Invitrogen

CD45 Monoclonal Antibody (CD45-2B11), eBioscience™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

7 Published Figures

7 References

View all (407) CD45 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD45 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 13

CD45 Antibody (14-9457-82) in IHC (P)

Immunohistochemical analysis of CD45 was performed using formalin-fixed paraffin-embedded human tonsil tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a microwave at 100 degree Celsius for 10 minutes. Following ant... View More

CD45 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CD45 Antibody — Antibody clone (CD45-2B11) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Lossof signal was observed for target protein in CD45-2B11 KOcells (blue histogram) compared to the control Cas9cells (pink histogram) using CD45 antibody (CD45-2B11). Yellow histogram represents staining with the isotype control. {KO}

Product Details

14-9457-82

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

5-10 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-Dependent

Flow Cytometry (Flow)

0.25 µg/test

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

CD45-2B11

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11063696

Product Specific Information

Description: The CD45-2B11 monoclonal antibody reacts with human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in lymphocyte proliferation and differentiation. The 2B11 antibody is useful for recognition of normal and neoplastic lymphoid cells.

Applications Reported: This CD45-2B11 antibody has been reported for use in flow cytometric analysis, immunoblotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.

Applications Tested: This CD45-2B11 antibody has been tested by immunohistochemisty on formalin-fixed paraffin embedded tissue using high pH antigen retrieval. This can be used at less than or equal to 10 µg/mL. This CD45-2B11 antibody has also been tested by western blot at 5 µg/mL and by flow cytometric analysis on human peripheral blood cells at 0.25 µg/test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Macrophage-derived IL-6 trans-signalling as a novel target in the pathogenesis of bronchopulmonary dysplasia.

The European respiratory journal

Hirani D,Alvira CM,Danopoulos S,Milla C,Donato M,Tian L,Mohr J,Dinger K,Vohlen C,Selle J,V Koningsbruggen-Rietschel S,Barbarino V,Pallasch C,Rose-John S,Odenthal M,Pryhuber GS,Mansouri S,Savai R,Seeger W,Khatri P,Al Alam D,Dötsch J,Alejandre Alcazar MA

14-9457-82 was used in Immunohistochemistry to investigate inflammatory pathways and therapeutic targets in severe clinical and experimental bronchopulmonary dysplasia.

Tue Feb 01 00:00:00 EST 2022

Macrophage-derived IL-6 trans-signalling as a novel target in the pathogenesis of bronchopulmonary dysplasia.

The European respiratory journal

14-9457-82 was used in Immunohistochemistry to investigate inflammatory pathways and therapeutic targets in severe clinical and experimental bronchopulmonary dysplasia.

Tue Feb 01 00:00:00 EST 2022

Exosomes derived from stem cells of human deciduous exfoliated teeth inhibit angiogenesis in vivo and in vitro via the transfer of miR-100-5p and miR-1246.

Stem cell research & therapy

Liu P,Zhang Q,Mi J,Wang S,Xu Q,Zhuang D,Chen W,Liu C,Zhang L,Guo J,Wu X

Published figure using CD45 monoclonal antibody (Product # 14-9457-82) in Flow Cytometry

Thu Mar 03 00:00:00 EST 2022

Ex vivo culture of intact human patient derived pancreatic tumour tissue.

Scientific reports

Kokkinos J,Sharbeen G,Haghighi KS,Ignacio RMC,Kopecky C,Gonzales-Aloy E,Youkhana J,Timpson P,Pereira BA,Ritchie S,Pandzic E,Boyer C,Davis TP,Butler LM,Goldstein D,McCarroll JA,Phillips PA

Published figure using CD45 monoclonal antibody (Product # 14-9457-82) in Immunohistochemistry

Thu Jan 21 00:00:00 EST 2021

Ex vivo culture of intact human patient derived pancreatic tumour tissue.

Scientific reports

Kokkinos J,Sharbeen G,Haghighi KS,Ignacio RMC,Kopecky C,Gonzales-Aloy E,Youkhana J,Timpson P,Pereira BA,Ritchie S,Pandzic E,Boyer C,Davis TP,Butler LM,Goldstein D,McCarroll JA,Phillips PA

Published figure using CD45 monoclonal antibody (Product # 14-9457-82) in Immunohistochemistry

Thu Jan 21 00:00:00 EST 2021

Heterogeneous nuclear ribonucleoprotein A2/B1 is a negative regulator of human breast cancer metastasis by maintaining the balance of multiple genes and pathways.

EBioMedicine

Liu Y,Li H,Liu F,Gao LB,Han R,Chen C,Ding X,Li S,Lu K,Yang L,Tian HM,Chen BB,Li X,Xu DH,Deng XL,Shi SL

Published figure using CD45 monoclonal antibody (Product # 14-9457-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2020

Glucocorticoid receptor expression in resident and hematopoietic cells in IgG4-related disease.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Iguchi T,Takaori K,Mii A,Sato Y,Suzuki Y,Yoshifuji H,Seno H,Ogawa O,Omori K,Bessho K,Kondo S,Yoshizaki T,Nakashima H,Saito T,Mimori T,Haga H,Kawano M,Yanagita M

14-9457 was used in Immunohistochemistry-immunofluorescence to demonstrate that glucocorticoid receptor is abundantly expressed in the submandibular glands, kidneys, and retroperitoneum in IgG4-related disease patients, but not in patients with non-IgG4-related diseases, for which glucocorticoids are not effective.

Fri Jun 01 00:00:00 EDT 2018

Adoptive transfer of xenoantigen‑stimulated T cell receptor Vβ‑restricted human regulatory T cells prevents porcine islet xenograft rejection in humanized mice.

Molecular medicine reports

Jin X,Hu M,Gong L,Li H,Wang Y,Ji M,Li H

14-9457 was used in Immunohistochemistry-immunofluorescence to compare the ability of ex vivo expanded human xenoantigen stimulated Tregs and polyclonal Tregs to protect islet xenografts from rejection.

Thu Nov 01 00:00:00 EDT 2018

Heterogeneous fibroblasts underlie age-dependent tertiary lymphoid tissues in the kidney.

JCI insight

Sato Y,Mii A,Hamazaki Y,Fujita H,Nakata H,Masuda K,Nishiyama S,Shibuya S,Haga H,Ogawa O,Shimizu A,Narumiya S,Kaisho T,Arita M,Yanagisawa M,Miyasaka M,Sharma K,Minato N,Kawamoto H,Yanagita M

14-9457 was used in Immunohistochemistry to show how the inhibition of tertiary lymphoid tissue may constitute a therapeutic approach for treating acute kidney injury in the elderly.

Thu Jul 21 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD45 Monoclonal Antibody (CD45-2B11), eBioscience™

CD45 Antibody (14-9457-82) in IHC (P)

Product Details

14-9457-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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