Invitrogen

IFN gamma Monoclonal Antibody (NIB42), eBioscience™

12 Published Figures

29 References

View all (214) IFN gamma antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (12)

IFN gamma Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

IFN gamma Antibody (14-7318-81) in ICC/IF

Autophagy 2015 - Published figure using IFN gamma monoclonal antibody (Product # 14-7318-81) in Immunocytochemistry

IFN gamma Antibody in Flow Cytometry (Flow)

IFN gamma Antibody in Flow Cytometry, Neutralization (Flow, Neu)

IFN gamma Antibody in Neutralization (Neu)

IFN gamma Antibody in Inhibition Assays (IA)

Product Image Gallery

Product Details

14-7318-81

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 9 publications 9 publications

ELISA (ELISA)

2-8 µg/mL

View 6 publications 6 publications

Neutralization (Neu)

View 8 publications 8 publications

Functional Assay (Functional)

View 2 publications 2 publications

Inhibition Assays (IA)

View 2 publications 2 publications

Affinity Purification (AP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

NIB42

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468475

Product Specific Information

Description: The NIB42 antibody reacts with human interferon-gamma (IFN-gamma). The NIB42 antibody is a neutralizing antibody. IFN-gamma is an ~20 kDa factor produced by activated T, B and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-gamma in synergy with other cytokines, such as TNF-alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN-gamma. Glycosylation of IFN-gamma does not affect its biological activity.

Applications Reported: The NIB42 antibody has been reported for use in capture of human IFN gamma by ELISA, and for neutralizing IFN gamma bioactivity. Use Functional Grade purified for functional assays Product # 16-7318).

Applications Tested: The NIB42 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interferon-gamma (IFN-g) in combination with the biotin 4S.B3 ( Product # 13-7319-81) antibody for detection and recombinant human IFNg (Product # 29-8319-65) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 2-8 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.For ELISPOT Capture, the alternative clone MD-1 is recommended.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of DYRK1B suppresses inflammation in allergic contact dermatitis model and Th1/Th17 immune response.

Scientific reports

Wongchang T,Pluangnooch P,Hongeng S,Wongkajornsilp A,Thumkeo D,Soontrapa K

Published figure using IFN gamma monoclonal antibody (Product # 14-7318-81) in Flow Cytometry

Sat Apr 29 00:00:00 EDT 2023

Boosting NAD preferentially blunts Th17 inflammation via arginine biosynthesis and redox control in healthy and psoriasis subjects.

Cell reports. Medicine

Han K,Singh K,Meadows AM,Sharma R,Hassanzadeh S,Wu J,Goss-Holmes H,Huffstutler RD,Teague HL,Mehta NN,Griffin JL,Tian R,Traba J,Sack MN

Published figure using IFN gamma monoclonal antibody (Product # 14-7318-81) in Inhibition Assays

Tue Sep 19 00:00:00 EDT 2023

Indole-3-pyruvic acid alleviates rheumatoid arthritis via the aryl hydrocarbon receptor pathway.

Annals of translational medicine

Huang T,Cheng L,Jiang Y,Zhang L,Qian L

14-7318-81 was used in Cell Culture to reveal the effect and mechanism of indole-3-pyruvic acid (IPA) in rheumatoid arthritis (RA).

Wed Mar 15 00:00:00 EDT 2023

Induction of cytotoxic effector cells towards cholangiocellular, pancreatic, and colorectal tumor cells by activation of the immune checkpoint CD40/CD40L on dendritic cells.

Cancer immunology, immunotherapy : CII

Sadeghlar F,Vogt A,Mohr RU,Mahn R,van Beekum K,Kornek M,Weismüller TJ,Branchi V,Matthaei H,Toma M,Schmidt-Wolf IGH,Kalff JC,Strassburg CP,González-Carmona MA

Published figure using IFN gamma monoclonal antibody (Product # 14-7318-81) in ELISA

Sat May 01 00:00:00 EDT 2021

Fasting-induced FOXO4 blunts human CD4⁺ T helper cell responsiveness.

Nature metabolism

Han K,Singh K,Rodman MJ,Hassanzadeh S,Wu K,Nguyen A,Huffstutler RD,Seifuddin F,Dagur PK,Saxena A,McCoy JP,Chen J,Biancotto A,Stagliano KER,Teague HL,Mehta NN,Pirooznia M,Sack MN

Published figure using IFN gamma monoclonal antibody (Product # 14-7318-81) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

Allogeneic ADSCs Induce the Production of Alloreactive Memory-CD8 T Cells through HLA-ABC Antigens.

Cells

Chang SH,Kim HJ,Park CG

14-7318-81 was used in Neutralization to study human leukocyte antigen expression in mesenchymal stem cells.

Mon May 18 00:00:00 EDT 2020

Allogeneic ADSCs induce CD8 T cell-mediated cytotoxicity and faster cell death after exposure to xenogeneic serum or proinflammatory cytokines.

Experimental & molecular medicine

Chang SH,Park CG

Published figure using IFN gamma monoclonal antibody (Product # 14-7318-81) in Flow Cytometry

Mon Mar 11 00:00:00 EDT 2019

Allogeneic ADSCs induce CD8 T cell-mediated cytotoxicity and faster cell death after exposure to xenogeneic serum or proinflammatory cytokines.

Experimental & molecular medicine

Chang SH,Park CG

Published figure using IFN gamma monoclonal antibody (Product # 14-7318-81) in Flow Cytometry

Mon Mar 11 00:00:00 EDT 2019

HTiP: High-Throughput Immunomodulator Phenotypic Screening Platform to Reveal IAP Antagonists as Anti-cancer Immune Enhancers.

Cell chemical biology

Mo X,Tang C,Niu Q,Ma T,Du Y,Fu H

14-7318 was used in an ELISA assay to develop a high-throughput immunomodulator phenotypic screening platform, HTiP, which integrates the immune and cancer cell co-culture system with imaging- and biochemical-based multiplexed readouts.

Thu Mar 21 00:00:00 EDT 2019

Th17 Responses to Collagen Type V, kα1-Tubulin, and Vimentin Are Present Early in Human Development and Persist Throughout Life.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Sullivan JA,Jankowska-Gan E,Hegde S,Pestrak MA,Agashe VV,Park AC,Brown ME,Kernien JF,Wilkes DS,Kaufman DB,Greenspan DS,Burlingham WJ

Published figure using IFN gamma monoclonal antibody (Product # 14-7318-81) in Neutralization

Sat Apr 01 00:00:00 EDT 2017

Activation-Induced Autophagy Is Preserved in CD4+ T-Cells in Familial Longevity.

The journals of gerontology. Series A, Biological sciences and medical sciences

Raz Y,Guerrero-Ros I,Maier A,Slagboom PE,Atzmon G,Barzilai N,Macian F

14-7318 was used in an ELISA assay to study the maintainance of autophagy in members of families with extended longevity improved T-cell function.

Fri Sep 01 00:00:00 EDT 2017

Interferon-γ Released by Activated CD8⁺ T Lymphocytes Impairs the Calcium Resorption Potential of Osteoclasts in Calcified Human Aortic Valves.

The American journal of pathology

Nagy E,Lei Y,Martínez-Martínez E,Body SC,Schlotter F,Creager M,Assmann A,Khabbaz K,Libby P,Hansson GK,Aikawa E

Published figure using IFN gamma monoclonal antibody (Product # 14-7318-81) in Neutralization

Thu Jun 01 00:00:00 EDT 2017

Trastuzumab upregulates expression of HLA-ABC and T cell costimulatory molecules through engagement of natural killer cells and stimulation of IFNγ secretion.

Oncoimmunology

Chaganty BK,Lu Y,Qiu S,Somanchi SS,Lee DA,Fan Z

14-7318-81 was used in Flow Cytometry to study the mechanism by which trastuzumab upregulates expression of HLA-ABC and T cell costimulatory molecules.

Fri Apr 01 00:00:00 EDT 2016

Induced PD-L1 expression mediates acquired resistance to agonistic anti-CD40 treatment.

Cancer immunology research

Zippelius A,Schreiner J,Herzig P,Müller P

14-7318 was used in Flow cytometry/Cell sorting to uncover a novel mechanism of acquired resistance to agonistic CD40 stimulation in tumours, and show how this mechanism can be successfully circumvented.

Sun Mar 01 00:00:00 EST 2015

IFNG-mediated immune responses enhance autophagy against Mycobacterium tuberculosis antigens in patients with active tuberculosis.

Autophagy

Rovetta AI,Peña D,Hernández Del Pino RE,Recalde GM,Pellegrini J,Bigi F,Musella RM,Palmero DJ,Gutierrez M,Colombo MI,García VE

Published figure using IFN gamma monoclonal antibody (Product # 14-7318-81) in Immunofluorescence

Tue Dec 08 00:00:00 EST 2015

IFNG-mediated immune responses enhance autophagy against Mycobacterium tuberculosis antigens in patients with active tuberculosis.

Autophagy

Rovetta AI,Peña D,Hernández Del Pino RE,Recalde GM,Pellegrini J,Bigi F,Musella RM,Palmero DJ,Gutierrez M,Colombo MI,García VE

Published figure using IFN gamma monoclonal antibody (Product # 14-7318-81) in Immunofluorescence

Tue Dec 08 00:00:00 EST 2015

Differential requirement for P2X7R function in IL-17 dependent vs. IL-17 independent cellular immune responses.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Sullivan JA,Jankowska-Gan E,Shi L,Roenneburg D,Hegde S,Greenspan DS,Wilkes DS,Denlinger LC,Burlingham WJ

Published figure using IFN gamma monoclonal antibody (Product # 14-7318-81) in Neutralization

Tue Jul 01 00:00:00 EDT 2014

Cytokine diversity in the Th1-dominated human anti-influenza response caused by variable cytokine expression by Th1 cells, and a minor population of uncommitted IL-2+IFNγ- Thpp cells.

PloS one

Deng N,Weaver JM,Mosmann TR

14-7318 was used in Flow cytometry/Cell sorting to study expression of IL-2 in T cell subsets during the anti-influenza response.

Thu Dec 18 00:00:00 EST 2014

C3a modulates IL-1β secretion in human monocytes by regulating ATP efflux and subsequent NLRP3 inflammasome activation.

Blood

Asgari E,Le Friec G,Yamamoto H,Perucha E,Sacks SS,Köhl J,Cook HT,Kemper C

14-7318 was used in Neutralization experiments to investigate how TLR signals and ATP availability are regulated during monocyte activation.

Thu Nov 14 00:00:00 EST 2013

Intercellular adhesion molecule 1 mediates migration of Th1 and Th17 cells across human retinal vascular endothelium.

Investigative ophthalmology & visual science

Bharadwaj AS,Schewitz-Bowers LP,Wei L,Lee RW,Smith JR

14-7318 was used in Flow cytometry/Cell sorting to investigate the ability of human Th1- and Th17-polarised cells to cross a simulated human retinal endothelium, and examined the role of IgG superfamily members in this process.

Wed Oct 23 00:00:00 EDT 2013

Interferon α-stimulated natural killer cells from patients with acute hepatitis C virus (HCV) infection recognize HCV-infected and uninfected hepatoma cells via DNAX accessory molecule-1.

The Journal of infectious diseases

Stegmann KA,Björkström NK,Ciesek S,Lunemann S,Jaroszewicz J,Wiegand J,Malinski P,Dustin LB,Rice CM,Manns MP,Pietschmann T,Cornberg M,Ljunggren HG,Wedemeyer H

14-7318 was used in Neutralization experiments to investigate interactions of HCV-infected cells and NK cells.

Tue May 01 00:00:00 EDT 2012

IL-17 and IFN-γ expression in lymphocytes from patients with active tuberculosis correlates with the severity of the disease.

Journal of leukocyte biology

Jurado JO,Pasquinelli V,Alvarez IB,Peña D,Rovetta AI,Tateosian NL,Romeo HE,Musella RM,Palmero D,Chuluyán HE,García VE

14-7318 was used in Flow cytometry/Cell sorting to investigate the induction and regulation of IFN-γ and IL-17 during active human Mycobacterium tuberculosis infection.

Fri Jun 01 00:00:00 EDT 2012

IL-17 and IFN-γ expression in lymphocytes from patients with active tuberculosis correlates with the severity of the disease.

Journal of leukocyte biology

Jurado JO,Pasquinelli V,Alvarez IB,Peña D,Rovetta AI,Tateosian NL,Romeo HE,Musella RM,Palmero D,Chuluyán HE,García VE

14-7318 was used in Flow cytometry/Cell sorting to investigate the induction and regulation of IFN-γ and IL-17 during active human Mycobacterium tuberculosis infection.

Fri Jun 01 00:00:00 EDT 2012

Human bone marrow stromal cells and skin fibroblasts inhibit natural killer cell proliferation and cytotoxic activity.

Cell transplantation

Pradier A,Passweg J,Villard J,Kindler V

14-7318 was used in Inhibition experiments to investigate the effect of allogenic mesenchymal stem cells and skin fibroblasts on natural killer cell function in vitro.

Thu Feb 09 00:00:00 EST 2012

Haemophilus ducreyi lipooligosaccharides induce expression of the immunosuppressive enzyme indoleamine 2,3-dioxygenase via type I interferons and tumor necrosis factor alpha in human dendritic cells.

Infection and immunity

Li W,Katz BP,Spinola SM

14-7318 was used in Blocking experiments to investigate the mechanisms by which H. ducreyi induces dendritic cells to express indoleamine 2,3-dioxygenase.

Mon Aug 01 00:00:00 EDT 2011

Role played by the programmed death-1-programmed death ligand pathway during innate immunity against Mycobacterium tuberculosis.

The Journal of infectious diseases

Alvarez IB,Pasquinelli V,Jurado JO,Abbate E,Musella RM,de la Barrera SS,García VE

14-7318 was used in Flow cytometry/Cell sorting to describe a novel inhibitory role played by PD-1:PD-L interactions in innate immunity in tuberculosis.

Sun Aug 15 00:00:00 EDT 2010

Human CD8+CXCR3+ T cells have the same function as murine CD8+CD122+ Treg.

European journal of immunology

Shi Z,Okuno Y,Rifa'i M,Endharti AT,Akane K,Isobe K,Suzuki H

14-7318-81 was used in Flow cytometry/Cell sorting to suggest that human CD8(+)CXCR3(+) T cells are the counterparts of murine CD8(+)CD122(+) Treg.

Sat Aug 01 00:00:00 EDT 2009

De novo generation and enhanced suppression of human CD4+CD25+ regulatory T cells by retinoic acid.

Journal of immunology (Baltimore, Md. : 1950)

Wang J,Huizinga TW,Toes RE

14-7318 was used in Neutralization experiments to investigate methods to convert T cells into Treg cells, showing that retinoic acid can be used for de novo generation of Tregs.

Tue Sep 15 00:00:00 EDT 2009

DNA-encoded antibody libraries: a unified platform for multiplexed cell sorting and detection of genes and proteins.

Journal of the American Chemical Society

Bailey RC,Kwong GA,Radu CG,Witte ON,Heath JR

14-7318 was used in Immuno-assay to develop a method for spatially multiplexed detection of ssDNAs and proteins as well as for cell sorting.

Wed Feb 21 00:00:00 EST 2007

Bioinformatics

Protein Aliases: H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Immune interferon; Interferon gamma; Interferon y; Interferon γ; interferon, gamma; Interferonγ; M-IFN-g; R-IFN-g

View more View less

Gene Aliases: IFG; IFI; IFNG

View more View less

UniProt ID: (Human) P01579

View more View less

Entrez Gene ID: (Human) 3458

View more View less

Function(s)

cytokine activity interferon-gamma receptor binding cytokine interferon superfamily intercellular signal molecule

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IFN gamma Monoclonal Antibody (NIB42), eBioscience™

IFN gamma Antibody (14-7318-81) in ICC/IF

Product Details

14-7318-81

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Functional Assay (Functional)

Inhibition Assays (IA)

Affinity Purification (AP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!