Invitrogen

Snail1 Monoclonal Antibody (20C8), eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

13 Published Figures

15 References

View all (36) SNAIL antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Snail1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 17

Snail1 Antibody (14-9859-82) in IHC (P)

Immunohistochemistry on formalin-fixed paraffin embedded human infiltating ductal carcinoma using 5 µg/mL Mouse IgG2a Isotype Control (left) or 5 µg/mL Anti-Human Snail1 Purified (right) followed by anti-mouse IgG HRP and DAB visualization.Nuclei are counterstained with hematoxylin.

Snail1 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)

Snail1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Snail1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Snail1 Antibody in Immunocytochemistry (ICC/IF)

Snail1 Antibody in Western Blot, Immunohistochemistry, Immunoprecipitation (WB, IHC, IP)

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Product Details

14-9859-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

Immunocytochemistry (ICC/IF)

Assay-Dependent

View 4 publications 4 publications

ELISA (ELISA)

Assay-Dependent

Immunoprecipitation (IP)

Assay-Dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

20C8

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573045

Product Specific Information

Description: The monoclonal antibody 20C8 recognizes human Snail1, a 29 kDa zinc finger transcription factor. Snail1 and Slug both belong to a family of transcription factors that are responsible for inducing epithelial to mesenchymal transition (EMT) during normal organ development and tumor progression. Snail1 expression is localized to both the cytoplasm and nucleus and functions as a transcriptional repressor of E-cadherin, claudins, occludin, and desmoplakin. During embryonic development, Snail1 is localized to the mesoderm, while in the adult, expression is inducible and is usually limited to activated fibroblasts and some mesenchymal cells. Overexpression of Snail1 has been found in carcinomas and fibrosarcomas and may be used as an early marker for EMT.

Applications Reported: This 20C8 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded (IHC-P) tissue, ELISA, immunoprecipitation, western blotting, and immunocytochemistry.

Applications Tested: This 20C8 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded (FFPE) human breast cancer tissue using low pH antigen retrieval. The antibody can also be used following high pH antigen retrieval. This antibody can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Zinc finger protein SNAI1 (SNAIL)is a zinc finger transcriptional repressor which down regulates the expression of the ectodermal gene within the mesoderm and functions to form the mesoderm and neural crest. It is reported that SNAI1 acts as an important factor of tumor invasion as evidenced by its role in E-cadherin down-regulation and induction of epithelial-mesenchymal transition (EMT). In human breast cancer, the expression of SNAI1 and/or the homologous SNAI2 (Slug) has been associated with E-cadherin repression, local or distant metastasis, tumor recurrence, or poor prognosis in different tumor series. It is also reported that Snail 1 protein in the stromamay be anew putative prognosis marker for colon tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PRR15 deficiency facilitates malignant progression by mediating PI3K/Akt signaling and predicts clinical prognosis in triple-negative rather than non-triple-negative breast cancer.

Cell death & disease

Guo F,Ma J,Li C,Liu S,Wu W,Li C,Wang J,Wang J,Li Z,Zhai J,Sun F,Zhou Y,Guo C,Qian H,Xu B

Published figure using Snail1 monoclonal antibody (Product # 14-9859-82) in Western Blot

Tue Apr 18 00:00:00 EDT 2023

Aberrant Akt2 signaling in the RPE may contribute to retinal fibrosis process in diabetic retinopathy.

Cell death discovery

Daley R,Maddipatla V,Ghosh S,Chowdhury O,Hose S,Zigler JS,Sinha D,Liu H

14-9859-82 was used in Western Blot to find that in Akt2 cKO mice diabetes-induced increase of fibrosis markers, including collagen IV, Connective tissue growth factor (CTGF), fibronectin, and alpha-SMA was attenuated.

Thu Jul 13 00:00:00 EDT 2023

Lactate promotes endothelial-to-mesenchymal transition via Snail1 lactylation after myocardial infarction.

Science advances

Fan M,Yang K,Wang X,Chen L,Gill PS,Ha T,Liu L,Lewis NH,Williams DL,Li C

Published figure using Snail1 monoclonal antibody (Product # 14-9859-82) in Immunoprecipitation

Fri Feb 03 00:00:00 EST 2023

Lactate promotes endothelial-to-mesenchymal transition via Snail1 lactylation after myocardial infarction.

Science advances

Fan M,Yang K,Wang X,Chen L,Gill PS,Ha T,Liu L,Lewis NH,Williams DL,Li C

Published figure using Snail1 monoclonal antibody (Product # 14-9859-82) in Immunoprecipitation

Fri Feb 03 00:00:00 EST 2023

Lactate promotes endothelial-to-mesenchymal transition via Snail1 lactylation after myocardial infarction.

Science advances

Fan M,Yang K,Wang X,Chen L,Gill PS,Ha T,Liu L,Lewis NH,Williams DL,Li C

Published figure using Snail1 monoclonal antibody (Product # 14-9859-82) in Immunoprecipitation

Fri Feb 03 00:00:00 EST 2023

Lactate promotes endothelial-to-mesenchymal transition via Snail1 lactylation after myocardial infarction.

Science advances

Fan M,Yang K,Wang X,Chen L,Gill PS,Ha T,Liu L,Lewis NH,Williams DL,Li C

Published figure using Snail1 monoclonal antibody (Product # 14-9859-82) in Immunoprecipitation

Fri Feb 03 00:00:00 EST 2023

Low-Dose Albendazole Inhibits Epithelial-Mesenchymal Transition of Melanoma Cells by Enhancing Phosphorylated GSK-3β/Tyr216 Accumulation.

Journal of oncology

He Z,Lei S,Liang F,Tan L,Zhang W,Xie L,Zheng H,Lu Y

Published figure using Snail1 monoclonal antibody (Product # 14-9859-82) in Western Blot

Fri Dec 31 00:00:00 EST 2021

Circular RNA_0000629 Suppresses Bladder Cancer Progression Mediating MicroRNA-1290/CDC73.

Cancer management and research

Wang J,Luo J,Wu X,Gao Z

14-9859-82 was used in Western Blot to demonstrate that Circ_0000629 promotes the expression of CDC73 by competitively binding to miR-1290, thereby inhibiting the growth and metastasis of BC cells.

Thu Apr 21 00:00:00 EDT 2022

MiR-195 inhibits the ubiquitination and degradation of YY1 by Smurf2, and induces EMT and cell permeability of retinal pigment epithelial cells.

Cell death & disease

Fu SH,Lai MC,Zheng YY,Sun YW,Qiu JJ,Gui F,Zhang Q,Liu F

Published figure using Snail1 monoclonal antibody (Product # 14-9859-82) in Immunocytochemistry

Thu Jul 15 00:00:00 EDT 2021

MiR-195 inhibits the ubiquitination and degradation of YY1 by Smurf2, and induces EMT and cell permeability of retinal pigment epithelial cells.

Cell death & disease

Fu SH,Lai MC,Zheng YY,Sun YW,Qiu JJ,Gui F,Zhang Q,Liu F

Published figure using Snail1 monoclonal antibody (Product # 14-9859-82) in Immunocytochemistry

Thu Jul 15 00:00:00 EDT 2021

EIF3H promotes aggressiveness of esophageal squamous cell carcinoma by modulating Snail stability.

Journal of experimental & clinical cancer research : CR

Guo X,Zhu R,Luo A,Zhou H,Ding F,Yang H,Liu Z

14-9859 was used in Immunoprecipitation to reveal a critical EIF3H-Snail signaling axis in tumor aggressiveness in ESCC and provides EIF3H as a promising biomarker for ESCC treatment.

Mon Aug 31 00:00:00 EDT 2020

EIF3H promotes aggressiveness of esophageal squamous cell carcinoma by modulating Snail stability.

Journal of experimental & clinical cancer research : CR

Guo X,Zhu R,Luo A,Zhou H,Ding F,Yang H,Liu Z

14-9859 was used in Immunoprecipitation to reveal a critical EIF3H-Snail signaling axis in tumor aggressiveness in ESCC and provides EIF3H as a promising biomarker for ESCC treatment.

Mon Aug 31 00:00:00 EDT 2020

EIF3H promotes aggressiveness of esophageal squamous cell carcinoma by modulating Snail stability.

Journal of experimental & clinical cancer research : CR

Guo X,Zhu R,Luo A,Zhou H,Ding F,Yang H,Liu Z

14-9859 was used in Immunoprecipitation to reveal a critical EIF3H-Snail signaling axis in tumor aggressiveness in ESCC and provides EIF3H as a promising biomarker for ESCC treatment.

Mon Aug 31 00:00:00 EDT 2020

HOXC10 promotes migration and invasion via the WNT-EMT signaling pathway in oral squamous cell carcinoma.

Journal of Cancer

Dai BW,Yang ZM,Deng P,Chen YR,He ZJ,Yang X,Zhang S,Wu HJ,Ren ZH

Published figure using Snail1 monoclonal antibody (Product # 14-9859-82) in Immunofluorescence

Wed Sep 30 00:00:00 EDT 2020

PADI4‑mediated epithelial‑mesenchymal transition in lung cancer cells.

Molecular medicine reports

Liu M,Qu Y,Teng X,Xing Y,Li D,Li C,Cai L

14-9859-82 was used in Western Blot to evaluate the expression of protein‚arginine deiminase type‚4 and its associated molecular signalling in lung cancer metastasis.

Mon Apr 01 00:00:00 EDT 2019

A Role for βA3/A1-Crystallin in Type 2 EMT of RPE Cells Occurring in Dry Age-Related Macular Degeneration.

Investigative ophthalmology & visual science

Ghosh S,Shang P,Terasaki H,Stepicheva N,Hose S,Yazdankhah M,Weiss J,Sakamoto T,Bhutto IA,Xia S,Zigler JS,Kannan R,Qian J,Handa JT,Sinha D

14-9859-82 was used in Western Blot to study the role of βA3/A1-crystallin in age related macular degeneration.

Tue Mar 20 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: Protein sna; Protein snail homolog 1; Sna protein; snai; snail 1; snail 1 homolog; snail 1 zinc finger protein; snail 1, zinc finger protein; snail family zinc finger 1; snail homolog 1; Zinc finger protein SNAI1

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Gene Aliases: dJ710H13.1; SLUGH2; SNA; SNAH; SNAI1; SNAIL; SNAIL1

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UniProt ID: (Human) O95863

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Entrez Gene ID: (Human) 6615

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding protein binding kinase binding metal ion binding C2H2 zinc finger transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter osteoblast differentiation mesoderm formation epithelial to mesenchymal transition epithelial to mesenchymal transition involved in endocardial cushion formation positive regulation of epithelial to mesenchymal transition negative regulation of vitamin D biosynthetic process cell migration positive regulation of cell migration hair follicle morphogenesis negative regulation of DNA damage response, signal transduction by p53 class mediator positive regulation of transcription, DNA-templated palate development cartilage morphogenesis trophoblast giant cell differentiation negative regulation of cell differentiation involved in embryonic placenta development left/right pattern formation Notch signaling involved in heart development negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage regulation of bicellular tight junction assembly

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Snail1 Monoclonal Antibody (20C8), eBioscience™

Snail1 Antibody (14-9859-82) in IHC (P)

Product Details

14-9859-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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