baron S,Moens L,de Zegher F,Bossuyt X,Wouters C,Liston A

14-7049-81 was used in Flow Cytometry to determine the underlying genetic defect responsible for severe early onset and refractory systemic lupus erythematosus.

Mon Jun 01 00:00:00 EDT 2015

Autosomal dominant immune dysregulation syndrome in humans with CTLA4 mutations.

Nature medicine

Schubert D,Bode C,Kenefeck R,Hou TZ,Wing JB,Kennedy A,Bulashevska A,Petersen BS,Schäffer AA,Grüning BA,Unger S,Frede N,Baumann U,Witte T,Schmidt RE,Dueckers G,Niehues T,Seneviratne S,Kanariou M,Speckmann C,Ehl S,Rensing-Ehl A,Warnatz K,Rakhmanov M,Thimme R,Hasselblatt P,Emmerich F,Cathomen T,Backofen R,Fisch P,Seidl M,May A,Schmitt-Graeff A,Ikemizu S,Salzer U,Franke A,Sakaguchi S,Walker LSK,Sansom DM,Grimbacher B

14-7049 was used in Flow cytometry/Cell sorting to demonstrate the consequences for mice with mutations in CTLA4, an essential negative regulator of immune responses.

Mon Dec 01 00:00:00 EST 2014

Akt-dependent enhanced migratory capacity of Th17 cells from children with lupus nephritis.

Journal of immunology (Baltimore, Md. : 1950)

Kshirsagar S,Riedl M,Billing H,Tönshoff B,Thangavadivel S,Steuber C,Staude H,Wechselberger G,Edelbauer M

14-7049 was used in Flow cytometry/Cell sorting to determine that therapeutic modulation of Akt activity could suppress chronic inflammatory processes in children with lupus.

Sat Nov 15 00:00:00 EST 2014

B cells in T follicular helper cell development and function: separable roles in delivery of ICOS ligand and antigen.

Journal of immunology (Baltimore, Md. : 1950)

Weinstein JS,Bertino SA,Hernandez SG,Poholek AC,Teplitzky TB,Nowyhed HN,Craft J

14-7049 was used in Flow cytometry/Cell sorting to study the role of B cells in the development of Tfh cells and germinal centres.

Tue Apr 01 00:00:00 EDT 2014

Potential role of IL-17-producing iNKT cells in type 1 diabetes.

PloS one

Li S,Joseph C,Becourt C,Klibi J,Luce S,Dubois-Laforgue D,Larger E,Boitard C,Benlagha K

14-7049 was used in Flow cytometry/Cell sorting to investigate the status and potential role of iNKT17 in type 1 diabetes.

Thu Jan 22 00:00:00 EST 2015

Rapid, multiparameter profiling of cellular secretion using silicon photonic microring resonator arrays.

Journal of the American Chemical Society

Luchansky MS,Bailey RC

Published figure using IL-4 monoclonal antibody (Product # 14-7049-81) in Radioimmune assays

Wed Dec 21 00:00:00 EST 2011

Simian immunodeficiency virus-induced changes in T cell cytokine responses in cynomolgus macaques with latent Mycobacterium tuberculosis infection are associated with timing of reactivation.

Journal of immunology (Baltimore, Md. : 1950)

Mattila JT,Diedrich CR,Lin PL,Phuah J,Flynn JL

14-7049 was used in Flow cytometry/Cell sorting to investigate alterations in multifuctional T cell responses during HIV-M. tuberculosis coinfection in an animal model.

Tue Mar 15 00:00:00 EDT 2011

Simian immunodeficiency virus-induced changes in T cell cytokine responses in cynomolgus macaques with latent Mycobacterium tuberculosis infection are associated with timing of reactivation.

Journal of immunology (Baltimore, Md. : 1950)

Mattila JT,Diedrich CR,Lin PL,Phuah J,Flynn JL

14-7049 was used in Flow cytometry/Cell sorting to investigate alterations in multifuctional T cell responses during HIV-M. tuberculosis coinfection in an animal model.

Tue Mar 15 00:00:00 EDT 2011

Anticardiac myosin immunity and chronic allograft vasculopathy in heart transplant recipients.

Journal of immunology (Baltimore, Md. : 1950)

Kalache S,Dinavahi R,Pinney S,Mehrotra A,Cunningham MW,Heeger PS

14-7049 was used in an ELISA assay to investigate the pathogenesis of chronic allograft vasculopathy in heart transplant failure.

Fri Jul 15 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: B cell growth factor 1; B-cell stimulatory factor 1; B_cell stimulatory factor 1; Binetrakin; BSF-1; H-IL-4; IL-4; ILN; Interleukin; interleukin 4 variant 2; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402; Pitrakinra

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Gene Aliases: BCGF-1; BCGF1; BSF-1; BSF1; IL-4; IL4

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UniProt ID: (Human) P05112

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Entrez Gene ID: (Human) 3565

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Function(s)

cytokine activity interleukin-4 receptor binding protein binding growth factor activity interleukin superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-4 Monoclonal Antibody (8D4-8), eBioscience™

IL-4 Antibody (14-7049-81) in WB, Flow

Product Details

14-7049-81

Western Blot (WB)

Flow Cytometry (Flow)

ELISA (ELISA)

Radioimmune Assays (RIA)

T-Cell Activation (TCA)

Affinity Purification (AP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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