Invitrogen

IL-10 Monoclonal Antibody (JES3-9D7), eBioscience™

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This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

14 Published Figures

33 References

View all (98) IL-10 antibodies

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IL-10 Antibody (14-7108-81) in WB

Western blot was performed using Anti-IL-10 Monoclonal Antibody (JES3-9D7), eBioscience™ (Product # 14-7108-81, 14-7108-85) and a 18kDa band corresponding to Interleukin-10 was observed in Human rec. IL10 as compared to Human rec. IL6 which was used as a negative control. Mouse rec. IL10 was used to test species reactivity. 250 ng Human rec. IL10 (Lane 1), Human rec. IL6 (Lane 2), Mouse rec. IL10 (Lane 3) were electrophoresed using NuPAGE™ 12%, Bis-Tris gel (Product # NP0341BOX). Resolved proteins were then transferred onto a nitrocellulos... View More

IL-10 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

IL-10 Antibody — Figure 6 (A) Ovarian ET cytokine expression upon treatment with an AhR antagonist. IL-4, IL-10, IL-12, IL-17, GM-CSF, IFN- , MCP-1/CCL2, MIP-1 /CCL4 and TNF-alpha protein expression in supernatants of untreated (control) or ANF-treated ovarian ET cultures measured by ELISA 48 hr after exposure to the AhR-antagonist ANF (15 uM). Control protein concentration was assigned a value of 1 and is illustrated as a horizontal line for presentation purposes. Sample size was 4 patients/group. The values are the mean and 95% confidence intervals. * p<0.05 (t-test). ( B ) Reduced PDT of endometrium stromal cells exposed to FICZ-activated MC-conditioned medium. Endometrium-derived stromal cells were cultured in conditioned medium (CM) of the HMC-1 MC line treated without (CM-Unstim) or with FICZ (CM-FICZ) or PMA and ionomycin (CM-PMA+Iono) (for details, see Material and Methods section). Results are expressed as the median and IQR (n = 3), * p<0.05 (Dunn's Test) vs CM-Unstim. {TM}

Product Details

14-7108-81

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Flow Cytometry (Flow)

View 18 publications 18 publications

ELISA (ELISA)

1-4 µg/mL

View 8 publications 8 publications

Neutralization (Neu)

View 2 publications 2 publications

Functional Assay (Functional)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Cynomolgus monkey, Human, Mouse, Non-human primate

Host/Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

JES3-9D7

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468438

Product Specific Information

Description: The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).

Applications Reported: The JES3-9D7 antibody has been reported for use in capture of human IL-10 by ELISA, intracellular flow cytometric analysis, and for neutralizing IL-10 bioactivity. (Use Functional Grade purified for functional studies Product # 16-7108).

Applications Tested: The JES3-9D7 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interleukin-10 (IL-10) in combination with the biotin JES3-12G8 (Product # 13-7109-81) antibody for detection and recombinant human IL-10 (14-8109) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Histological changes of cervical tumours following Zanthoxylum acanthopodium DC treatment, and its impact on cytokine expression.

Saudi journal of biological sciences

Simanullang RH,Situmorang PC,Herlina M,Silalahi B,Manurung SS

Published figure using IL-10 monoclonal antibody (Product # 14-7108-81) in Immunohistochemistry

Fri Apr 01 00:00:00 EDT 2022

Circulating Regulatory B-Lymphocytes in Patients with Acute Myocardial Infarction: A Pilot Study.

Journal of cardiovascular development and disease

Volodarsky I,Shimoni S,Haberman D,Mirkin V,Fabrikant Y,Yoskovich Mashriki T,Zalik A,George J

Published figure using IL-10 monoclonal antibody (Product # 14-7108-81) in Flow Cytometry

Thu Dec 22 00:00:00 EST 2022

Induction of cytotoxic effector cells towards cholangiocellular, pancreatic, and colorectal tumor cells by activation of the immune checkpoint CD40/CD40L on dendritic cells.

Cancer immunology, immunotherapy : CII

Sadeghlar F,Vogt A,Mohr RU,Mahn R,van Beekum K,Kornek M,Weismüller TJ,Branchi V,Matthaei H,Toma M,Schmidt-Wolf IGH,Kalff JC,Strassburg CP,González-Carmona MA

14-7108-81 was used in ELISA to point to the promising approach for DC-based immunotherapy of gastrointestinal malignances by activating the CD40/CD40L immune checkpoint.

Sat May 01 00:00:00 EDT 2021

Induction of cytotoxic effector cells towards cholangiocellular, pancreatic, and colorectal tumor cells by activation of the immune checkpoint CD40/CD40L on dendritic cells.

Cancer immunology, immunotherapy : CII

Sadeghlar F,Vogt A,Mohr RU,Mahn R,van Beekum K,Kornek M,Weismüller TJ,Branchi V,Matthaei H,Toma M,Schmidt-Wolf IGH,Kalff JC,Strassburg CP,González-Carmona MA

14-7108-81 was used in ELISA to point to the promising approach for DC-based immunotherapy of gastrointestinal malignances by activating the CD40/CD40L immune checkpoint.

Sat May 01 00:00:00 EDT 2021

Early loss of T lymphocyte 4-1BB receptor expression is associated with higher short-term mortality in alcoholic hepatitis.

PloS one

Eriksen LL,Nielsen MA,Laursen TL,Deleuran B,Vilstrup H,Støy S

Published figure using IL-10 monoclonal antibody (Product # 14-7108-81) in Flow Cytometry

Mon Nov 29 00:00:00 EST 2021

Cysticercus cellulosae Regulates T-Cell Responses and Interacts With the Host Immune System by Excreting and Secreting Antigens.

Frontiers in cellular and infection microbiology

Fan X,Zhang Y,Ouyang R,Luo B,Li L,He W,Liu M,Jiang N,Yang F,Wang L,Zhou B

Published figure using IL-10 monoclonal antibody (Product # 14-7108-81) in Flow Cytometry

Wed Oct 20 00:00:00 EDT 2021

Altered T-Cell Subsets are Associated with Dysregulated Cytokine Secretion of CD4⁺ T Cells During HIV Infection.

Journal of inflammation research

Wang D,Jiang Y,Song Y,Zeng Y,Li C,Wang X,Liu Y,Xiao J,Kong Y,Zhao H

Published figure using IL-10 monoclonal antibody (Product # 14-7108-81) in Flow Cytometry

Wed Apr 27 00:00:00 EDT 2022

Complement Signals Determine Opposite Effects of B Cells in Chemotherapy-Induced Immunity.

Cell

Lu Y,Zhao Q,Liao JY,Song E,Xia Q,Pan J,Li Y,Li J,Zhou B,Ye Y,Di C,Yu S,Zeng Y,Su S

14-7108 was used in Immunohistochemistry to demonstrate a critical role of the B cell subset switch in chemotherapy response, which has implications in designing novel anti-cancer therapies.

Thu Mar 19 00:00:00 EDT 2020

Transdifferentiation of tumor infiltrating innate lymphoid cells during progression of colorectal cancer.

Cell research

Wang S,Qu Y,Xia P,Chen Y,Zhu X,Zhang J,Wang G,Tian Y,Ying J,Fan Z

14-7108-81 was used in Flow Cytometry to profile tumor infiltrating innate lymphoid cells in colorectal cancer at early and late stages.

Wed Jul 01 00:00:00 EDT 2020

CAIX Regulates GBM Motility and TAM Adhesion and Polarization through EGFR/STAT3 under Hypoxic Conditions.

International journal of molecular sciences

Huang BR,Liu YS,Lai SW,Lin HJ,Shen CK,Yang LY,Lu DY

Published figure using IL-10 monoclonal antibody (Product # 14-7108-81) in Flow Cytometry

Fri Aug 14 00:00:00 EDT 2020

Immune rebound associates with a favorable clinical response to autologous HSCT in systemic sclerosis patients.

Blood advances

Arruda LCM,Malmegrim KCR,Lima-Júnior JR,Clave E,Dias JBE,Moraes DA,Douay C,Fournier I,Moins-Teisserenc H,Alberdi AJ,Covas DT,Simões BP,Lansiaux P,Toubert A,Oliveira MC

14-7108 was used in Flow cytometry/Cell sorting to evaluate the immunological mechanisms associated with clinical outcomes after autologous hematopoietic stem cell transplantation (AHSCT), focusing on regulatory T- (Treg) and B- (Breg) cell immune reconstitution, 31 systemic sclerosis (SSc) patients underwent simultaneous clinical and immunological evaluations over 36-month posttransplantation follow-up.

Tue Jan 23 00:00:00 EST 2018

Multiple Sclerosis-Associated Changes in the Composition and Immune Functions of Spore-Forming Bacteria.

mSystems

Cekanaviciute E,Pröbstel AK,Thomann A,Runia TF,Casaccia P,Katz Sand I,Crabtree E,Singh S,Morrissey J,Barba P,Gomez R,Knight R,Mazmanian S,Graves J,Cree BAC,Zamvil SS,Baranzini SE

Published figure using IL-10 monoclonal antibody (Product # 14-7108-81) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues.

Laboratory investigation; a journal of technical methods and pathology

Mariuzzi L,Domenis R,Orsaria M,Marzinotto S,Londero AP,Bulfoni M,Candotti V,Zanello A,Ballico M,Mimmi MC,Calcagno A,Marchesoni D,Di Loreto C,Beltrami AP,Cesselli D,Gri G

14-7108 was used in Immunofluorescence to suggest a new mechanistic role of mast cells in the pathogenesis of endometriosis.

Thu Sep 01 00:00:00 EDT 2016

Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues.

Laboratory investigation; a journal of technical methods and pathology

Mariuzzi L,Domenis R,Orsaria M,Marzinotto S,Londero AP,Bulfoni M,Candotti V,Zanello A,Ballico M,Mimmi MC,Calcagno A,Marchesoni D,Di Loreto C,Beltrami AP,Cesselli D,Gri G

14-7108 was used in Immunofluorescence to suggest a new mechanistic role of mast cells in the pathogenesis of endometriosis.

Thu Sep 01 00:00:00 EDT 2016

Effects of B Cell Depletion on Early Mycobacterium tuberculosis Infection in Cynomolgus Macaques.

Infection and immunity

Phuah J,Wong EA,Gideon HP,Maiello P,Coleman MT,Hendricks MR,Ruden R,Cirrincione LR,Chan J,Lin PL,Flynn JL

14-7108 was used in Flow cytometry/Cell sorting to examine the contribution of B cells and humoural immunity to the control of tuberculosis in non-human primates during the acute phase of infection.

Sun May 01 00:00:00 EDT 2016

The regulation of cancer cell migration by lung cancer cell-derived exosomes through TGF-β and IL-10.

Oncology letters

Wang Y,Yi J,Chen X,Zhang Y,Xu M,Yang Z

Published figure using IL-10 monoclonal antibody (Product # 14-7108-81) in ELISA

Mon Feb 01 00:00:00 EST 2016

Heterologous Immunity between Adenoviruses and Hepatitis C Virus: A New Paradigm in HCV Immunity and Vaccines.

PloS one

Singh S,Vedi S,Samrat SK,Li W,Kumar R,Agrawal B

Published figure using IL-10 monoclonal antibody (Product # 14-7108-81) in Flow Cytometry

Tue Jul 19 00:00:00 EDT 2016

IL-10 Potentiates Differentiation of Human Induced Regulatory T Cells via STAT3 and Foxo1.

Journal of immunology (Baltimore, Md. : 1950)

Hsu P,Santner-Nanan B,Hu M,Skarratt K,Lee CH,Stormon M,Wong M,Fuller SJ,Nanan R

14-7108-81 was used in Flow cytometry/Cell sorting to investigate the action of IL-10 on regulatory T cells, and the potential therapeutic implications for the treatment of immune diseases that this may hold.

Thu Oct 15 00:00:00 EDT 2015

Role of dendritic cells in the pathogenesis of Whipple's disease.

Infection and immunity

Schinnerling K,Geelhaar-Karsch A,Allers K,Friebel J,Conrad K,Loddenkemper C,Kühl AA,Erben U,Ignatius R,Moos V,Schneider T

14-7108 was used in Flow cytometry/Cell sorting to indicate that dysfunctional IL-12 production by DC provides suboptimal conditions for priming of T. whipplei-specific T cells.

Sun Feb 01 00:00:00 EST 2015

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Nature communications

Miles B,Miller SM,Folkvord JM,Kimball A,Chamanian M,Meditz AL,Arends T,McCarter MD,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using IL-10 monoclonal antibody (Product # 14-7108-81) in Flow Cytometry

Tue Oct 20 00:00:00 EDT 2015

Fiber composite slices for multiplexed immunoassays.

Biomicrofluidics

Kim J,Bae S,Song S,Chung K,Kwon S

14-7108 was used in Immuno-assay to describe a new type of low-cost fabrication method for a scalable immunoassay platform based on cotton threads.

Wed Jul 01 00:00:00 EDT 2015

Highly heterogeneous, activated, and short-lived regulatory T cells during chronic filarial infection.

European journal of immunology

Metenou S,Coulibaly YI,Sturdevant D,Dolo H,Diallo AA,Soumaoro L,Coulibaly ME,Kanakabandi K,Porcella SF,Klion AD,Nutman TB

14-7108 was used in Flow cytometry/Cell sorting to study how in filarial infection, the expanded Treg-cell populations are heterogeneous, short-lived, activated, and express higher levels of molecules known to modulate immune responsiveness.

Tue Jul 01 00:00:00 EDT 2014

Human CD141+ dendritic cells induce CD4+ T cells to produce type 2 cytokines.

Journal of immunology (Baltimore, Md. : 1950)

Yu CI,Becker C,Metang P,Marches F,Wang Y,Toshiyuki H,Banchereau J,Merad M,Palucka AK

14-7108-81 was used in Flow Cytometry to study how naive T cell priming by different subpopulations of dendritic cell influences effector T cell outcome.

Sat Nov 01 00:00:00 EDT 2014

Protein microarray analysis reveals BAFF-binding autoantibodies in systemic lupus erythematosus.

The Journal of clinical investigation

Price JV,Haddon DJ,Kemmer D,Delepine G,Mandelbaum G,Jarrell JA,Gupta R,Balboni I,Chakravarty EF,Sokolove J,Shum AK,Anderson MS,Cheng MH,Robinson WH,Browne SK,Holland SM,Baechler EC,Utz PJ

14-7108 was used in an ELISA assay to study how serum factor protein microarrays facilitate the detection of auto-antibody reactivity to serum factors in human samples.

Sun Dec 01 00:00:00 EST 2013

Gliomas promote immunosuppression through induction of B7-H1 expression in tumor-associated macrophages.

Clinical cancer research : an official journal of the American Association for Cancer Research

Bloch O,Crane CA,Kaur R,Safaee M,Rutkowski MJ,Parsa AT

14-7108 was used in Neutralization experiments to demonstrate that gliomas can upregulate B7-H1 expression in circulating monocytes and tumor-infiltrative macrophages through modulation of autocrine/paracrine IL-10 signaling.

Sat Jun 15 00:00:00 EDT 2013

The Pseudomonas aeruginosa PhoP-PhoQ two-component regulatory system is induced upon interaction with epithelial cells and controls cytotoxicity and inflammation.

Infection and immunity

Gellatly SL,Needham B,Madera L,Trent MS,Hancock RE

14-7108 was used in an ELISA assay to assess how disruption of the regulatory system, PhoPQ, in gram-negative species of bacteria affects virulence.

Sat Sep 01 00:00:00 EDT 2012

CD4 T cell depletion exacerbates acute Mycobacterium tuberculosis while reactivation of latent infection is dependent on severity of tissue depletion in cynomolgus macaques.

AIDS research and human retroviruses

Lin PL,Rutledge T,Green AM,Bigbee M,Fuhrman C,Klein E,Flynn JL

14-7108 was used in Flow cytometry/Cell sorting to study initial CD4 T cell protection against acute M.tuberculosis infection and reactivation upon latent infection.

Sat Dec 01 00:00:00 EST 2012

CD4 T cell depletion exacerbates acute Mycobacterium tuberculosis while reactivation of latent infection is dependent on severity of tissue depletion in cynomolgus macaques.

AIDS research and human retroviruses

Lin PL,Rutledge T,Green AM,Bigbee M,Fuhrman C,Klein E,Flynn JL

14-7108 was used in Flow cytometry/Cell sorting to study initial CD4 T cell protection against acute M.tuberculosis infection and reactivation upon latent infection.

Sat Dec 01 00:00:00 EST 2012

Neonatal plasma polarizes TLR4-mediated cytokine responses towards low IL-12p70 and high IL-10 production via distinct factors.

PloS one

Belderbos ME,Levy O,Stalpers F,Kimpen JL,Meyaard L,Bont L

14-7108 was used in an ELISA assay to conclude that human neonatal plasma contains at least two distinct factors that suppress TLR4-mediated IL-12p70 production or induce IL-10 or production.

Mon Jul 23 00:00:00 EDT 2012

Induction of cytokines in a macrophage cell line by proteins of Clostridium difficile.

FEMS immunology and medical microbiology

Vohra P,Poxton IR

14-7108 was used in an ELISA assay to assess the induction of cytokines in a macrophage cell line in response to different antigens prepared from five Clostridium difficile strains.

Fri Jun 01 00:00:00 EDT 2012

Regulatory B cell production of IL-10 inhibits lymphoma depletion during CD20 immunotherapy in mice.

The Journal of clinical investigation

Horikawa M,Minard-Colin V,Matsushita T,Tedder TF

14-7108 was used in Flow cytometry/Cell sorting to investigate whether heterogeneity in endogenous tissue CD20+ B cell depletion influences in vivo lymphoma therapy, showing that production of IL-10 inhibits lymphoma depletion.

Tue Nov 01 00:00:00 EDT 2011

Simian immunodeficiency virus-induced changes in T cell cytokine responses in cynomolgus macaques with latent Mycobacterium tuberculosis infection are associated with timing of reactivation.

Journal of immunology (Baltimore, Md. : 1950)

Mattila JT,Diedrich CR,Lin PL,Phuah J,Flynn JL

14-7108 was used in Flow cytometry/Cell sorting to investigate alterations in multifuctional T cell responses during HIV-M. tuberculosis coinfection in an animal model.

Tue Mar 15 00:00:00 EDT 2011

Simian immunodeficiency virus-induced changes in T cell cytokine responses in cynomolgus macaques with latent Mycobacterium tuberculosis infection are associated with timing of reactivation.

Journal of immunology (Baltimore, Md. : 1950)

Mattila JT,Diedrich CR,Lin PL,Phuah J,Flynn JL

14-7108 was used in Flow cytometry/Cell sorting to investigate alterations in multifuctional T cell responses during HIV-M. tuberculosis coinfection in an animal model.

Tue Mar 15 00:00:00 EDT 2011

Abnormally high levels of virus-infected IFN-gamma+ CCR4+ CD4+ CD25+ T cells in a retrovirus-associated neuroinflammatory disorder.

PloS one

Yamano Y,Araya N,Sato T,Utsunomiya A,Azakami K,Hasegawa D,Izumi T,Fujita H,Aratani S,Yagishita N,Fujii R,Nishioka K,Jacobson S,Nakajima T

14-7108 was used in Flow cytometry/Cell sorting to distinguish the HTLV-1 infected T cell subset contributing to neuroinflammation in HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP).

Wed Aug 05 00:00:00 EDT 2009

Granzyme B production distinguishes recently activated CD8(+) memory cells from resting memory cells.

Cellular immunology

Nowacki TM,Kuerten S,Zhang W,Shive CL,Kreher CR,Boehm BO,Lehmann PV,Tary-Lehmann M

Tue May 01 00:00:00 EDT 2007

Mycobacterium bovis BCG attenuates surface expression of mature class II molecules through IL-10-dependent inhibition of cathepsin S.

Journal of immunology (Baltimore, Md. : 1950)

Sendide K,Deghmane AE,Pechkovsky D,Av-Gay Y,Talal A,Hmama Z

Sat Oct 15 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor

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Gene Aliases: CSIF; GVHDS; IL-10; IL10; IL10A; TGIF

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UniProt ID: (Human) P22301

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Entrez Gene ID: (Human) 3586

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Function(s)

cytokine activity interleukin-10 receptor binding protein binding growth factor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-10 Monoclonal Antibody (JES3-9D7), eBioscience™

IL-10 Antibody (14-7108-81) in WB

Product Details

14-7108-81

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Functional Assay (Functional)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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