Invitrogen

IFN gamma Monoclonal Antibody (MD-1), eBioscience™

2 Published Figures

13 References

View all (214) IFN gamma antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (2)

IFN gamma Antibody in Immunostaining (IS)

FIGURE: 1 / 3

IFN gamma Antibody (14-7317-85) in IS

Human PBMCs were activated with PMA/Ionomycin in Human IFN gamma ELISPOT assay. Control well is medium alone.

IFN gamma Antibody in Flow Cytometry (Flow)

IFN gamma Antibody in Neutralization (Neu)

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Product Details

14-7317-85

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Flow Cytometry (Flow)

Assay-Dependent

View 1 publication 1 publication

ELISA (ELISA)

View 2 publications 2 publications

Neutralization (Neu)

View 4 publications 4 publications

Functional Assay (Functional)

View 4 publications 4 publications

T-Cell Activation (TCA)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Immunostaining (IS)

1-4 µg/mL

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Cynomolgus monkey, Human, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

MD-1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468474

Product Specific Information

Description: The MD-1 antibody reacts with human, rhesus, cynomolgus and baboon interferon-gamma (IFN gamma), the signature Th1 cytokine, which is produced mainly by activated T cells, NK cells and B cells. IFN gamma is a dimeric protein. IFN gamma has antiviral and antiparasitic activities and also inhibits the proliferation of normal and transformed cells.

Applications Reported: The MD-1 antibody has been reported for use in intracellular flow cytometric analysis, cytokine neutralization, immunohistochemical staining, ELISA, and ELISPOT. (Fluorochrome conjugated MD-1 is recommended for use in intracellular flow cytometry.) (Please use Functional Grade purified MD-1, Product # 16-7317, in functional assays).

Applications Tested: The MD-1 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human interferon gamma (IFN gamma) in combination with the biotinylated 4S.B3 (Product # 13-7319-81) antibody for detection and recombinant human IFN gamma (39-8319) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.

The FG Purified format of the MD-1 antibody has been tested as the capture antibody in an ELISPOT assay (in conjunction with the biotinylated 4S.B3 antibody) and for neutralization of IFN gamma biological activity. A suitable range of concentrations of this antibody for ELISPOT capture is 1-4 µg/mL.

For in vitro neutralization, the MD-1 antibody at 30 µg/mL has been found to neutralize by 50% the effect of 1 ng/mL human IFN gamma. A more potent neutralizing antibody is clone NIB42.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Serum Amyloid A Proteins Induce Pathogenic Th17 Cells and Promote Inflammatory Disease.

Cell

Lee JY,Hall JA,Kroehling L,Wu L,Najar T,Nguyen HH,Lin WY,Yeung ST,Silva HM,Li D,Hine A,Loke P,Hudesman D,Martin JC,Kenigsberg E,Merad M,Khanna KM,Littman DR

14-7317-85 was used in T-Cell Activation to suggest that serum amyloid A proteins direct a pathogenic pro-inflammatory Th17 cell differentiation program and promote Th17-mediated inflammatory diseases.

Thu Jan 09 00:00:00 EST 2020

Dynamic single-cell phenotyping of immune cells using the microfluidic platform DropMap.

Nature protocols

Bounab Y,Eyer K,Dixneuf S,Rybczynska M,Chauvel C,Mistretta M,Tran T,Aymerich N,Chenon G,Llitjos JF,Venet F,Monneret G,Gillespie IA,Cortez P,Moucadel V,Pachot A,Troesch A,Leissner P,Textoris J,Bibette J,Guyard C,Baudry J,Griffiths AD,Védrine C

14-7317-85 was used in Functional assay to develop a microfluidic platform, DropMap, for the phenotyping of different immune cell types.

Tue Sep 01 00:00:00 EDT 2020

Ionizing Radiation Induces Morphological Changes and Immunological Modulation of Jurkat Cells.

Frontiers in immunology

Voos P,Fuck S,Weipert F,Babel L,Tandl D,Meckel T,Hehlgans S,Fournier C,Moroni A,Rödel F,Thiel G

14-7317-85 was used in ELISA, Flow Cytometry, Immunohistochemistry (Frozen), Western Blot, Immunocytochemistry to reveal that ionizing radiation impacts on immune activation and functional properties of T-lymphocytes that may have implications in both toxic effects and treatment response in cancer patients.

Mon Jun 24 00:00:00 EDT 2019

IL-6/STAT3 pathway induced deficiency of RFX1 contributes to Th17-dependent autoimmune diseases via epigenetic regulation.

Nature communications

Zhao M,Tan Y,Peng Q,Huang C,Guo Y,Liang G,Zhu B,Huang Y,Liu A,Wang Z,Li M,Gao X,Wu R,Wu H,Long H,Lu Q

14-7317 was used in Functional assays to show reduced expression of the transcription factor RFX1 in CD4+ T cells from patients with systemic lupus erythematosus which leads to IL-17A overexpression through increased histone H3 acetylation and decreased DNA methylation.

Thu Feb 08 00:00:00 EST 2018

MAIT cells are activated during human viral infections.

Nature communications

van Wilgenburg B,Scherwitzl I,Hutchinson EC,Leng T,Kurioka A,Kulicke C,de Lara C,Cole S,Vasanawathana S,Limpitikul W,Malasit P,Young D,Denney L,Moore MD,Fabris P,Giordani MT,Oo YH,Laidlaw SM,Dustin LB,Ho LP,Thompson FM,Ramamurthy N,Mongkolsapaya J,Willberg CB,Screaton GR,Klenerman P

14-7317 was used in Blocking experiments to demonstrate how MAIT cells are activated following viral infections, suggesting a potential role in both host defence and immunopathology.

Thu Jun 23 00:00:00 EDT 2016

Targeted Vaccination against Human α-Lactalbumin for Immunotherapy and Primary Immunoprevention of Triple Negative Breast Cancer.

Cancers

Tuohy VK,Jaini R,Johnson JM,Loya MG,Wilk D,Downs-Kelly E,Mazumder S

14-7317 was used in in vitro experiments to investigate the use of retired self-proteins in the treatment of cancer, showing that vaccination against α-Lactalbumin can be used against triple negative breast cancers.

Thu Jun 16 00:00:00 EDT 2016

European journal of immunology

Shen H,Wang Y,Chen CY,Frencher J,Huang D,Yang E,Ryan-Payseur B,Chen ZW

14-7317 was used in Neutralization experiments to examine the involvement of cytokines in the adaptive immune response to bacterial infection.

Sun Feb 01 00:00:00 EST 2015

Protein microarray analysis reveals BAFF-binding autoantibodies in systemic lupus erythematosus.

The Journal of clinical investigation

Price JV,Haddon DJ,Kemmer D,Delepine G,Mandelbaum G,Jarrell JA,Gupta R,Balboni I,Chakravarty EF,Sokolove J,Shum AK,Anderson MS,Cheng MH,Robinson WH,Browne SK,Holland SM,Baechler EC,Utz PJ

14-7317 was used in an ELISA assay to study how serum factor protein microarrays facilitate the detection of auto-antibody reactivity to serum factors in human samples.

Sun Dec 01 00:00:00 EST 2013

Memory immune responses against pandemic (H1N1) 2009 influenza virus induced by a whole particle vaccine in cynomolgus monkeys carrying Mafa-A1*052:02.

PloS one

Arikata M,Itoh Y,Okamatsu M,Maeda T,Shiina T,Tanaka K,Suzuki S,Nakayama M,Sakoda Y,Ishigaki H,Takada A,Ishida H,Soda K,Pham VL,Tsuchiya H,Nakamura S,Torii R,Shimizu T,Inoko H,Ohkubo I,Kida H,Ogasawara K

14-7317 was used in an ELISA assay to examine the effectiveness of a vaccine derived from a virus library for pandemic virus infection in immunologically naïve macaques.

Wed Sep 19 00:00:00 EDT 2012

Differentiation, distribution and gammadelta T cell-driven regulation of IL-22-producing T cells in tuberculosis.

PLoS pathogens

Yao S,Huang D,Chen CY,Halliday L,Zeng G,Wang RC,Chen ZW

14-7317 was used in Neutralization experiments to investigate differentiation, distribution and immune regulation of human IL-22-producing T cells in infections.

Fri Feb 26 00:00:00 EST 2010

Differentiation, distribution and gammadelta T cell-driven regulation of IL-22-producing T cells in tuberculosis.

PLoS pathogens

Yao S,Huang D,Chen CY,Halliday L,Zeng G,Wang RC,Chen ZW

14-7317 was used in Neutralization experiments to investigate differentiation, distribution and immune regulation of human IL-22-producing T cells in infections.

Fri Feb 26 00:00:00 EST 2010

Phosphoantigen-activated V gamma 2V delta 2 T cells antagonize IL-2-induced CD4+CD25+Foxp3+ T regulatory cells in mycobacterial infection.

Blood

Gong G,Shao L,Wang Y,Chen CY,Huang D,Yao S,Zhan X,Sicard H,Wang R,Chen ZW

Thu Jan 22 00:00:00 EST 2009

Phosphoantigen-activated V gamma 2V delta 2 T cells antagonize IL-2-induced CD4+CD25+Foxp3+ T regulatory cells in mycobacterial infection.

Blood

Gong G,Shao L,Wang Y,Chen CY,Huang D,Yao S,Zhan X,Sicard H,Wang R,Chen ZW

Thu Jan 22 00:00:00 EST 2009

Programmed death (PD)-1:PD-ligand 1/PD-ligand 2 pathway inhibits T cell effector functions during human tuberculosis.

Journal of immunology (Baltimore, Md. : 1950)

Jurado JO,Alvarez IB,Pasquinelli V,Martínez GJ,Quiroga MF,Abbate E,Musella RM,Chuluyan HE,García VE

Tue Jul 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Immune interferon; Interferon gamma; Interferon y; Interferon γ; interferon, gamma; Interferonγ; M-IFN-g; R-IFN-g

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Gene Aliases: IFG; IFI; IFNG

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UniProt ID: (Human) P01579

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Entrez Gene ID: (Human) 3458

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Function(s)

cytokine activity interferon-gamma receptor binding cytokine interferon superfamily intercellular signal molecule

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IFN gamma Monoclonal Antibody (MD-1), eBioscience™

IFN gamma Antibody (14-7317-85) in IS

Product Details

14-7317-85

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Functional Assay (Functional)

T-Cell Activation (TCA)

Miscellaneous PubMed (Misc)

Immunostaining (IS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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